Table 1. In vitro EDL muscle... | Rockefeller University Press

Table 1.

In vitro EDL muscle mechanics—acute tirasemtiv treatment

Frequency (Hz)	1		5	10	20	40	60	80	100	150	200	# of mice	Age of mice in months
Acta1(Wt)—vehicle												9	8
Absolute force (mN)	34 ± 12		33 ± 11	33 ± 11	35 ± 11	64 ± 26	100 ± 40	136 ± 50	164 ± 55	203 ± 63	221 ± 65
Normalized force (mN/mm²)	35 ± 13		35 ± 13	34,5 ± 13	36 ± 13	66 ± 28	104 ± 43	141 ± 62	170 ± 62	214 ± 71	232 ± 7
Relative force (% of maximum)	16 ± 1		15 ± 1	15 ± 1	16 ± 2	28 ± 6	44 ± 7	61 ± 6	75 ± 5	91 ± 3	100 ± 0.05
**Acta1(Wt)—tirasemtiv**												9	8
Absolute force (mN)	31 ± 13		37 ± 7	44 ± 10	58 ± 16	103 ± 34^{^a}	156 ± 50^{^a}	193 ± 61^{^a}	216 ± 67^{^a}	242 ± 74^{^a}	232 ± 80
Normalized force (mN/mm²)	32 ± 15		39 ± 10	45 ± 13	60 ± 19^{^a}	106 ± 37^{^a}	161 ± 57^{^a}	199 ± 69	222 ± 77	250 ± 85	237 ± 87
Relative force (% of maximum)	13 ± 2		16 ± 2	18 ± 2	24 ± 3^{^a}	42 ± 6^{^a}	63 ± 7^{^a}	78 ± 4^{^a}	87 ± 2^{^a}	98 ± 2^{^a}	100 ± 0.2
Acta1(D286G)—vehicle												9	8
Absolute force (mN)		24 ± 9^{^b}	24 ± 9^{^b}	24 ± 9^{^b}	25 ± 10^{^b}	40 ± 19^{^b}	66 ± 33^{^b}	97 ± 47	121 ± 54	152 ± 60	168 ± 60
Normalized force (mN/mm²)		32 ± 12	31 ± 12	31 ± 12	33 ± 13	52 ± 26	87 ± 45	129 ± 63	159 ± 71	200 ± 79	221 ± 79
Relative force (% of maximum)		14 ± 2	14 ± 2	14 ± 2	15 ± 2	22 ± 6^{^b}	37 ± 9^{^b}	55 ± 10^{^b}	69 ± 5^{^b}	89 ± 3	100 ± 0.0
**Acta1(D286G)—tirasemtiv**												9	8
Absolute force (mN)	26 ± 13		28 ± 10	32 ± 12	43 ± 15	76 ± 31^{^a}	119 ± 48^{^a}	151 ± 55^{^a}	171 ± 58^{^a}	194 ± 61^{^a}	197 ± 62
Normalized force (mN/mm²)	38 ± 11		39.2 ± 12	44.6 ± 13	58.6 ± 18	104.7 ± 39^{^a}	163.9 ± 59^{^a}	206.5 ± 67^{^a}	231.7 ± 71^{^a}	261.3 ± 74^{^a}	264.3 ± 76
Relative force (% of maximum)	13 ± 2		14 ± 3	16 ± 3	21 ± 7^{^a}	37 ± 9^{^a}	58 ± 8^{^a}	74 ± 6^{^a}	85 ± 3^{^a}	97 ± 1^{^a}	100 ± 1.3

Frequency (Hz)	1		5	10	20	40	60	80	100	150	200	# of mice	Age of mice in months
Acta1(Wt)—vehicle												9	8
Absolute force (mN)	34 ± 12		33 ± 11	33 ± 11	35 ± 11	64 ± 26	100 ± 40	136 ± 50	164 ± 55	203 ± 63	221 ± 65
Normalized force (mN/mm²)	35 ± 13		35 ± 13	34,5 ± 13	36 ± 13	66 ± 28	104 ± 43	141 ± 62	170 ± 62	214 ± 71	232 ± 7
Relative force (% of maximum)	16 ± 1		15 ± 1	15 ± 1	16 ± 2	28 ± 6	44 ± 7	61 ± 6	75 ± 5	91 ± 3	100 ± 0.05
**Acta1(Wt)—tirasemtiv**												9	8
Absolute force (mN)	31 ± 13		37 ± 7	44 ± 10	58 ± 16	103 ± 34^{^a}	156 ± 50^{^a}	193 ± 61^{^a}	216 ± 67^{^a}	242 ± 74^{^a}	232 ± 80
Normalized force (mN/mm²)	32 ± 15		39 ± 10	45 ± 13	60 ± 19^{^a}	106 ± 37^{^a}	161 ± 57^{^a}	199 ± 69	222 ± 77	250 ± 85	237 ± 87
Relative force (% of maximum)	13 ± 2		16 ± 2	18 ± 2	24 ± 3^{^a}	42 ± 6^{^a}	63 ± 7^{^a}	78 ± 4^{^a}	87 ± 2^{^a}	98 ± 2^{^a}	100 ± 0.2
Acta1(D286G)—vehicle												9	8
Absolute force (mN)		24 ± 9^{^b}	24 ± 9^{^b}	24 ± 9^{^b}	25 ± 10^{^b}	40 ± 19^{^b}	66 ± 33^{^b}	97 ± 47	121 ± 54	152 ± 60	168 ± 60
Normalized force (mN/mm²)		32 ± 12	31 ± 12	31 ± 12	33 ± 13	52 ± 26	87 ± 45	129 ± 63	159 ± 71	200 ± 79	221 ± 79
Relative force (% of maximum)		14 ± 2	14 ± 2	14 ± 2	15 ± 2	22 ± 6^{^b}	37 ± 9^{^b}	55 ± 10^{^b}	69 ± 5^{^b}	89 ± 3	100 ± 0.0
**Acta1(D286G)—tirasemtiv**												9	8
Absolute force (mN)	26 ± 13		28 ± 10	32 ± 12	43 ± 15	76 ± 31^{^a}	119 ± 48^{^a}	151 ± 55^{^a}	171 ± 58^{^a}	194 ± 61^{^a}	197 ± 62
Normalized force (mN/mm²)	38 ± 11		39.2 ± 12	44.6 ± 13	58.6 ± 18	104.7 ± 39^{^a}	163.9 ± 59^{^a}	206.5 ± 67^{^a}	231.7 ± 71^{^a}	261.3 ± 74^{^a}	264.3 ± 76
Relative force (% of maximum)	13 ± 2		14 ± 3	16 ± 3	21 ± 7^{^a}	37 ± 9^{^a}	58 ± 8^{^a}	74 ± 6^{^a}	85 ± 3^{^a}	97 ± 1^{^a}	100 ± 1.3

Tirasemtiv versus vehicle (P < 0.05).

Acta1(D286G) versus Acta1(wt) (P < 0.05).