Table 1.

Amino acid sequences of the selectivity filters of different NaV and CaV channels

ChannelLocus EEEELocus DCS
HsCaV1.1 EEEE DSQE 
HsCaV1.2 EEEE DSED 
HsCaV2.1 EEEE DEQN 
HsCaV2.2 EEEE DAME 
HsCaV2.3 EEEE TEQE 
HsCaV3.1 EEDD DVNG 
Am-CaVDEEA DEED 
DSC1 DEEA NEED 
BSC1 DEEA NEED 
Am-NaV DEKA NEQG 
ChannelLocus EEEELocus DCS
HsCaV1.1 EEEE DSQE 
HsCaV1.2 EEEE DSED 
HsCaV2.1 EEEE DEQN 
HsCaV2.2 EEEE DAME 
HsCaV2.3 EEEE TEQE 
HsCaV3.1 EEDD DVNG 
Am-CaVDEEA DEED 
DSC1 DEEA NEED 
BSC1 DEEA NEED 
Am-NaV DEKA NEQG 

The EEEE locus is the locus responsible for divalent/monovalent selectivity, while the DCS (divalent cation selectivity) is the locus responsible for the selectivity between divalent cations (Ba2+, Ca2+, Sr2+) (Cens et al., 2007). For CaV4, Am-NaV, DSC1, and BSC1 the location of the DCS locus is taken relative to the conserved tryptophan in each pore domain.

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