Model-independent descriptive parameters for the indicated mutant z/hCFTR constructs
| . | τburst (s) . | τopen (ms) . | τflicker (ms) . | Keq|B . |
|---|---|---|---|---|
| zE1372S | 57 ± 8.3 (9) | 19 ± 3.6 | 55 ± 8.0 | 0.35 ± 0.079 (12) |
| zE1372Q | 78 ± 6.7 (6) | n.d. | n.d. | n.d. |
| zS109A | 19 ± 3.0 (7) | 14 ± 3.3 | 150 ± 32 | 0.094 ± 0.027 (9) |
| zN120A | 40 ± 7.7 (6) | 37 ± 6.2 | 14 ± 1.5 | 2.6 ± 0.51 (5) |
| zS109A-N120A | 13 ± 0.82 (7) | 9.0 ± 1.5 | 200 ± 31 | 0.045 ± 0.0094 (12) |
| zR118H | 72 ± 11 (7) | n.d. | n.d. | n.d. |
| zS109A-R118H | 36 ± 3.4 (7) | n.d. | n.d. | n.d. |
| hE1371S | 37 ± 3.7 (7) | 200 ± 23 | 10 ± 2.2 | 19 ± 4.1 (6) |
| hE1371Q | 470 ± 65 (4) | n.d. | n.d. | n.d. |
| hS108A | 11 ± 1.4 (9) | 33 ± 2.8 | 120 ± 16 | 0.27 ± 0.041 (7) |
| hI119N | 50 ± 7.9 (8) | 66 ± 5.8 | 61 ± 8.6 | 1.1 ± 0.17 (9) |
| hS108A-I119N | 29 ± 5.0 (8) | 19 ± 3.6 | 260 ± 39 | 0.074 ± 0.017 (7) |
| hR117H | 9.5 ± 0.73 (8) | 11 ± 2.1 | 150 ± 24 | 0.072 ± 0.018 (8) |
| hS108A-R117H | 19 ± 2.4 (9) | 5.4 ± 1.5 | 300 ± 38 | 0.018 ± 0.0054 (6) |
| . | τburst (s) . | τopen (ms) . | τflicker (ms) . | Keq|B . |
|---|---|---|---|---|
| zE1372S | 57 ± 8.3 (9) | 19 ± 3.6 | 55 ± 8.0 | 0.35 ± 0.079 (12) |
| zE1372Q | 78 ± 6.7 (6) | n.d. | n.d. | n.d. |
| zS109A | 19 ± 3.0 (7) | 14 ± 3.3 | 150 ± 32 | 0.094 ± 0.027 (9) |
| zN120A | 40 ± 7.7 (6) | 37 ± 6.2 | 14 ± 1.5 | 2.6 ± 0.51 (5) |
| zS109A-N120A | 13 ± 0.82 (7) | 9.0 ± 1.5 | 200 ± 31 | 0.045 ± 0.0094 (12) |
| zR118H | 72 ± 11 (7) | n.d. | n.d. | n.d. |
| zS109A-R118H | 36 ± 3.4 (7) | n.d. | n.d. | n.d. |
| hE1371S | 37 ± 3.7 (7) | 200 ± 23 | 10 ± 2.2 | 19 ± 4.1 (6) |
| hE1371Q | 470 ± 65 (4) | n.d. | n.d. | n.d. |
| hS108A | 11 ± 1.4 (9) | 33 ± 2.8 | 120 ± 16 | 0.27 ± 0.041 (7) |
| hI119N | 50 ± 7.9 (8) | 66 ± 5.8 | 61 ± 8.6 | 1.1 ± 0.17 (9) |
| hS108A-I119N | 29 ± 5.0 (8) | 19 ± 3.6 | 260 ± 39 | 0.074 ± 0.017 (7) |
| hR117H | 9.5 ± 0.73 (8) | 11 ± 2.1 | 150 ± 24 | 0.072 ± 0.018 (8) |
| hS108A-R117H | 19 ± 2.4 (9) | 5.4 ± 1.5 | 300 ± 38 | 0.018 ± 0.0054 (6) |
Gating parameters were obtained from macroscopic current relaxation and last open-channel recordings. Data are displayed as mean ± SEM, and numbers of independent biological replicates are given in parentheses (for τopen and τflicker, they are the same as for Keq|B).