In vitro diaphragm muscle mechanics—acute tirasemtiv treatment
| Frequency (Hz) . | 1 . | 5 . | 10 . | 20 . | 40 . | 60 . | 80 . | 100 . | 150 . | # of mice . | Age of mice in months . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acta1(Wt) vehicle | 9 | 8 | |||||||||
| Absolute force (mN) | 20 ± 8 | 21 ± 9 | 23 ± 10 | 32 ± 16 | 59 ± 23 | 71 ± 26 | 76 ± 27 | 80 ± 28 | 82 ± 28 | ||
| Normalized force (mN/mm2) | 32 ± 10 | 33 ± 10 | 36 ± 12 | 51 ± 22 | 94 ± 32 | 113 ± 35 | 122 ± 36 | 128 ± 37 | 131 ± 39 | ||
| Relative force (% of maximum) | 24 ± 5 | 26 ± 5 | 28 ± 6 | 38 ± 10 | 71 ± 9 | 86 ± 6 | 93 ± 3 | 98 ± 2 | 99 ± 1 | ||
| Acta1(Wt)—tirasemtiv | 9 | 8 | |||||||||
| Absolute force (mN) | 14 ± 7 | 18 ± 80 | 26 ± 10 | 42 ± 18 | 61 ± 27 | 69 ± 30 | 73 ± 32 | 75 ± 32 | 76 ± 33 | ||
| Normalized force (mN/mm2) | 24 ± 10a | 31 ± 12 | 46 ± 14a | 73 ± 24a | 104 ± 33a | 117 ± 35 | 123 ± 36 | 127 ± 36 | 129 ± 36 | ||
| Relative force (% of maximum) | 18a ± 5 | 24 ± 5 | 35a ± 8 | 56a ± 11a | 79a ± 10 | 90a ± 7 | 95 ± 4 | 98 ± 2 | 100 ± 0.2 | ||
| Acta1(D286G)—vehicle | 10 | 8 | |||||||||
| Absolute force (mN) | 15 ± 7 | 16 ± 7 | 18 ± 9 | 24 ± 13 | 50 ± 24 | 63 ± 24 | 69 ± 24 | 72 ± 25 | 75 ± 25 | ||
| Normalized force (mN/mm2) | 31 ± 10 | 30 ± 11 | 34 ± 14 | 46 ± 23 | 95 ± 44 | 120 ± 47 | 133 ± 49 | 140 ± 51 | 145 ± 52 | ||
| Relative force (% of maximum) | 20 ± 7 | 21 ± 7 | 34 ± 8 | 32 ± 12 | 65 ± 16 | 83 ± 10 | 92 ± 7 | 96 ± 3 | 100 ± 0.07 | ||
| Acta1(D286G)—tirasemtivb | 10 | 8 | |||||||||
| Absolute force (mN) | 11 ± 6 | 14 ± 6 | 21 ± 9 | 35 ± 15 | 52 ± 22 | 62 ± 24 | 64 ± 24 | 65 ± 25 | 67 ± 25 | ||
| Normalized force (mN/mm2) | 24 ± 8 | 31 ± 8 | 48 ± 12 | 79 ± 19 | 116 ± 27 | 134 ± 27 | 14 ± 26 | 146 ± 26 | 149 ± 26 | ||
| Relative force (% of maximum) | 16 ± 3a | 21 ± 4 | 32 ± 6a | 53 ± 9a | 78 ± 8 | 90 ± 4 | 96 ± 2 | 98 ± 1 | 100 ± 0.0 | ||
| Frequency (Hz) . | 1 . | 5 . | 10 . | 20 . | 40 . | 60 . | 80 . | 100 . | 150 . | # of mice . | Age of mice in months . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Acta1(Wt) vehicle | 9 | 8 | |||||||||
| Absolute force (mN) | 20 ± 8 | 21 ± 9 | 23 ± 10 | 32 ± 16 | 59 ± 23 | 71 ± 26 | 76 ± 27 | 80 ± 28 | 82 ± 28 | ||
| Normalized force (mN/mm2) | 32 ± 10 | 33 ± 10 | 36 ± 12 | 51 ± 22 | 94 ± 32 | 113 ± 35 | 122 ± 36 | 128 ± 37 | 131 ± 39 | ||
| Relative force (% of maximum) | 24 ± 5 | 26 ± 5 | 28 ± 6 | 38 ± 10 | 71 ± 9 | 86 ± 6 | 93 ± 3 | 98 ± 2 | 99 ± 1 | ||
| Acta1(Wt)—tirasemtiv | 9 | 8 | |||||||||
| Absolute force (mN) | 14 ± 7 | 18 ± 80 | 26 ± 10 | 42 ± 18 | 61 ± 27 | 69 ± 30 | 73 ± 32 | 75 ± 32 | 76 ± 33 | ||
| Normalized force (mN/mm2) | 24 ± 10a | 31 ± 12 | 46 ± 14a | 73 ± 24a | 104 ± 33a | 117 ± 35 | 123 ± 36 | 127 ± 36 | 129 ± 36 | ||
| Relative force (% of maximum) | 18a ± 5 | 24 ± 5 | 35a ± 8 | 56a ± 11a | 79a ± 10 | 90a ± 7 | 95 ± 4 | 98 ± 2 | 100 ± 0.2 | ||
| Acta1(D286G)—vehicle | 10 | 8 | |||||||||
| Absolute force (mN) | 15 ± 7 | 16 ± 7 | 18 ± 9 | 24 ± 13 | 50 ± 24 | 63 ± 24 | 69 ± 24 | 72 ± 25 | 75 ± 25 | ||
| Normalized force (mN/mm2) | 31 ± 10 | 30 ± 11 | 34 ± 14 | 46 ± 23 | 95 ± 44 | 120 ± 47 | 133 ± 49 | 140 ± 51 | 145 ± 52 | ||
| Relative force (% of maximum) | 20 ± 7 | 21 ± 7 | 34 ± 8 | 32 ± 12 | 65 ± 16 | 83 ± 10 | 92 ± 7 | 96 ± 3 | 100 ± 0.07 | ||
| Acta1(D286G)—tirasemtivb | 10 | 8 | |||||||||
| Absolute force (mN) | 11 ± 6 | 14 ± 6 | 21 ± 9 | 35 ± 15 | 52 ± 22 | 62 ± 24 | 64 ± 24 | 65 ± 25 | 67 ± 25 | ||
| Normalized force (mN/mm2) | 24 ± 8 | 31 ± 8 | 48 ± 12 | 79 ± 19 | 116 ± 27 | 134 ± 27 | 14 ± 26 | 146 ± 26 | 149 ± 26 | ||
| Relative force (% of maximum) | 16 ± 3a | 21 ± 4 | 32 ± 6a | 53 ± 9a | 78 ± 8 | 90 ± 4 | 96 ± 2 | 98 ± 1 | 100 ± 0.0 | ||