Equilibrium binding parameters derived from isothermal titration calorimetry (pH 8.5)
| . | Gdm+ . | Guanylurea . |
|---|---|---|
| Kd (μM) | 410 ± 40 | 140 ± 30 |
| ΔG (kcal/mol) | −4.6 ± 0.1 | −5.4 ± 0.06 |
| ΔH (kcal/mol) | −4.9 ± 1.0 | −7.2 ± 0.6 |
| TΔS (kcal/mol) | −0.3 ± 0.9 | −1.8 ± 0.6 |
| n | 0.56 ± 0.04 | 0.43 ± 0.03 |
| . | Gdm+ . | Guanylurea . |
|---|---|---|
| Kd (μM) | 410 ± 40 | 140 ± 30 |
| ΔG (kcal/mol) | −4.6 ± 0.1 | −5.4 ± 0.06 |
| ΔH (kcal/mol) | −4.9 ± 1.0 | −7.2 ± 0.6 |
| TΔS (kcal/mol) | −0.3 ± 0.9 | −1.8 ± 0.6 |
| n | 0.56 ± 0.04 | 0.43 ± 0.03 |
Mean and SEM from three independent experiments.