Quantitative analyses of mitochondria and CRUs in soleus muscles
| . | A . | B . | C . | D . | E . | F . |
|---|---|---|---|---|---|---|
| . | No. of mitochondria /100 μm2 . | No. of altered mitochondria /100 μm2 (%) . | No. of CRUs /100 μm2 . | % of oblique/longitudinal CRUs . | % of dyads . | No. of mitochondrion/CRU pairs/100 μm2 . |
| WT | 42.5 ± 1.1 | 2.1 ± 0.3 (1%) | 34.8 ± 1.1 | 4.2 ± 0.1 | 6.5 ± 0.1 | 21.6 ± 1.2 |
| Ho | 30.9 ± 1.1* (P = 8.46E-13) | 3.0 ± 0.4* (10%) (P = 2.70E-07) | 28.0 ± 1.0* (P = 1.19E-05) | 14.9 ± 0.1* (P = 8.12E-13) | 14.1 ± 0.1* (P = 3.0E-05) | 12.9 ± 0.7* (P = 2.08E-09) |
| . | A . | B . | C . | D . | E . | F . |
|---|---|---|---|---|---|---|
| . | No. of mitochondria /100 μm2 . | No. of altered mitochondria /100 μm2 (%) . | No. of CRUs /100 μm2 . | % of oblique/longitudinal CRUs . | % of dyads . | No. of mitochondrion/CRU pairs/100 μm2 . |
| WT | 42.5 ± 1.1 | 2.1 ± 0.3 (1%) | 34.8 ± 1.1 | 4.2 ± 0.1 | 6.5 ± 0.1 | 21.6 ± 1.2 |
| Ho | 30.9 ± 1.1* (P = 8.46E-13) | 3.0 ± 0.4* (10%) (P = 2.70E-07) | 28.0 ± 1.0* (P = 1.19E-05) | 14.9 ± 0.1* (P = 8.12E-13) | 14.1 ± 0.1* (P = 3.0E-05) | 12.9 ± 0.7* (P = 2.08E-09) |
In soleus fibers from Ho mice, the frequency of mitochondria, the number of CRUs, and the number of couples mitochondrion/CRU are significantly reduced compared with WT. On the other hand, the number of damaged mitochondria, dyads (i.e., incomplete CRUs), and misoriented CRUs (oblique/longitudinal) are significantly increased compared with WT littermates. Data are shown as mean ± SEM. (* P < 0.001). Samples size: 28 fibers from three WT mice, 28 fibers from three Ho littermates, five micrographs/fiber.