In vitro diaphragm muscle mechanics—long-term tirasemtiv treatment
| Frequency (Hz) . | 1 . | 5 . | 10 . | 20 . | 40 . | 60 . | 80 . | 100 . | 150 . | # of mice . | Age of mice in months . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acta1(Wt)—vehicle | 7 | 9 | |||||||||
| Absolute force (mN) | 20 ± 8 | 22 ± 8 | 26 ± 10 | 41 ± 18 | 62 ± 24 | 71 ± 26 | 75 ± 26 | 76 ± 26 | 77 ± 26 | ||
| Normalized force (mN/mm2) | 40 ± 16 | 44 ± 16 | 51 ± 19 | 82 ± 38 | 122 ± 46 | 140 ± 46 | 147 ± 45 | 149 ± 45 | 150 ± 46 | ||
| Relative force (% of maximum) | 27 ± 6 | 29 ± 7 | 34 ± 9 | 54 ± 16 | 80 ± 12 | 93 ± 8 | 98 ± 5 | 100 ± 3 | 100 ± 0.0 | ||
| Acta1(Wt)—tirasemtiva | 8 | 9 | |||||||||
| Absolute force (mN) | 24 ± 8 | 28 ± 11 | 34 ± 15 | 57 ± 20 | 81 ± 24 | 91 ± 25 | 95 ± 26 | 96 ± 26 | 97 ± 27 | ||
| Normalized force (mN/mm2) | 44 ± 16 | 51 ± 20 | 63 ± 26 | 105 ± 38 | 148 ± 44 | 165 ± 48 | 172 ± 44 | 174 ± 44 | 176 ± 44 | ||
| Relative force (% of maximum) | 25 ± 6 | 29 ± 6 | 36 ± 13 | 58 ± 14 | 84 ± 9 | 93 ± 5 | 97 ± 3 | 99 ± 2 | 100 ± 0.0 | ||
| Acta1(D286G)—vehicleb | 15 | 9 | |||||||||
| Absolute force (mN) | 21 ± 8 | 20 ± 8 | 22 ± 9 | 29 ± 15 | 47 ± 21 | 56 ± 23 | 60 ± 23 | 62 ± 23 | 64 ± 23 | ||
| Normalized force (mN/mm2) | 65 ± 26 | 59 ± 26 | 65 ± 28 | 84 ± 37 | 133 ± 49 | 159 ± 51 | 171 ± 50 | 177 ± 50 | 181 ± 51 | ||
| Relative force (% of maximum) | 30 ± 9 | 33 ± 9 | 35 ± 9 | 45 ± 11 | 73 ± 14 | 87 ± 11 | 95 ± 5 | 98 ± 2 | 100 ± 0.0 | ||
| Acta1(D286G)—tirasemtiv | 14 | 9 | |||||||||
| Absolute force (mN) | 17 ± 7 | 20 ± 11 | 25 ± 16 | 33 ± 24 | 47 ± 27 | 54 ± 29 | 58 ± 30 | 60 ± 30 | 61 ± 31 | ||
| Normalized force (mN/mm2) | 51 ± 23 | 61 ± 19 | 75 ± 41 | 98 ± 70 | 140 ± 78 | 162 ± 82 | 174 ± 85 | 178 ± 86 | 183 ± 88 | ||
| Relative force (% of maximum) | 29 ± 8 | 34 ± 9 | 41 ± 12 | 51 ± 19 | 76 ± 13 | 89 ± 10 | 95 ± 6 | 98 ± 3 | 100 ± 0.0 | ||
| Frequency (Hz) . | 1 . | 5 . | 10 . | 20 . | 40 . | 60 . | 80 . | 100 . | 150 . | # of mice . | Age of mice in months . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acta1(Wt)—vehicle | 7 | 9 | |||||||||
| Absolute force (mN) | 20 ± 8 | 22 ± 8 | 26 ± 10 | 41 ± 18 | 62 ± 24 | 71 ± 26 | 75 ± 26 | 76 ± 26 | 77 ± 26 | ||
| Normalized force (mN/mm2) | 40 ± 16 | 44 ± 16 | 51 ± 19 | 82 ± 38 | 122 ± 46 | 140 ± 46 | 147 ± 45 | 149 ± 45 | 150 ± 46 | ||
| Relative force (% of maximum) | 27 ± 6 | 29 ± 7 | 34 ± 9 | 54 ± 16 | 80 ± 12 | 93 ± 8 | 98 ± 5 | 100 ± 3 | 100 ± 0.0 | ||
| Acta1(Wt)—tirasemtiva | 8 | 9 | |||||||||
| Absolute force (mN) | 24 ± 8 | 28 ± 11 | 34 ± 15 | 57 ± 20 | 81 ± 24 | 91 ± 25 | 95 ± 26 | 96 ± 26 | 97 ± 27 | ||
| Normalized force (mN/mm2) | 44 ± 16 | 51 ± 20 | 63 ± 26 | 105 ± 38 | 148 ± 44 | 165 ± 48 | 172 ± 44 | 174 ± 44 | 176 ± 44 | ||
| Relative force (% of maximum) | 25 ± 6 | 29 ± 6 | 36 ± 13 | 58 ± 14 | 84 ± 9 | 93 ± 5 | 97 ± 3 | 99 ± 2 | 100 ± 0.0 | ||
| Acta1(D286G)—vehicleb | 15 | 9 | |||||||||
| Absolute force (mN) | 21 ± 8 | 20 ± 8 | 22 ± 9 | 29 ± 15 | 47 ± 21 | 56 ± 23 | 60 ± 23 | 62 ± 23 | 64 ± 23 | ||
| Normalized force (mN/mm2) | 65 ± 26 | 59 ± 26 | 65 ± 28 | 84 ± 37 | 133 ± 49 | 159 ± 51 | 171 ± 50 | 177 ± 50 | 181 ± 51 | ||
| Relative force (% of maximum) | 30 ± 9 | 33 ± 9 | 35 ± 9 | 45 ± 11 | 73 ± 14 | 87 ± 11 | 95 ± 5 | 98 ± 2 | 100 ± 0.0 | ||
| Acta1(D286G)—tirasemtiv | 14 | 9 | |||||||||
| Absolute force (mN) | 17 ± 7 | 20 ± 11 | 25 ± 16 | 33 ± 24 | 47 ± 27 | 54 ± 29 | 58 ± 30 | 60 ± 30 | 61 ± 31 | ||
| Normalized force (mN/mm2) | 51 ± 23 | 61 ± 19 | 75 ± 41 | 98 ± 70 | 140 ± 78 | 162 ± 82 | 174 ± 85 | 178 ± 86 | 183 ± 88 | ||
| Relative force (% of maximum) | 29 ± 8 | 34 ± 9 | 41 ± 12 | 51 ± 19 | 76 ± 13 | 89 ± 10 | 95 ± 6 | 98 ± 3 | 100 ± 0.0 | ||