Table 1.

Cloning of Adm Cas13d nuclease and gRNA plasmids

gRNAs for IGS ncRNAsSite specific sequence (5′ → 3′)Plasmid number
IGS18 5′-GAT​TCC​ACA​AAT​GAA​GGT​CAG​CGG​TAT​CTA-3′ pTP5379 
IGS20 5′-AAA​ACA​TTT​AAA​AAT​GAA​GGC​CGG​GCG​CGG-3′ pTP5372 
IGS22 5′-GTT​CAA​GCA​ATT​CTT​CCA​CCC​CAG​CCT​CCC-3′ pTP5383 
IGS24 5′-TCA​ACC​TAT​GGC​AGA​GAG​GAC​ACG​TCA​TTC-3′ pTP5387 
IGS28 5′-AAC​AGT​TTA​TGT​TGA​AGT​CGA​GGA​GCT​TAT-3′ pTP5371 
IGS30 5′-CAG​AAG​TGA​AGA​ATT​CAG​GCC​TTG​GTA​AGG-3′ pTP5389 
IGS32 5′-ACT​TTC​ACT​ATC​ATT​AGC​ACA​GTG​TTT​AAT-3′ pTP5391 
IGS34 5′-AGT​TTA​TGT​CAC​AGA​CAG​CCC​GAG​ACC​GTT-3′ pTP5392 
IGS36 5′-ACT​ACG​TCT​TAA​CCA​CGC​ACC​CAC​GAA​GAA-3′ pTP5380 
IGS38 5′-TGT​ATT​GTA​TTG​TAT​CGT​ATC​CTA​TTG​TAT-3′ pTP5384 
IGS40 5′-AAG​CAA​GCA​AGA​AAG​CAA​GCA​TGA​AAG​AAA-3′ pTP5385 
gRNAs for IGS ncRNAsSite specific sequence (5′ → 3′)Plasmid number
IGS18 5′-GAT​TCC​ACA​AAT​GAA​GGT​CAG​CGG​TAT​CTA-3′ pTP5379 
IGS20 5′-AAA​ACA​TTT​AAA​AAT​GAA​GGC​CGG​GCG​CGG-3′ pTP5372 
IGS22 5′-GTT​CAA​GCA​ATT​CTT​CCA​CCC​CAG​CCT​CCC-3′ pTP5383 
IGS24 5′-TCA​ACC​TAT​GGC​AGA​GAG​GAC​ACG​TCA​TTC-3′ pTP5387 
IGS28 5′-AAC​AGT​TTA​TGT​TGA​AGT​CGA​GGA​GCT​TAT-3′ pTP5371 
IGS30 5′-CAG​AAG​TGA​AGA​ATT​CAG​GCC​TTG​GTA​AGG-3′ pTP5389 
IGS32 5′-ACT​TTC​ACT​ATC​ATT​AGC​ACA​GTG​TTT​AAT-3′ pTP5391 
IGS34 5′-AGT​TTA​TGT​CAC​AGA​CAG​CCC​GAG​ACC​GTT-3′ pTP5392 
IGS36 5′-ACT​ACG​TCT​TAA​CCA​CGC​ACC​CAC​GAA​GAA-3′ pTP5380 
IGS38 5′-TGT​ATT​GTA​TTG​TAT​CGT​ATC​CTA​TTG​TAT-3′ pTP5384 
IGS40 5′-AAG​CAA​GCA​AGA​AAG​CAA​GCA​TGA​AAG​AAA-3′ pTP5385 
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