Kd values for substrate binding to Gdx-Clo as a function of pH
| pH . | Gdm+ (mM) ± SEM . | Guanylurea (mM) ± SEM . |
|---|---|---|
| 6.5 | 4.3 ± 1.0 | 0.86 ± 0.22 |
| 7.0 | 1.5 ± 0.3 | 0.19 ± 0.02 |
| 7.5 | 0.96 ± 0.12 | 0.12 ± 0.02 |
| 8.0 | 0.66 ± 0.08 | 0.059 ± 0.001 |
| 8.5 | 0.48 ± 0.10 | 0.091 ± 0.016 |
| 9.0 | Not determined | 0.063 ± 0.005 |
| pH . | Gdm+ (mM) ± SEM . | Guanylurea (mM) ± SEM . |
|---|---|---|
| 6.5 | 4.3 ± 1.0 | 0.86 ± 0.22 |
| 7.0 | 1.5 ± 0.3 | 0.19 ± 0.02 |
| 7.5 | 0.96 ± 0.12 | 0.12 ± 0.02 |
| 8.0 | 0.66 ± 0.08 | 0.059 ± 0.001 |
| 8.5 | 0.48 ± 0.10 | 0.091 ± 0.016 |
| 9.0 | Not determined | 0.063 ± 0.005 |
Values represent mean and SEM of three–four independent titrations from two independent protein preps.