Table 2.

Strains used in this study

StrainGenotypeFigure
DK2017 CLN3-6XHA::His3MX6, bar1 Fig. 1  
DK3822 NET1-6XHA::kanMX4, bar1 Figs. 2, 3, and 6  
DK4616 net1∆328-3XHA::URA, bar1 Figs. 2, 4, 7, and S1  
DK4661 net1∆328-3XHA::URA, pGAL-CDC20::kanMX5, bar1 Fig. 2  
DK4563 NET1-6XHA::kanMX4, net1∆328-3XHA::URA, bar1 Fig. 3  
DK4066 NET1-6XHA:: hphNT1 Figs. 5 and 6  
DK4180 NET1-6XHA:: hphNT1, sec6-4::kanMX6, swe1∆::His5 Figs. 5 and 6  
DK4005 NET1-6XHA::hphNT1, sec6-4::kanMX6, swe1∆::His5, cdc14TAB6-1 Fig. 6  
DK3838 NET1-6XHA::hphNT1, sec6-4::kanMX6, swe1∆::His5, bar1 Fig. 6  
DK4442 NET1-6XHA::hphNT1, sec6-4::kanMX6, swe1∆::His5, bfa1::caURA, bar1 Fig. 6  
DK4708 net1∆328-3XHA::URA, ypk2::His3, ypk1-as1, bar1 Fig. 7  
DK4624 net1∆328-3XHA::URA, cdc5-as1, bar1 Fig. S1  
DK4706 net1∆328-3XHA::URA, cdc15-as1, bar1 Fig. S1  
DK4654 net1∆328-3XHA::URA, sch9-as1 Fig. S1  
StrainGenotypeFigure
DK2017 CLN3-6XHA::His3MX6, bar1 Fig. 1  
DK3822 NET1-6XHA::kanMX4, bar1 Figs. 2, 3, and 6  
DK4616 net1∆328-3XHA::URA, bar1 Figs. 2, 4, 7, and S1  
DK4661 net1∆328-3XHA::URA, pGAL-CDC20::kanMX5, bar1 Fig. 2  
DK4563 NET1-6XHA::kanMX4, net1∆328-3XHA::URA, bar1 Fig. 3  
DK4066 NET1-6XHA:: hphNT1 Figs. 5 and 6  
DK4180 NET1-6XHA:: hphNT1, sec6-4::kanMX6, swe1∆::His5 Figs. 5 and 6  
DK4005 NET1-6XHA::hphNT1, sec6-4::kanMX6, swe1∆::His5, cdc14TAB6-1 Fig. 6  
DK3838 NET1-6XHA::hphNT1, sec6-4::kanMX6, swe1∆::His5, bar1 Fig. 6  
DK4442 NET1-6XHA::hphNT1, sec6-4::kanMX6, swe1∆::His5, bfa1::caURA, bar1 Fig. 6  
DK4708 net1∆328-3XHA::URA, ypk2::His3, ypk1-as1, bar1 Fig. 7  
DK4624 net1∆328-3XHA::URA, cdc5-as1, bar1 Fig. S1  
DK4706 net1∆328-3XHA::URA, cdc15-as1, bar1 Fig. S1  
DK4654 net1∆328-3XHA::URA, sch9-as1 Fig. S1  
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