Table 1. Voltage-dependent activation... | Rockefeller University Press

Table 1.

Voltage-dependent activation parameters

	Pre-treatment				Post-treatment
	V_1/2 (mV)	n	s	n	V_1/2 (mV)	n	s	n	P value (V_1/2)
Holo HCN4
+ Vehicle	−102.3 ± 2.0	7	8.9 ± 1.7	7	−104.1 ± 2.9	7	9.0 ± 0.69	7	0.64
+1 µM CBD	−102.5 ± 1.4	6	9.6 ± 0.64	6	−98.8 ± 1.8	6	10.3 ± 0.77	6	0.17
+2.5 µM CBD	−105.4 ± 2.5	5	9.8 ± 1.1	5	−95.0 ± 2.7	5	10.2 ± 0.72	5	0.022
+5 µM CBD	−102.1 ± 1.1	9	9.8 ± 0.55	9	−87.5 ± 3.0	9	9.8 ± 0.99	9	0.0002
+10 µM CBD	−98.2 ± 2.2	6	9.4 ± 1.3	6	−83.4 ± 1.5	6	11.3 ± 1.9	6	0.0005
+20 µM CBD	−102.7 ± 1.6	11	9.5 ± 0.8	11	−89.3 ± 2.1	11	10.2 ± 0.69	11	<0.0001
+10 µM diC8-PIP₂; +5 µM CBD					−76.5 ± 3.4	5	7.9 ± 0.75	5	n/a
Apo HCN4
+5 µM CBD	−113.2 ± 2.2	7	13.5 ± 1.5	7	−97.6 ± 2.5	7	8.7 ± 0.79	7	0.0005

	Pre-treatment				Post-treatment
	V_1/2 (mV)	n	s	n	V_1/2 (mV)	n	s	n	P value (V_1/2)
Holo HCN4
+ Vehicle	−102.3 ± 2.0	7	8.9 ± 1.7	7	−104.1 ± 2.9	7	9.0 ± 0.69	7	0.64
+1 µM CBD	−102.5 ± 1.4	6	9.6 ± 0.64	6	−98.8 ± 1.8	6	10.3 ± 0.77	6	0.17
+2.5 µM CBD	−105.4 ± 2.5	5	9.8 ± 1.1	5	−95.0 ± 2.7	5	10.2 ± 0.72	5	0.022
+5 µM CBD	−102.1 ± 1.1	9	9.8 ± 0.55	9	−87.5 ± 3.0	9	9.8 ± 0.99	9	0.0002
+10 µM CBD	−98.2 ± 2.2	6	9.4 ± 1.3	6	−83.4 ± 1.5	6	11.3 ± 1.9	6	0.0005
+20 µM CBD	−102.7 ± 1.6	11	9.5 ± 0.8	11	−89.3 ± 2.1	11	10.2 ± 0.69	11	<0.0001
+10 µM diC8-PIP₂; +5 µM CBD					−76.5 ± 3.4	5	7.9 ± 0.75	5	n/a
Apo HCN4
+5 µM CBD	−113.2 ± 2.2	7	13.5 ± 1.5	7	−97.6 ± 2.5	7	8.7 ± 0.79	7	0.0005

Average ± S.E.M. for V_1/2 and slope values calculated from the Boltzmann function. P value given from paired t test performed for each paired condition (i.e., CBD concentration or vehicle).