Table 1.

Key resources table

Reagents or resource
Experimental models: Cell lines Source Identifier 
CFBE teton ΔF508-CFTR-HRP PMID: 29503157 N/A 
CFBE teton ΔF508-CFTR-3HA, YFP-H148Q/I152L/F46L PMID: 29503157 N/A 
CFBE ΔF508-CFTR-3HA PMID: 29503157 N/A 
CFBE teton HBH-ΔF508-CFTR PMID: 29503157 N/A 
CFBE teton ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
COS-7 JCRB cell bank Cat#JCRB9127 
BHK PMID: 29503157 N/A 
BHK HBH-ΔF508-CFTR-3HA PMID: 29503157 N/A 
293MSR Thermo Fisher Scientific Cat#R79507 
293MSR RNF5/185 DKO This paper N/A 
293MSR HBH-∆F508-CFTR-3HA This paper N/A 
293MSR RNF5/185 DKO HBH-∆F508-CFTR-3HA This paper N/A 
293MSR teton ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
BEAS-2B ECACC Cat#95102433 
BEAS-2B teton ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
Recombinant DNA Source Identifier 
pNUT ∆F508-CFTR-3HA PMID: 7691813 N/A 
pcDNA 6xmyc-HERC3 This paper N/A 
pcDNA 6xmyc-HERC3 ∆HECT This paper N/A 
pcDNA 6xmyc-HERC3 ∆RLD This paper N/A 
pcDNA 6xmyc-HERC3-C1018A This paper N/A 
pNUT ∆F508-CFTR-3HA-HiBiT(CT) This paper N/A 
pBiT1.1-N [TK/LgBiT] Promega Cat#N198A 
pBiT1.1-N [TK/CNXss-LgBiT-KDEL] This paper N/A 
pcDNA6 ∆F508-CFTR-HiBiT (Ex) This paper N/A 
pCS2+ FLAG-hPLIC-1(UBQLN1) Addgene Cat#8663 
pCS2+ FLAG-hPLIC-2(UBQLN2) Addgene Cat#8661 
pCS2+ FLAG-UBQLN2 ∆UBL This paper N/A 
pCS2+ FLAG-UBQLN2 ∆M This paper N/A 
pCS2+ FLAG-UBQLN2 ∆UBA This paper N/A 
pDONR221 Addgene Cat#3388 
pLX304 Addgene Cat#25890 
pLX303 HBH-∆F508 CFTR-3HA This paper N/A 
pLIX402 Addgene Cat#41394 
pLIX402 blast This paper N/A 
pLIX402 Nluc(CT) This paper N/A 
pLIX402 ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
pNUT-HBH-∆F508-CFTR-3HA PMID: 29503157 N/A 
pCMV-TCRα-HA PMID: 10601236 N/A 
pBiT2.1 TCRα-HiBiT This paper N/A 
pBiT2.1 Insig-1-HiBiT This paper N/A 
pBiT2.1 ∆Y490-ABCB1-HiBiT This paper N/A 
pNUT CFTR M1 (1–413 aa)-HiBiT This paper N/A 
pNUT ∆F508-CFTR M1N1 (1–635 aa)-HiBiT This paper N/A 
pNUT CFTR M2 (733–1218 aa)-3HA-HiBiT This paper N/A 
pBiT2.1 ∆F508-CFTR N1 (389–678 aa)-HiBiT This paper N/A 
pNUT N1303K-CFTR-3HA-HiBiT This paper N/A 
pEF6 D18G-TTR PMID: 17431395 N/A 
pEF6 D18G-TTR-FLAG PMID: 17431395 N/A 
pBiT2.1 ∆Y490-ABCB1 ∆M1 (1–379aa)-HiBiT This paper N/A 
pBiT2.1 ∆Y490-ABCB1 ∆M2 (681–1025aa)-HiBiT This paper N/A 
pBiT2.1 ∆Y490-ABCB1 (∆1-379)-CFTR-MSD1 (1–413 aa)-HiBiT (∆Y490-ABCB1-MSD1CFTR) This paper N/A 
pBiT2.1 ∆Y490-ABCB1 (∆681-1025)-CFTR-MSD2 (837–1156 aa)-HiBiT (∆Y490-ABCB1-MSD2CFTR) This paper N/A 
pSpCas9(BB)-2A-Puro (PX459) V2.0 Addgene Cat#62988 
pSpCas9(BB)-2A-Puro-RNF5 gRNA #1 This paper N/A 
pSpCas9(BB)-2A-Puro-RNF5 gRNA #2 This paper N/A 
pSpCas9(BB)-2A-Puro-RNF185 gRNA #1 This paper N/A 
pSpCas9(BB)-2A-Puro-RNF185 gRNA #2 This paper N/A 
pEU-E01-nbls-∆F508-CFTR (Biotin-∆F508-CFTR) This paper N/A 
pEU-E01-nbls-CFTR M1 (1-413) (Biotin-CFTR M1) This paper N/A 
pEU-E01-nbls-CFTR M2 (733-1218) (Biotin-CFTR M2) This paper N/A 
pEU-E01-nFLAG-DHFR (FLAG-DHFR) PMID: 27249653 N/A 
pEU-E01-nFLAG-HERC3 (FLAG-HERC3) This paper N/A 
pCold-His-SUMO-RNF185 ΔTM This paper N/A 
Antibodies Source Identifier 
HA (16B12) BioLegend Cat#901515 
Na+/K+-ATPase Santa Cruz Biotechnology Cat#sc-48345 
HERC3 (2C1) Abnova Cat#H00008916-M01A 
Ub (P4D1) Santa Cruz Biotechnology Cat#sc-8017 
Myc Wako Cat#017-21871 
RNF185 antiserum This paper N/A 
K48-Ub (Apu2) Merck Millipore Cat#05-1307 
K63-Ub (Apu3) Merck Millipore Cat#05-1308 
FLAG (1E6) Wako Cat#014-22383 
UBQLN2 (D7R2Z) Cell Signaling Technology Cat#85509 
CNX MBL Cat#PM060 
UBQLN1 (D3T7F) Cell Signaling Technology Cat#14526 
UBQLN4 Bethyl Laboratories Cat#A305-237A 
Prealbumine (TTR, E-1) Santa Cruz Biotechnology Cat#sc-377517 
HERC3 (2C1) Abnova Cat#H00008916-M01A 
HiBiT Promega Cat#N7200 
Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) Jackson Immuno Research Cat#115-035-166 
Peroxidase AffiniPure Donkey Anti- Rabbit IgG (H+L) Jackson Immuno Research Cat#711-035-152 
Chemicals, peptides, and recombinant proteins Source Identifier 
Lipofectamine RNAiMAX Transfection reagent Invitrogen Cat#13778150 
Doxycycline Hydrochloride n-Hydrate (Dox) FUJIFILM Wako Cat#049-31121 
8-cpt-cAMP Santa Cruz Biotechnology Cat#sc-201569A 
Forskolin FUJIFILM Wako Cat#067-02191 
3-isobutyl-1-methyl-xanthine (IBMX) FUJIFILM Wako Cat#095-03413 
Genistein FUJIFILM Wako Cat#073-05531 
Cycloheximide (CHX) FUJIFILM Wako Cat#037-20991 
MG-132 Cayman Cat#10012628 
N-Ethylmaleimide (NEM) FUJIFILM Wako Cat#054-02063 
Pepstatin A Peptide institute Cat#4397 
Leupeptin Hemisulfate monohydrate FUJIFILM Wako Cat#122-03751 
Benzylsulfonyl Fluoride (PMSF) FUJIFILM Wako Cat#164-12181 
Ponceau-S Sigma-Aldrich Cat#24-3875-5 
Ponceau-S Beacle, Inc Cat#BCL-PSS-01 
NeutrAvidin agarose Thermo Fisher Scientific Cat#29200 
VX-809 Cayman Cat#22196 
VX-661 Selleck Chemicals Cat#S7059 
VX-445 Selleck Chemicals Cat#S8851 
VX-770 Chemscene LLC Cat#CS-0497 
Nunc FluoroNunc/LumiNunc 96-Well Plates Thermo Fisher Scientific Cat#437796 
Avidin, NeutrAvidin Biotin-binding protein Invitrogen Cat#A2666 
Nunc MicroWell 96-Well, Nunclon Delta-Treated, Flat-Bottom Microplate Thermo Fisher Scientific Cat#136101 
Nano-Glo Endurazine Live Cell Substrate Promega Cat#N2571 
Albumin, from Bovine Serum, Fraction V pH7.0 (BSA) FUJIFILM Wako Cat#015-27053 
(+)-Biotin FUJIFILM Wako Cat#029-08713 
Urea FUJIFILM Wako Cat#217-00615 
DbeQ Selleck Cat#S7199 
CO2 independent medium Thermo Fisher Scientific Cat#18045088 
Polyethylenimine (PEI) Max Polyscience, Inc Cat#24765 
G418 FUJIFILM Wako Cat#074-05963 
Puromycin Sigma-Aldrich Cat#P9620-10ML 
Blasticidin S, Hydrochloride Funakoshi Cat#KK-400 
SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat#34580 
ImmunoStar Zeta FUJIFILM Wako Cat#295-72404 
ImmunoStar LD FUJIFILM Wako Cat#292-69903 
Oligonucleotides Source Identifier 
RNF5 gRNA#1: 5′-GCC​CAA​CGA​TCG​TGG​GCA​GG-3′ This paper N/A 
RNF5 gRNA#2: 5′-GAC​TCT​TAC​CTG​TAC​CCC​GG-3′ This paper N/A 
RNF185 gRNA#1: 5′-TGT​AGT​GGT​ACG​GTG​CAA​AC-3′ This paper N/A 
RNF185 gRNA#2: 5′-AAT​GGC​GCT​GGC​GAG​AGC​GG-3′ This paper N/A 
AllStars Neg. Control siRNA (siNT) Qiagen Cat#1027281 
HS_HERC3_4 siRNA: 5′-CAG​CCC​GAG​CTG​ATC​GCT​TTA-3′ Qiagen Cat#SI00107233 
HS_HERC3_5 siRNA: 5′-CAG​CGA​TCG​GAT​TCC​CAT​CTA-3′ Qiagen Cat#SI03066959 
HS_HERC3_8 siRNA: 5′-TTG​AGT​CTC​TAT​ATT​GTC​CAA-3′ Qiagen Cat#SI05092101 
Hs_RNF5_11 siRNA: 5′-CAG​CCA​GAG​AAG​AAT​CAG​TAT-3′ Qiagen Cat#SI05063114 
Hs_RNF5_6 siRNA: 5′-GGG​CGC​GAC​CTT​CGA​ATG​TAA-3′ Qiagen Cat#SI03221484 
Hs_RNF5_7 siRNA: 5′-AAC​GGC​AAG​AGT​GTC​CAG​TAT-3′ Qiagen Cat#SI04204116 
Hs_RNF185_2 siRNA: 5′-CAC​CTT​CTG​GAA​ATC​CGC​AAA-3′ Qiagen Cat#SI03059385 
Hs_RNF185_3 siRNA: 5′-CTC​CGA​GGC​AGT​AAT​AAC​TGA-3′ Qiagen Cat#SI03089982 
Hs_RNF185_8 siRNA: 5′-TCC​CAT​CAG​CTT​TCT​CCC​TAA-3′ Qiagen Cat#SI04959598 
Hs_RFFL_1 siRNA 5′-ATC​GGT​TTC​TTC​AGT​GCC​TTA-3′ Qiagen Cat#SI00148232 
Hs_RFFL_3 siRNA 5′-TCG​CAA​CTT​TGT​CAA​CTA​CAA-3′ Qiagen Cat#SI00148246 
Hs_RFFL_5 siRNA 5′-CTC​CAT​GAC​ATC​TCT​ACC​GAA-3′ Qiagen Cat#SI03089653 
RNF5 genome PCR primer pair: 5′-CCAGCCGAATGAGTTGGGTTAACAC-3′ (FW)
5′-GGCGAGAAACAGGAAGAGGGAATCC-3′ (Rev) 		This paper N/A 
RNF185 genome PCR primer pair: 5′-ACCTAGCAAGCTAGAGGCTG-3′ (FW)
5′-AGGGCTCCTGTAACCGTCAA-3′ (Rev) 		This paper N/A 
M13F sequence primer: 5′-GTA​AAA​CGA​CGG​CCA​GT-3′ This paper N/A 
M13R pUC sequence primer: 5′-CAG​GAA​ACA​GCT​ATG​AC-3′ This paper N/A 
HERC3 qPCR primer pair: 5′-GATCTGCCGAGAAAGCTATGG-3′ (FW)
5′-TTTGCCACCACACACCTTTAG-3′ (Rev) 		This paper N/A 
GAPDH qPCR primer pair: 5′-CATGAGAAGTATGACAACAGCCT-3′ (FW)
5′-AGTCCTTCCACGATACCAAAGT-3′ (Rev) 		This paper N/A 
Software and algorithms Source Identifier 
GraphPad Prism 8 GraphPad Software, Inc RRID:SCR_002798 
Evolution Capt Vilber-Lourmat N/A 
ImageQuant Cytiva RRID:SCR_014246 
Reagents or resource
Experimental models: Cell lines Source Identifier 
CFBE teton ΔF508-CFTR-HRP PMID: 29503157 N/A 
CFBE teton ΔF508-CFTR-3HA, YFP-H148Q/I152L/F46L PMID: 29503157 N/A 
CFBE ΔF508-CFTR-3HA PMID: 29503157 N/A 
CFBE teton HBH-ΔF508-CFTR PMID: 29503157 N/A 
CFBE teton ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
COS-7 JCRB cell bank Cat#JCRB9127 
BHK PMID: 29503157 N/A 
BHK HBH-ΔF508-CFTR-3HA PMID: 29503157 N/A 
293MSR Thermo Fisher Scientific Cat#R79507 
293MSR RNF5/185 DKO This paper N/A 
293MSR HBH-∆F508-CFTR-3HA This paper N/A 
293MSR RNF5/185 DKO HBH-∆F508-CFTR-3HA This paper N/A 
293MSR teton ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
BEAS-2B ECACC Cat#95102433 
BEAS-2B teton ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
Recombinant DNA Source Identifier 
pNUT ∆F508-CFTR-3HA PMID: 7691813 N/A 
pcDNA 6xmyc-HERC3 This paper N/A 
pcDNA 6xmyc-HERC3 ∆HECT This paper N/A 
pcDNA 6xmyc-HERC3 ∆RLD This paper N/A 
pcDNA 6xmyc-HERC3-C1018A This paper N/A 
pNUT ∆F508-CFTR-3HA-HiBiT(CT) This paper N/A 
pBiT1.1-N [TK/LgBiT] Promega Cat#N198A 
pBiT1.1-N [TK/CNXss-LgBiT-KDEL] This paper N/A 
pcDNA6 ∆F508-CFTR-HiBiT (Ex) This paper N/A 
pCS2+ FLAG-hPLIC-1(UBQLN1) Addgene Cat#8663 
pCS2+ FLAG-hPLIC-2(UBQLN2) Addgene Cat#8661 
pCS2+ FLAG-UBQLN2 ∆UBL This paper N/A 
pCS2+ FLAG-UBQLN2 ∆M This paper N/A 
pCS2+ FLAG-UBQLN2 ∆UBA This paper N/A 
pDONR221 Addgene Cat#3388 
pLX304 Addgene Cat#25890 
pLX303 HBH-∆F508 CFTR-3HA This paper N/A 
pLIX402 Addgene Cat#41394 
pLIX402 blast This paper N/A 
pLIX402 Nluc(CT) This paper N/A 
pLIX402 ∆F508-CFTR-3HA-Nluc(CT) This paper N/A 
pNUT-HBH-∆F508-CFTR-3HA PMID: 29503157 N/A 
pCMV-TCRα-HA PMID: 10601236 N/A 
pBiT2.1 TCRα-HiBiT This paper N/A 
pBiT2.1 Insig-1-HiBiT This paper N/A 
pBiT2.1 ∆Y490-ABCB1-HiBiT This paper N/A 
pNUT CFTR M1 (1–413 aa)-HiBiT This paper N/A 
pNUT ∆F508-CFTR M1N1 (1–635 aa)-HiBiT This paper N/A 
pNUT CFTR M2 (733–1218 aa)-3HA-HiBiT This paper N/A 
pBiT2.1 ∆F508-CFTR N1 (389–678 aa)-HiBiT This paper N/A 
pNUT N1303K-CFTR-3HA-HiBiT This paper N/A 
pEF6 D18G-TTR PMID: 17431395 N/A 
pEF6 D18G-TTR-FLAG PMID: 17431395 N/A 
pBiT2.1 ∆Y490-ABCB1 ∆M1 (1–379aa)-HiBiT This paper N/A 
pBiT2.1 ∆Y490-ABCB1 ∆M2 (681–1025aa)-HiBiT This paper N/A 
pBiT2.1 ∆Y490-ABCB1 (∆1-379)-CFTR-MSD1 (1–413 aa)-HiBiT (∆Y490-ABCB1-MSD1CFTR) This paper N/A 
pBiT2.1 ∆Y490-ABCB1 (∆681-1025)-CFTR-MSD2 (837–1156 aa)-HiBiT (∆Y490-ABCB1-MSD2CFTR) This paper N/A 
pSpCas9(BB)-2A-Puro (PX459) V2.0 Addgene Cat#62988 
pSpCas9(BB)-2A-Puro-RNF5 gRNA #1 This paper N/A 
pSpCas9(BB)-2A-Puro-RNF5 gRNA #2 This paper N/A 
pSpCas9(BB)-2A-Puro-RNF185 gRNA #1 This paper N/A 
pSpCas9(BB)-2A-Puro-RNF185 gRNA #2 This paper N/A 
pEU-E01-nbls-∆F508-CFTR (Biotin-∆F508-CFTR) This paper N/A 
pEU-E01-nbls-CFTR M1 (1-413) (Biotin-CFTR M1) This paper N/A 
pEU-E01-nbls-CFTR M2 (733-1218) (Biotin-CFTR M2) This paper N/A 
pEU-E01-nFLAG-DHFR (FLAG-DHFR) PMID: 27249653 N/A 
pEU-E01-nFLAG-HERC3 (FLAG-HERC3) This paper N/A 
pCold-His-SUMO-RNF185 ΔTM This paper N/A 
Antibodies Source Identifier 
HA (16B12) BioLegend Cat#901515 
Na+/K+-ATPase Santa Cruz Biotechnology Cat#sc-48345 
HERC3 (2C1) Abnova Cat#H00008916-M01A 
Ub (P4D1) Santa Cruz Biotechnology Cat#sc-8017 
Myc Wako Cat#017-21871 
RNF185 antiserum This paper N/A 
K48-Ub (Apu2) Merck Millipore Cat#05-1307 
K63-Ub (Apu3) Merck Millipore Cat#05-1308 
FLAG (1E6) Wako Cat#014-22383 
UBQLN2 (D7R2Z) Cell Signaling Technology Cat#85509 
CNX MBL Cat#PM060 
UBQLN1 (D3T7F) Cell Signaling Technology Cat#14526 
UBQLN4 Bethyl Laboratories Cat#A305-237A 
Prealbumine (TTR, E-1) Santa Cruz Biotechnology Cat#sc-377517 
HERC3 (2C1) Abnova Cat#H00008916-M01A 
HiBiT Promega Cat#N7200 
Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) Jackson Immuno Research Cat#115-035-166 
Peroxidase AffiniPure Donkey Anti- Rabbit IgG (H+L) Jackson Immuno Research Cat#711-035-152 
Chemicals, peptides, and recombinant proteins Source Identifier 
Lipofectamine RNAiMAX Transfection reagent Invitrogen Cat#13778150 
Doxycycline Hydrochloride n-Hydrate (Dox) FUJIFILM Wako Cat#049-31121 
8-cpt-cAMP Santa Cruz Biotechnology Cat#sc-201569A 
Forskolin FUJIFILM Wako Cat#067-02191 
3-isobutyl-1-methyl-xanthine (IBMX) FUJIFILM Wako Cat#095-03413 
Genistein FUJIFILM Wako Cat#073-05531 
Cycloheximide (CHX) FUJIFILM Wako Cat#037-20991 
MG-132 Cayman Cat#10012628 
N-Ethylmaleimide (NEM) FUJIFILM Wako Cat#054-02063 
Pepstatin A Peptide institute Cat#4397 
Leupeptin Hemisulfate monohydrate FUJIFILM Wako Cat#122-03751 
Benzylsulfonyl Fluoride (PMSF) FUJIFILM Wako Cat#164-12181 
Ponceau-S Sigma-Aldrich Cat#24-3875-5 
Ponceau-S Beacle, Inc Cat#BCL-PSS-01 
NeutrAvidin agarose Thermo Fisher Scientific Cat#29200 
VX-809 Cayman Cat#22196 
VX-661 Selleck Chemicals Cat#S7059 
VX-445 Selleck Chemicals Cat#S8851 
VX-770 Chemscene LLC Cat#CS-0497 
Nunc FluoroNunc/LumiNunc 96-Well Plates Thermo Fisher Scientific Cat#437796 
Avidin, NeutrAvidin Biotin-binding protein Invitrogen Cat#A2666 
Nunc MicroWell 96-Well, Nunclon Delta-Treated, Flat-Bottom Microplate Thermo Fisher Scientific Cat#136101 
Nano-Glo Endurazine Live Cell Substrate Promega Cat#N2571 
Albumin, from Bovine Serum, Fraction V pH7.0 (BSA) FUJIFILM Wako Cat#015-27053 
(+)-Biotin FUJIFILM Wako Cat#029-08713 
Urea FUJIFILM Wako Cat#217-00615 
DbeQ Selleck Cat#S7199 
CO2 independent medium Thermo Fisher Scientific Cat#18045088 
Polyethylenimine (PEI) Max Polyscience, Inc Cat#24765 
G418 FUJIFILM Wako Cat#074-05963 
Puromycin Sigma-Aldrich Cat#P9620-10ML 
Blasticidin S, Hydrochloride Funakoshi Cat#KK-400 
SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat#34580 
ImmunoStar Zeta FUJIFILM Wako Cat#295-72404 
ImmunoStar LD FUJIFILM Wako Cat#292-69903 
Oligonucleotides Source Identifier 
RNF5 gRNA#1: 5′-GCC​CAA​CGA​TCG​TGG​GCA​GG-3′ This paper N/A 
RNF5 gRNA#2: 5′-GAC​TCT​TAC​CTG​TAC​CCC​GG-3′ This paper N/A 
RNF185 gRNA#1: 5′-TGT​AGT​GGT​ACG​GTG​CAA​AC-3′ This paper N/A 
RNF185 gRNA#2: 5′-AAT​GGC​GCT​GGC​GAG​AGC​GG-3′ This paper N/A 
AllStars Neg. Control siRNA (siNT) Qiagen Cat#1027281 
HS_HERC3_4 siRNA: 5′-CAG​CCC​GAG​CTG​ATC​GCT​TTA-3′ Qiagen Cat#SI00107233 
HS_HERC3_5 siRNA: 5′-CAG​CGA​TCG​GAT​TCC​CAT​CTA-3′ Qiagen Cat#SI03066959 
HS_HERC3_8 siRNA: 5′-TTG​AGT​CTC​TAT​ATT​GTC​CAA-3′ Qiagen Cat#SI05092101 
Hs_RNF5_11 siRNA: 5′-CAG​CCA​GAG​AAG​AAT​CAG​TAT-3′ Qiagen Cat#SI05063114 
Hs_RNF5_6 siRNA: 5′-GGG​CGC​GAC​CTT​CGA​ATG​TAA-3′ Qiagen Cat#SI03221484 
Hs_RNF5_7 siRNA: 5′-AAC​GGC​AAG​AGT​GTC​CAG​TAT-3′ Qiagen Cat#SI04204116 
Hs_RNF185_2 siRNA: 5′-CAC​CTT​CTG​GAA​ATC​CGC​AAA-3′ Qiagen Cat#SI03059385 
Hs_RNF185_3 siRNA: 5′-CTC​CGA​GGC​AGT​AAT​AAC​TGA-3′ Qiagen Cat#SI03089982 
Hs_RNF185_8 siRNA: 5′-TCC​CAT​CAG​CTT​TCT​CCC​TAA-3′ Qiagen Cat#SI04959598 
Hs_RFFL_1 siRNA 5′-ATC​GGT​TTC​TTC​AGT​GCC​TTA-3′ Qiagen Cat#SI00148232 
Hs_RFFL_3 siRNA 5′-TCG​CAA​CTT​TGT​CAA​CTA​CAA-3′ Qiagen Cat#SI00148246 
Hs_RFFL_5 siRNA 5′-CTC​CAT​GAC​ATC​TCT​ACC​GAA-3′ Qiagen Cat#SI03089653 
RNF5 genome PCR primer pair: 5′-CCAGCCGAATGAGTTGGGTTAACAC-3′ (FW)
5′-GGCGAGAAACAGGAAGAGGGAATCC-3′ (Rev) 		This paper N/A 
RNF185 genome PCR primer pair: 5′-ACCTAGCAAGCTAGAGGCTG-3′ (FW)
5′-AGGGCTCCTGTAACCGTCAA-3′ (Rev) 		This paper N/A 
M13F sequence primer: 5′-GTA​AAA​CGA​CGG​CCA​GT-3′ This paper N/A 
M13R pUC sequence primer: 5′-CAG​GAA​ACA​GCT​ATG​AC-3′ This paper N/A 
HERC3 qPCR primer pair: 5′-GATCTGCCGAGAAAGCTATGG-3′ (FW)
5′-TTTGCCACCACACACCTTTAG-3′ (Rev) 		This paper N/A 
GAPDH qPCR primer pair: 5′-CATGAGAAGTATGACAACAGCCT-3′ (FW)
5′-AGTCCTTCCACGATACCAAAGT-3′ (Rev) 		This paper N/A 
Software and algorithms Source Identifier 
GraphPad Prism 8 GraphPad Software, Inc RRID:SCR_002798 
Evolution Capt Vilber-Lourmat N/A 
ImageQuant Cytiva RRID:SCR_014246 
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