Table 1.

Summary of FDA-approved CAR-T cell product manufacturing

Product nameCommercial nameCell population prior to T-cell activationStarting leukopak storageTransgene integration methodFinal product storageReferences
Tisa-cel Kymriah Enriched T cells Frozen Lentivirus Frozen Fowler et al. (2022), Maude et al. (2018), Schuster et al. (2019), Tyagarajan et al. (2019)  
Axi-cel Yescarta PBMCs (from Ficoll gradient enrichment) Fresh Retrovirus Frozen Jacobson et al. (2022), Locke et al. (2022), Roberts et al. (2018)  
Brexu-cel Tecartus CD19-depleted and CD4/CD8-enriched T cells Fresh Retrovirus Frozen Mian and Hill (2021), Wang et al. (2020)  
Liso-cel Breyanzi CD4 and CD8 T cells separately Not reported Lentivirus Frozen Kamdar et al. (2022), Sehgal et al. (2022), Teoh and Brown (2022)  
Idecabtagene vicleucel Abecma PBMCs Not reported Lentivirus Frozen Al Hadidi et al. (2023), Hansen et al. (2023), Raje et al. (2019)  
Ciltacabtagene autoleucel Carvykti Enriched T cells Frozen Lentivirus Frozen Berdeja et al. (2021); Committee for Medicinal Products for Human Use (2022); San-Miguel et al. (2023)  
Product nameCommercial nameCell population prior to T-cell activationStarting leukopak storageTransgene integration methodFinal product storageReferences
Tisa-cel Kymriah Enriched T cells Frozen Lentivirus Frozen Fowler et al. (2022), Maude et al. (2018), Schuster et al. (2019), Tyagarajan et al. (2019)  
Axi-cel Yescarta PBMCs (from Ficoll gradient enrichment) Fresh Retrovirus Frozen Jacobson et al. (2022), Locke et al. (2022), Roberts et al. (2018)  
Brexu-cel Tecartus CD19-depleted and CD4/CD8-enriched T cells Fresh Retrovirus Frozen Mian and Hill (2021), Wang et al. (2020)  
Liso-cel Breyanzi CD4 and CD8 T cells separately Not reported Lentivirus Frozen Kamdar et al. (2022), Sehgal et al. (2022), Teoh and Brown (2022)  
Idecabtagene vicleucel Abecma PBMCs Not reported Lentivirus Frozen Al Hadidi et al. (2023), Hansen et al. (2023), Raje et al. (2019)  
Ciltacabtagene autoleucel Carvykti Enriched T cells Frozen Lentivirus Frozen Berdeja et al. (2021); Committee for Medicinal Products for Human Use (2022); San-Miguel et al. (2023)  

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