Quantitative analyses of mitochondria and CRUs in EDL muscles
| A | B | C | D | E | F | |
|---|---|---|---|---|---|---|
| EDL | No. of mitochondria/100 μm2 | No. of altered mitochondria/100 μm2 (%) | No. of CRUs/100 μm2 | % of oblique/longitudinal CRUs | % of dyads | No. of mitochondrion/CRU pairs/100 μm2 |
| WT | 22.8 ± 0.8 | 0.4 ± 0.1 (2%) | 46.1 ± 0.9 | 4.3 ± 0.1 | 1.0 ± 0.9 | 18.6 ± 0.7 |
| Ho | 16.8 ± 0.8* (P = 2.25E-06) | 2.0 ± 0.3* (12%) (P = 4.48E-06) | 36.5 ± 0.9* (P = 2.42E-13) | 10.5 ± 0.1* (P = 5.11E-06) | 3.9 ± 0.1* (P = 0.0003) | 12.3 ± 0.7* (P = 1.09E-09) |
| A | B | C | D | E | F | |
|---|---|---|---|---|---|---|
| EDL | No. of mitochondria/100 μm2 | No. of altered mitochondria/100 μm2 (%) | No. of CRUs/100 μm2 | % of oblique/longitudinal CRUs | % of dyads | No. of mitochondrion/CRU pairs/100 μm2 |
| WT | 22.8 ± 0.8 | 0.4 ± 0.1 (2%) | 46.1 ± 0.9 | 4.3 ± 0.1 | 1.0 ± 0.9 | 18.6 ± 0.7 |
| Ho | 16.8 ± 0.8* | 2.0 ± 0.3* (12%) | 36.5 ± 0.9* | 10.5 ± 0.1* | 3.9 ± 0.1* | 12.3 ± 0.7* |
In EDL fibers from Ho mice, the frequency of mitochondria, the number of CRUs, and the number of couples mitochondrion/CRU are significantly reduced compared with WT. On the other hand, the number of damaged mitochondria, dyads (i.e., incomplete CRUs), and misoriented CRUs (oblique/longitudinal) are significantly increased compared with WT littermates. Data are shown as mean ± SEM (* P < 0.001). Samples size: 32 fibers from three WT mice, 22 fibers from three Ho littermates, five micrographs/fiber.