Table 1.

Comparison of CD19/CD3-bispecific TCE and autologous CD19 CAR-T cells

FeatureCD19/CD3-bispecific TCEAutologous CD19 CAR-T cell therapy
Manufacturing • Standard antibody manufacturing
• Off-the-shelf availability 
• Individualized CAR-T cell production; delayed treatment
• High cost and complexity 
Mode of action • Can potentially engage all T cells in a patient for redirected target cell lysis
• Potential to eliminate cells expressing very low levels of CD19 
• Redirected target cell lysis relies on limited number of genetically engineered T cells 
PK/pharmacodynamics (PD) • Antibody construct with well-defined PK/PD properties • “Living drug” with difficult-to-predict PK/PD 
Administration • IV or SC administration with potential for self-administration
• Ability to re-treat if required 
• Onetime treatment
• IV route only 
Safety • CRS and ICANS
• SC administration and step-up dosing have potential to limit CRS and ICANS to grade 1 or 2 
• Risk for grade 3 and higher CRS and ICANS
• Adverse events from lymphodepletion
• Risk of secondary T cell malignancies 
Experience with modality • Eight FDA-approved TCEs (five targets) • Six FDA-approved CAR-T cell products (two targets) 
FeatureCD19/CD3-bispecific TCEAutologous CD19 CAR-T cell therapy
Manufacturing • Standard antibody manufacturing
• Off-the-shelf availability 
• Individualized CAR-T cell production; delayed treatment
• High cost and complexity 
Mode of action • Can potentially engage all T cells in a patient for redirected target cell lysis
• Potential to eliminate cells expressing very low levels of CD19 
• Redirected target cell lysis relies on limited number of genetically engineered T cells 
PK/pharmacodynamics (PD) • Antibody construct with well-defined PK/PD properties • “Living drug” with difficult-to-predict PK/PD 
Administration • IV or SC administration with potential for self-administration
• Ability to re-treat if required 
• Onetime treatment
• IV route only 
Safety • CRS and ICANS
• SC administration and step-up dosing have potential to limit CRS and ICANS to grade 1 or 2 
• Risk for grade 3 and higher CRS and ICANS
• Adverse events from lymphodepletion
• Risk of secondary T cell malignancies 
Experience with modality • Eight FDA-approved TCEs (five targets) • Six FDA-approved CAR-T cell products (two targets) 

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