Comparison of SRX percentages for all β-cardiac myosin constructs at varying salt concentrations
| KCl . | WT S1 . | WT 2HEP . | WT 15HEP . | WT 25HEP . |
|---|---|---|---|---|
| 20 | 8.7 ± 0.7 | 16.0 ± 6.0 | 89.1 ± 0.8** | 82.5 ± 5.0** |
| 50 | 7.4 ± 0.3 | 8.7 ± 1.9 | 57.4 ± 5.2** | 85.8 ± 8.0**^^ |
| 75 | 7.5 ± 1.0 | 6.0 ± 1.0 | 40.6 ± 7.6** | 77.3 ± 6.4**^^ |
| 100 | 5.7 ± 0.6 | 4.1 ± 2.8 | 15.3 ± 3.3 | 45.4 ± 12.1**^^ |
| 150 | 6.8 ± 0.3 | 2.3 ± 2.0 | 13.0 ± 1.7 | 33.1 ± 15.4**^^ |
| KCl | E525K S1 | E525K 2HEP | E525K 15 HEP | E525K 25HEP |
| 20 | 12.1 ± 3.8 | 8.5 ± 2.3 | 95.0 ± 0.7** | 83.3 ± 0.2**^^ |
| 50 | 6.6 ± 0.3 | 7.1 ± 1.9 | 94.2 ± 0.7** | 81.4 ± 0.2**^^ |
| 75 | 7.1 ± 1.7 | 4.6 ± 1.7 | 91.8 ± 1.6** | 81.3 ± 1.4**^^ |
| 100 | 5.1 ± 0.8 | 3.7 ± 1.1 | 89.6 ± 2.3** | 79.5 ± 1.4**^^ |
| 150 | 4.9 ± 0.2 | 4.4 ± 0.9 | 79.4 ± 3.2** | 78.8 ± 4.0** |
| KCl . | WT S1 . | WT 2HEP . | WT 15HEP . | WT 25HEP . |
|---|---|---|---|---|
| 20 | 8.7 ± 0.7 | 16.0 ± 6.0 | 89.1 ± 0.8** | 82.5 ± 5.0** |
| 50 | 7.4 ± 0.3 | 8.7 ± 1.9 | 57.4 ± 5.2** | 85.8 ± 8.0**^^ |
| 75 | 7.5 ± 1.0 | 6.0 ± 1.0 | 40.6 ± 7.6** | 77.3 ± 6.4**^^ |
| 100 | 5.7 ± 0.6 | 4.1 ± 2.8 | 15.3 ± 3.3 | 45.4 ± 12.1**^^ |
| 150 | 6.8 ± 0.3 | 2.3 ± 2.0 | 13.0 ± 1.7 | 33.1 ± 15.4**^^ |
| KCl | E525K S1 | E525K 2HEP | E525K 15 HEP | E525K 25HEP |
| 20 | 12.1 ± 3.8 | 8.5 ± 2.3 | 95.0 ± 0.7** | 83.3 ± 0.2**^^ |
| 50 | 6.6 ± 0.3 | 7.1 ± 1.9 | 94.2 ± 0.7** | 81.4 ± 0.2**^^ |
| 75 | 7.1 ± 1.7 | 4.6 ± 1.7 | 91.8 ± 1.6** | 81.3 ± 1.4**^^ |
| 100 | 5.1 ± 0.8 | 3.7 ± 1.1 | 89.6 ± 2.3** | 79.5 ± 1.4**^^ |
| 150 | 4.9 ± 0.2 | 4.4 ± 0.9 | 79.4 ± 3.2** | 78.8 ± 4.0** |
No differences S1 versus 2HEP. *P < 0.05 or **P < 0.005 for comparison of S1 versus 15HEP and 25HEP. ^P < 0.05 or ^^P < 0.005 for comparison of 15HEP and 25HEP, see Tables S3 and S4.