Table 2.

Single mantATP turnover measurements for WT and E525K constructs (n = 3 ± SD)

KCl (mM)DRX rate (s−1)SRX rate (s−1)SRX fraction (%)
WT S1 
20 0.034 ± 0.002 0.0020 ± 0.0002 8.7 ± 0.7 
50 0.034 ± 0.001 0.0029 ± 0.0007 7.4 ± 0.3 
75 0.030 ± 0.001 0.0016 ± 0.0001 7.5 ± 1.0 
100 0.028 ± 0.002 0.0029 ± 0.0012 5.7 ± 0.6 
150 0.025 ± 0.001 0.0028 ± 0.0008 6.8 ± 0.3 
E525K S1 
20 0.072 ± 0.005 0.0019 ± 0.0005 12.1 ± 3.8 
50 0.064 ± 0.003 0.0023 ± 0.0003 6.6 ± 0.3 
75 0.063 ± 0.005 0.0017 ± 0.0004 7.1 ± 1.7 
100 0.064 ± 0.006 0.0023 ± 0.0008 5.1 ± 0.8 
150 0.061 ± 0.001 0.0024 ± 0.0005 4.9 ± 0.2** 
KCl (mM) DRX rate (s−1) SRX rate (s−1) SRX fraction (%) 
WT 2HEP 
20 0.022 ± 0.001 0.0066 ± 0.0017 16.0 ± 6.0 
50 0.025 ± 0.001 0.0053 ± 0.0006 8.7 ± 1.9 
75 0.028 ± 0.001 0.0047 ± 0.0031 6.0 ± 1.0 
100 0.030 ± 0.001 0.0047 ± 0.0027 4.1 ± 2.8 
150 0.031 ± 0.002 0.0038 ± 0.0036 2.3 ± 2.0 
E525K 2HEP 
20 0.045 ± 0.002 0.0033 ± 0.0005 8.5 ± 2.3 
50 0.059 ± 0.002 0.0073 ± 0.0030 7.1 ± 1.9 
75 0.063 ± 0.003 0.0052 ± 0.0013 4.6 ± 1.7 
100 0.062 ± 0.001 0.0040 ± 0.0018 3.7 ± 1.1 
150 0.068 ± 0.001 0.0042 ± 0.0046 4.4 ± 0.9 
KCl (mM) DRX rate (s−1) SRX rate (s−1) SRX fraction (%) 
WT 15HEP 
20 0.014 ± 0.002 0.0033 ± 0.0001 89.1 ± 0.8 
50 0.013 ± 0.001 0.0054 ± 0.0005 57.4 ± 5.2 
75 0.015 ± 0.002 0.0069 ± 0.0009 40.6 ± 7.6 
100 0.016 ± 0.001 0.0058 ± 0.0004 15.3 ± 3.3 
150 0.018 ± 0.001 0.0071 ± 0.0008 13.0 ± 1.7 
E525K 15HEP 
20 0.027 ± 0.008 0.0022 ± 0.0002 95.0 ± 0.7** 
50 0.019 ± 0.002 0.0026 ± 0.0001 94.2 ± 0.7** 
75 0.016 ± 0.006 0.0030 ± 0.0001 91.8 ± 1.6** 
100 0.014 ± 0.003 0.0033 ± 0.0001 89.6 ± 2.3** 
150 0.013 ± 0.002 0.0042 ± 0.0001 79.4 ± 3.2** 
KCl (mM) DRX rate (s−1) SRX rate (s−1) SRX fraction (%) 
WT 25HEP 
20 0.036 ± 0.009 0.0030 ± 0.0001 82.5 ± 5.0 
50 0.033 ± 0.016 0.0039 ± 0.0004 85.8 ± 8.0 
75 0.025 ± 0.012 0.0039 ± 0.0004 77.3 ± 6.4 
100 0.011 ± 0.003 0.0029 ± 0.0005 45.4 ± 12.1 
150 0.010 ± 0.001 0.0032 ± 0.0008 33.1 ± 15.4 
E525K 25HEP 
20 0.029 ± 0.003 0.0030 ± 0.0001 83.3 ± 0.2 
50 0.027 ± 0.006 0.0034 ± 0.0002 81.4 ± 0.2 
75 0.027 ± 0.004 0.0041 ± 0.0006 81.3 ± 1.4 
100 0.034 ± 0.003 0.0048 ± 0.0002 79.5 ± 1.4* 
150 0.039 ± 0.011 0.0061 ± 0.0002 78.8 ± 4.0* 
KCl (mM)DRX rate (s−1)SRX rate (s−1)SRX fraction (%)
WT S1 
20 0.034 ± 0.002 0.0020 ± 0.0002 8.7 ± 0.7 
50 0.034 ± 0.001 0.0029 ± 0.0007 7.4 ± 0.3 
75 0.030 ± 0.001 0.0016 ± 0.0001 7.5 ± 1.0 
100 0.028 ± 0.002 0.0029 ± 0.0012 5.7 ± 0.6 
150 0.025 ± 0.001 0.0028 ± 0.0008 6.8 ± 0.3 
E525K S1 
20 0.072 ± 0.005 0.0019 ± 0.0005 12.1 ± 3.8 
50 0.064 ± 0.003 0.0023 ± 0.0003 6.6 ± 0.3 
75 0.063 ± 0.005 0.0017 ± 0.0004 7.1 ± 1.7 
100 0.064 ± 0.006 0.0023 ± 0.0008 5.1 ± 0.8 
150 0.061 ± 0.001 0.0024 ± 0.0005 4.9 ± 0.2** 
KCl (mM) DRX rate (s−1) SRX rate (s−1) SRX fraction (%) 
WT 2HEP 
20 0.022 ± 0.001 0.0066 ± 0.0017 16.0 ± 6.0 
50 0.025 ± 0.001 0.0053 ± 0.0006 8.7 ± 1.9 
75 0.028 ± 0.001 0.0047 ± 0.0031 6.0 ± 1.0 
100 0.030 ± 0.001 0.0047 ± 0.0027 4.1 ± 2.8 
150 0.031 ± 0.002 0.0038 ± 0.0036 2.3 ± 2.0 
E525K 2HEP 
20 0.045 ± 0.002 0.0033 ± 0.0005 8.5 ± 2.3 
50 0.059 ± 0.002 0.0073 ± 0.0030 7.1 ± 1.9 
75 0.063 ± 0.003 0.0052 ± 0.0013 4.6 ± 1.7 
100 0.062 ± 0.001 0.0040 ± 0.0018 3.7 ± 1.1 
150 0.068 ± 0.001 0.0042 ± 0.0046 4.4 ± 0.9 
KCl (mM) DRX rate (s−1) SRX rate (s−1) SRX fraction (%) 
WT 15HEP 
20 0.014 ± 0.002 0.0033 ± 0.0001 89.1 ± 0.8 
50 0.013 ± 0.001 0.0054 ± 0.0005 57.4 ± 5.2 
75 0.015 ± 0.002 0.0069 ± 0.0009 40.6 ± 7.6 
100 0.016 ± 0.001 0.0058 ± 0.0004 15.3 ± 3.3 
150 0.018 ± 0.001 0.0071 ± 0.0008 13.0 ± 1.7 
E525K 15HEP 
20 0.027 ± 0.008 0.0022 ± 0.0002 95.0 ± 0.7** 
50 0.019 ± 0.002 0.0026 ± 0.0001 94.2 ± 0.7** 
75 0.016 ± 0.006 0.0030 ± 0.0001 91.8 ± 1.6** 
100 0.014 ± 0.003 0.0033 ± 0.0001 89.6 ± 2.3** 
150 0.013 ± 0.002 0.0042 ± 0.0001 79.4 ± 3.2** 
KCl (mM) DRX rate (s−1) SRX rate (s−1) SRX fraction (%) 
WT 25HEP 
20 0.036 ± 0.009 0.0030 ± 0.0001 82.5 ± 5.0 
50 0.033 ± 0.016 0.0039 ± 0.0004 85.8 ± 8.0 
75 0.025 ± 0.012 0.0039 ± 0.0004 77.3 ± 6.4 
100 0.011 ± 0.003 0.0029 ± 0.0005 45.4 ± 12.1 
150 0.010 ± 0.001 0.0032 ± 0.0008 33.1 ± 15.4 
E525K 25HEP 
20 0.029 ± 0.003 0.0030 ± 0.0001 83.3 ± 0.2 
50 0.027 ± 0.006 0.0034 ± 0.0002 81.4 ± 0.2 
75 0.027 ± 0.004 0.0041 ± 0.0006 81.3 ± 1.4 
100 0.034 ± 0.003 0.0048 ± 0.0002 79.5 ± 1.4* 
150 0.039 ± 0.011 0.0061 ± 0.0002 78.8 ± 4.0* 

*P < 0.05 for comparison of E525K and WT, see Tables S3 and S4. **P < 0.005 for comparison of E525K and WT, see Tables S3 and S4.

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