In vitro EDL muscle mechanics—long-term tirasemtiv treatment
| Frequency (Hz) . | 1 . | 5 . | 10 . | 20 . | 40 . | 60 . | 80 . | 100 . | 150 . | 200 . | # of mice . | Age of mice in months . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Acta1(Wt)—vehicle | 7 | 9 | ||||||||||
| Absolute force (mN) | 52 ± 22 | 50 ± 21 | 49 ± 20 | 49 ± 21 | 92 ± 48 | 140 ± 73 | 178 ± 89 | 204 ± 95 | 237 ± 98 | 248 ± 95 | ||
| Normalized force (mN/mm2) | 72 ± 30 | 68 ± 28 | 67 ± 28 | 67 ± 28 | 127 ± 57 | 192 ± 100 | 244 ± 122 | 280 ± 130 | 325 ± 134 | 340 ± 130 | ||
| Relative force (% of maximum) | 21 ± 2 | 20 ± 2 | 19 ± 2 | 19 ± 2 | 34 ± 7 | 53 ± 9 | 69 ± 8 | 81 ± 6 | 95 ± 3 | 100 ± 0.0 | ||
| Acta1(Wt)—tirasemtiv | 8 | 9 | ||||||||||
| Absolute force (mN) | 49 ± 16 | 48 ± 15 | 49 ± 15 | 54 ± 18 | 98 ± 48 | 133 ± 61 | 165 ± 67 | 187 ± 70 | 213 ± 72 | 229 ± 69 | ||
| Normalized force (mN/mm2) | 68 ± 21 | 66 ± 21 | 67 ± 21 | 74 ± 25 | 134 ± 66 | 182 ± 84 | 226 ± 92 | 260 ± 96 | 297 ± 99 | 314 ± 95 | ||
| Relative force (% of maximum) | 22 ± 2 | 22 ± 2 | 21 ± 27 | 23 ± 2 | 43 ± 10a | 61 ± 11a | 77 ± 8a | 86 ± 6 | 97 ± 2 | 100 ± 0.1 | ||
| Acta1(D286G)—vehicle | 14 | 9 | ||||||||||
| Absolute force (mN) | 37 ± 11 | 36 ± 11 | 35 ± 11 | 37 ± 12 | 64 ± 26 | 89 ± 35 | 117 ± 42 | 139 ± 45 | 168 ± 48 | 183 ± 49 | ||
| Normalized force (mN/mm2) | 51 ± 16 | 49 ± 15 | 49 ± 15 | 51 ± 16 | 87 ± 36 | 123 ± 48 | 160 ± 58 | 190 ± 62 | 231 ± 66 | 251 ± 68 | ||
| Relative force (% of maximum) | 21 ± 3 | 20 ± 3 | 20 ± 3 | 22 ± 4 | 34 ± 8 | 49 ± 10 | 63 ± 9 | 75 ± 8 | 92 ± 4 | 100 ± 0.0 | ||
| Acta1(D286G)—tirasemtivb | 12 | 9 | ||||||||||
| Absolute force (mN) | 36 ± 12 | 35 ± 13 | 38 ± 15 | 45 ± 21 | 68 ± 31 | 84 ± 36 | 101 ± 39 | 116 ± 41 | 139 ± 43 | 150 ± 43 | ||
| Normalized force (mN/mm2) | 49 ± 17 | 48 ± 17 | 52 ± 21 | 62 ± 29 | 93 ± 42 | 115 ± 49 | 139 ± 53 | 159 ± 56 | 190 ± 58 | 206 ± 59 | ||
| Relative force (% of maximum) | 23 ± 2 | 23 ± 3 | 25 ± 5 | 30 ± 12a | 44 ± 13a | 54 ± 13 | 66 ± 12 | 76 ± 10 | 92 ± 4 | 100 ± 0.0 | ||
| Frequency (Hz) . | 1 . | 5 . | 10 . | 20 . | 40 . | 60 . | 80 . | 100 . | 150 . | 200 . | # of mice . | Age of mice in months . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Acta1(Wt)—vehicle | 7 | 9 | ||||||||||
| Absolute force (mN) | 52 ± 22 | 50 ± 21 | 49 ± 20 | 49 ± 21 | 92 ± 48 | 140 ± 73 | 178 ± 89 | 204 ± 95 | 237 ± 98 | 248 ± 95 | ||
| Normalized force (mN/mm2) | 72 ± 30 | 68 ± 28 | 67 ± 28 | 67 ± 28 | 127 ± 57 | 192 ± 100 | 244 ± 122 | 280 ± 130 | 325 ± 134 | 340 ± 130 | ||
| Relative force (% of maximum) | 21 ± 2 | 20 ± 2 | 19 ± 2 | 19 ± 2 | 34 ± 7 | 53 ± 9 | 69 ± 8 | 81 ± 6 | 95 ± 3 | 100 ± 0.0 | ||
| Acta1(Wt)—tirasemtiv | 8 | 9 | ||||||||||
| Absolute force (mN) | 49 ± 16 | 48 ± 15 | 49 ± 15 | 54 ± 18 | 98 ± 48 | 133 ± 61 | 165 ± 67 | 187 ± 70 | 213 ± 72 | 229 ± 69 | ||
| Normalized force (mN/mm2) | 68 ± 21 | 66 ± 21 | 67 ± 21 | 74 ± 25 | 134 ± 66 | 182 ± 84 | 226 ± 92 | 260 ± 96 | 297 ± 99 | 314 ± 95 | ||
| Relative force (% of maximum) | 22 ± 2 | 22 ± 2 | 21 ± 27 | 23 ± 2 | 43 ± 10a | 61 ± 11a | 77 ± 8a | 86 ± 6 | 97 ± 2 | 100 ± 0.1 | ||
| Acta1(D286G)—vehicle | 14 | 9 | ||||||||||
| Absolute force (mN) | 37 ± 11 | 36 ± 11 | 35 ± 11 | 37 ± 12 | 64 ± 26 | 89 ± 35 | 117 ± 42 | 139 ± 45 | 168 ± 48 | 183 ± 49 | ||
| Normalized force (mN/mm2) | 51 ± 16 | 49 ± 15 | 49 ± 15 | 51 ± 16 | 87 ± 36 | 123 ± 48 | 160 ± 58 | 190 ± 62 | 231 ± 66 | 251 ± 68 | ||
| Relative force (% of maximum) | 21 ± 3 | 20 ± 3 | 20 ± 3 | 22 ± 4 | 34 ± 8 | 49 ± 10 | 63 ± 9 | 75 ± 8 | 92 ± 4 | 100 ± 0.0 | ||
| Acta1(D286G)—tirasemtivb | 12 | 9 | ||||||||||
| Absolute force (mN) | 36 ± 12 | 35 ± 13 | 38 ± 15 | 45 ± 21 | 68 ± 31 | 84 ± 36 | 101 ± 39 | 116 ± 41 | 139 ± 43 | 150 ± 43 | ||
| Normalized force (mN/mm2) | 49 ± 17 | 48 ± 17 | 52 ± 21 | 62 ± 29 | 93 ± 42 | 115 ± 49 | 139 ± 53 | 159 ± 56 | 190 ± 58 | 206 ± 59 | ||
| Relative force (% of maximum) | 23 ± 2 | 23 ± 3 | 25 ± 5 | 30 ± 12a | 44 ± 13a | 54 ± 13 | 66 ± 12 | 76 ± 10 | 92 ± 4 | 100 ± 0.0 | ||