Table 1.

Primers used for real-time PCR

GeneForward (5′ ➨ 3′)Reverse (5′ ➨ 3′)Reference
Mx1 TGT​GGA​CAT​TGC​TAC​CAC​AGA AAG​GCA​GTT​TGG​ACC​ATC​TC Uccellini and García-Sastre (2018)  
Mx2 TGG​CAC​TTC​CAG​TTC​CTC​TCA GGT​TGT​GAG​CCT​CTT​GGC​GG This study 
Pan-Ifna CCT​GAG​GAA/GAG​AAG​AAA​CAC​AGC​C GGC​TCT​CCA​GAC/TTT​CTG​CTC​TG Tailor et al. (2007)  
Ifnb CAG​CTC​CAA​GAA​AGG​ACG​AAC GGC​AGT​GTA​ACT​CTT​CTG​CAT Primerbank ID: 6754304a1 
Ifng CCT​TCT​TCA​GCA​ACA​GCA​AGG​C GAC​TCC​TTT​TCC​GCT​TCC​TGA​G Winer et al. (2001)  
Oas1 ATG​GAG​CAC​GGA​CTC​AGG​A TCA​CAC​ACG​ACA​TTG​ACG​GC Uccellini and García-Sastre (2018)  
Irf3 CGG​AAA​GAA​GTG​TTG​CGG​TTA​GC CAG​GCT​GCT​TTT​GCC​ATT​GGT​G Pang et al. (2018)  
Irf7 GCC​AGG​AGC​AAG​ACC​GTG​TT TGC​CCC​ACC​ACT​GCC​TGT​A Fensterl et al. (2008)  
Irf9 TAA​CCG​CTT​GCC​CTG​CAA​CT ACT​TTG​CCT​GAG​GCC​AAT​CCT​GA This study 
Isg15 CTG​ACT​GTG​AGA​GCA​AGC​AGC ACC​TTT​AGG​TCC​CAG​GCC​ATT​G This study 
Ifit1 CAG​AAG​CAC​ACA​TTG​AAG​AA TGT​AAG​TAG​CCA​GAG​GAA​GG Fensterl et al. (2008)  
Stat1 TCA​CAG​TGG​TTC​GAG​CTT​CAG GCA​AAC​GAG​ACA​TCA​TAG​GCA Uccellini and García-Sastre (2018)  
Il1b CAG​GCA​GGC​AGT​ATC​ACT​CAT​TG CCA​GCA​GGT​TAT​CAT​CAT​CAT​CCC This study 
Il10 GGT​TGC​CAA​GCC​TTA​TCG​GA ACC​TGC​TCC​ACT​GCC​TTG​C Wagage et al. (2014)  
Il6 AAA​GAC​AAA​GCC​AGA​GTC​CTT​CAG TGG​TCC​TTA​GCC​ACT​CCT​TCT​GTG This study 
Il12p35 TAC​TAG​AGA​GAC​TTC​TTC​CAC​AAC​AA TCT​GGT​ACA​TCT​TCA​AGT​CCT​CAT​AG This study 
Il12p40 CTC​AGA​AGC​TAA​CCA​TCT​CCT​GG CAC​AGG​TGA​GGT​TCA​CTG​TTT​C Liu et al. (2013)  
Il27p28 GGC​CAT​GAG​GCT​GGA​TCT​C AAC​ATT​TGA​ATC​CTG​CAG​CCA Maroof and Kaye (2008)  
Il17a CTC​CAG​AAG​GCC​CTC​AGA​CTA GGGTCTTCATTGCGGTGG Hod-Dvorai et al. (2011)  
Tnfa ACG​GCA​TGG​ATC​TCA​AAG​AC GTG​GGT​GAG​GAG​CAC​GTA​GT Okazaki et al. (2016)  
Actb GCT​GAG​AGG​GAA​ATC​GTG​CGT​G CCA​GGG​AGG​AAG​AGG​ATG​CGG Bronner et al. (2018)  
GeneForward (5′ ➨ 3′)Reverse (5′ ➨ 3′)Reference
Mx1 TGT​GGA​CAT​TGC​TAC​CAC​AGA AAG​GCA​GTT​TGG​ACC​ATC​TC Uccellini and García-Sastre (2018)  
Mx2 TGG​CAC​TTC​CAG​TTC​CTC​TCA GGT​TGT​GAG​CCT​CTT​GGC​GG This study 
Pan-Ifna CCT​GAG​GAA/GAG​AAG​AAA​CAC​AGC​C GGC​TCT​CCA​GAC/TTT​CTG​CTC​TG Tailor et al. (2007)  
Ifnb CAG​CTC​CAA​GAA​AGG​ACG​AAC GGC​AGT​GTA​ACT​CTT​CTG​CAT Primerbank ID: 6754304a1 
Ifng CCT​TCT​TCA​GCA​ACA​GCA​AGG​C GAC​TCC​TTT​TCC​GCT​TCC​TGA​G Winer et al. (2001)  
Oas1 ATG​GAG​CAC​GGA​CTC​AGG​A TCA​CAC​ACG​ACA​TTG​ACG​GC Uccellini and García-Sastre (2018)  
Irf3 CGG​AAA​GAA​GTG​TTG​CGG​TTA​GC CAG​GCT​GCT​TTT​GCC​ATT​GGT​G Pang et al. (2018)  
Irf7 GCC​AGG​AGC​AAG​ACC​GTG​TT TGC​CCC​ACC​ACT​GCC​TGT​A Fensterl et al. (2008)  
Irf9 TAA​CCG​CTT​GCC​CTG​CAA​CT ACT​TTG​CCT​GAG​GCC​AAT​CCT​GA This study 
Isg15 CTG​ACT​GTG​AGA​GCA​AGC​AGC ACC​TTT​AGG​TCC​CAG​GCC​ATT​G This study 
Ifit1 CAG​AAG​CAC​ACA​TTG​AAG​AA TGT​AAG​TAG​CCA​GAG​GAA​GG Fensterl et al. (2008)  
Stat1 TCA​CAG​TGG​TTC​GAG​CTT​CAG GCA​AAC​GAG​ACA​TCA​TAG​GCA Uccellini and García-Sastre (2018)  
Il1b CAG​GCA​GGC​AGT​ATC​ACT​CAT​TG CCA​GCA​GGT​TAT​CAT​CAT​CAT​CCC This study 
Il10 GGT​TGC​CAA​GCC​TTA​TCG​GA ACC​TGC​TCC​ACT​GCC​TTG​C Wagage et al. (2014)  
Il6 AAA​GAC​AAA​GCC​AGA​GTC​CTT​CAG TGG​TCC​TTA​GCC​ACT​CCT​TCT​GTG This study 
Il12p35 TAC​TAG​AGA​GAC​TTC​TTC​CAC​AAC​AA TCT​GGT​ACA​TCT​TCA​AGT​CCT​CAT​AG This study 
Il12p40 CTC​AGA​AGC​TAA​CCA​TCT​CCT​GG CAC​AGG​TGA​GGT​TCA​CTG​TTT​C Liu et al. (2013)  
Il27p28 GGC​CAT​GAG​GCT​GGA​TCT​C AAC​ATT​TGA​ATC​CTG​CAG​CCA Maroof and Kaye (2008)  
Il17a CTC​CAG​AAG​GCC​CTC​AGA​CTA GGGTCTTCATTGCGGTGG Hod-Dvorai et al. (2011)  
Tnfa ACG​GCA​TGG​ATC​TCA​AAG​AC GTG​GGT​GAG​GAG​CAC​GTA​GT Okazaki et al. (2016)  
Actb GCT​GAG​AGG​GAA​ATC​GTG​CGT​G CCA​GGG​AGG​AAG​AGG​ATG​CGG Bronner et al. (2018)  
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