Reagents and resources used in this study
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| IgA, PE | Invitrogen | Cat#12-4204-81 |
| IgG, Alexa Fluor 594 | BioLegend | Cat#405326 |
| CD45, Violet 450 | Biogems | Cat#07512-40-108 |
| CD4, Alexa Fluor 647 | BioLegend | Cat#100412 |
| TCR β chain, FITC | BioLegend | Cat#109206 |
| IgG1 κ Isotype Ctrl, PE | BioLegend | Cat#400407 |
| ROR gamma (t) | Invitrogen | Cat#12-69981-80 |
| IFNg, APC | BioLegend | Cat#505810 |
| IL17, PE | BioLegend | Cat#506904 |
| TCR β chain, PE/Cyanine5 | BioLegend | Cat#109209 |
| CD274, PE/Dazzle 594 | BioLegend | Cat#124323 |
| CD43, Alexa Fluor 700 | BioLegend | Cat#143214 |
| Ly6C, Pacific Blue | BioLegend | Cat#128014 |
| Ly6G, FITC | BioLegend | Cat#127605 |
| CD115, APC/Cyanine7 | BioLegend | Cat#135532 |
| CD11b, PE | BioLegend | Cat#101207 |
| CD220, APC | BioLegend | Cat#103212 |
| Zombie Aqua Fixable Viability Kit | BioLegend | Cat#423102 |
| purified CD16/32 | BioLegend | Cat#101302 |
| Cell Activation Cocktail | BioLegend | Cat#423302 |
| Brefeldin A Solution | BioLegend | Cat#420601 |
| Fixation/Permeablization Kit | BD Bioscience | Cat# 554714 |
| True-Nuclear TF Buffer Set | BioLegend | Cat#424401 |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| IgA, PE | Invitrogen | Cat#12-4204-81 |
| IgG, Alexa Fluor 594 | BioLegend | Cat#405326 |
| CD45, Violet 450 | Biogems | Cat#07512-40-108 |
| CD4, Alexa Fluor 647 | BioLegend | Cat#100412 |
| TCR β chain, FITC | BioLegend | Cat#109206 |
| IgG1 κ Isotype Ctrl, PE | BioLegend | Cat#400407 |
| ROR gamma (t) | Invitrogen | Cat#12-69981-80 |
| IFNg, APC | BioLegend | Cat#505810 |
| IL17, PE | BioLegend | Cat#506904 |
| TCR β chain, PE/Cyanine5 | BioLegend | Cat#109209 |
| CD274, PE/Dazzle 594 | BioLegend | Cat#124323 |
| CD43, Alexa Fluor 700 | BioLegend | Cat#143214 |
| Ly6C, Pacific Blue | BioLegend | Cat#128014 |
| Ly6G, FITC | BioLegend | Cat#127605 |
| CD115, APC/Cyanine7 | BioLegend | Cat#135532 |
| CD11b, PE | BioLegend | Cat#101207 |
| CD220, APC | BioLegend | Cat#103212 |
| Zombie Aqua Fixable Viability Kit | BioLegend | Cat#423102 |
| purified CD16/32 | BioLegend | Cat#101302 |
| Cell Activation Cocktail | BioLegend | Cat#423302 |
| Brefeldin A Solution | BioLegend | Cat#420601 |
| Fixation/Permeablization Kit | BD Bioscience | Cat# 554714 |
| True-Nuclear TF Buffer Set | BioLegend | Cat#424401 |