Table 1.

Primers used for genotyping by PCR

GeneForwardReverseAnnealing temperature
TBK1-E696K floxed 5′-GCC​TCC​GGC​GGC​GTC​AAG-3′ 5′-AAC​CAC​GCC​TTC​CAT​CTC​C-3′ 65.3°C 
TBK1-E696K 5′-GCC​TCC​GGC​GGC​GTC​AAG-3′ 5′-CAT​CAC​TAC​TCT​TCT​G-3′ 65.3°C 
Cre 5′-GCG​GTC​TGG​CAC​TAT​C-3′ 5′-GTG​AAA​CAG​CTC​ACT​T-3′ 51.7°C 
GeneForwardReverseAnnealing temperature
TBK1-E696K floxed 5′-GCC​TCC​GGC​GGC​GTC​AAG-3′ 5′-AAC​CAC​GCC​TTC​CAT​CTC​C-3′ 65.3°C 
TBK1-E696K 5′-GCC​TCC​GGC​GGC​GTC​AAG-3′ 5′-CAT​CAC​TAC​TCT​TCT​G-3′ 65.3°C 
Cre 5′-GCG​GTC​TGG​CAC​TAT​C-3′ 5′-GTG​AAA​CAG​CTC​ACT​T-3′ 51.7°C 
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