Statistical data
| Fig. 1 . | Sample number . | Statistical test . |
|---|---|---|
| Fig. 1, A–C | 1 (cultures), 3 (wells) | N/A |
| Fig. 1 E | 4 (cultures), +Glu: (6 coverslips), 17 (FOV), −Glu: (7 coverslips), 22 (FOV), | Mann–Whitney U test |
| Fig. 1 F | >4 (cultures), +Glu: 10 (wells), −Glu_1h: 7 (wells), −Glu_3h: 7 (wells), Dorsomorphin: 3 (wells) | One-way ANOVA |
| Fig. 2 | Sample number | Statistical test |
| Fig. 2 A | >4 (cultures), +Glu:11 (wells), −Glu_1h: 7 (wells), −Glu_3h: 7 (wells), Dorsomorphine: 3 (wells) | One-way ANOVA |
| Fig. 2 B | 4 (cultures), 16 (wells) per condition | Two-tailed, unpaired t test |
| Fig. 2, C and D | 4 (cultures), 7 (wells) per condition | Mann–Whitney U test |
| Fig. 2, F and G | 6 ad lib mice, 6 IF mice | Mann–Whitney U test |
| Fig. 2 I | 5 (culture), 5 (mitochondrial preps/blots) per condition | One sample t test |
| Fig. 3 | Sample number | Statistical test |
| Fig. 3 B | 3 (cultures), Ctrl: 5 (coverslips), 60 (FOV), Sirt3 KD: 4 (coverslips), 46 (FOV) | Mann–Whitney U test |
| Fig. 3 D | 3 (cultures), 29 (coverslips), 78 (FOV) | Mann–Whitney U test |
| Fig. 3 H | 2 (cultures), 14 (coverslips), 27 (FOV) | Mann–Whitney U test |
| Fig. 4 | Sample number | Statistical test |
| Fig. 4, B and C | 6 (cultures), Ctrl: 32 (coverslips/neurons), Sirt3 KD: 10 (coverslips/neurons) | Kruskal–Wallis test |
| Fig. 4 E | 3 (cultures), 9 (coverslips), Ctrl_1Hz: 2,395 (synapses), Sirt3 KD_1Hz: 1,789 (synapses), Ctrl_10Hz: 2,178 (synapses), Sirt3 KD_10Hz: 1,641 (synapses) | Two-sample t test |
| Fig. 4 G | Ctrl:7 (cultures), 10 (coverslips), 13 (neurons); Sirt3 KD_L/P:5 (cultures), 10 (coverslips), 11 (neurons); rescue L/P- 2 (cultures), 5 (coverslips), 6 (neurons), Sirt3 KD, Glu: 3 (cultures), 6 (coverslips), 10 (neurons) | Kruskal–Wallis test |
| Fig. 4 I | Ctrl: 7 (cultures), 15 (coverslips), 19 (neurons), Sirt3 KD: 5 (cultures), 14 (coverslips), 19 (neurons) | Mann–Whitney U test |
| Fig. S1 | Sample number | Statistical test |
| Fig. S1 A | 3 (cultures), +Glu: 13 (wells), 24 (FOV), −Glu: 14 (wells), 27 (FOV) | Mann–Whitney U test |
| Fig. S1 B | 12 ad lib mice, 12 overnight fasted mice | Mann–Whitney U test |
| Fig. S1 D | 11 ad lib mice, 11 overnight fasted mice | Mann–Whitney U test |
| Fig. S2 A | 3 (cultures), Ctrl: 4 (wells), Sirt3 KD: 4 (wells) | Two-tailed, unpaired t test |
| Fig. S2, B and C | 2 (cultures), Ctrl: 11 (coverslips), Sirt3 KD: 8 (coverslips), Ctrl: 31 (neurons), Sirt3 KD: 42 (neurons) | Two-tailed, unpaired t test |
| Fig. S3 A | 6 (cultures), Ctrl: 32 (coverslips/neurons), Sirt3 KD: 10 (coverslips/neurons) | N/A |
| Fig. S3 B | Ctrl: 4 (cultures), 8 (neurons), Sirt3: KD 2 (cultures), 7 (neurons) | N/A |
| Fig. S3D | 3 (cultures), 8 (neurons) | Wilcoxon test |
| Fig. S3, E and F | 5 (culture), Ctrl:19 (coverslips/neurons), Sirt3 KD:26 (coverslips/neurons) | Two-tailed, unpaired t test |
| Fig. S3, G and H | 5 (culture), Ctrl:13 (coverslips/neurons), Sirt3 KD:20 (coverslips/neurons) | Two-tailed, unpaired t test |
| Fig. S3, I and J | 5 (culture), Ctrl:13 (coverslips/neurons), Sirt3 KD:20 (coverslips/neurons) | Two-tailed, unpaired t test |
| Fig. 1 . | Sample number . | Statistical test . |
|---|---|---|
| Fig. 1, A–C | 1 (cultures), 3 (wells) | N/A |
| Fig. 1 E | 4 (cultures), +Glu: (6 coverslips), 17 (FOV), −Glu: (7 coverslips), 22 (FOV), | Mann–Whitney U test |
| Fig. 1 F | >4 (cultures), +Glu: 10 (wells), −Glu_1h: 7 (wells), −Glu_3h: 7 (wells), Dorsomorphin: 3 (wells) | One-way ANOVA |
| Fig. 2 | Sample number | Statistical test |
| Fig. 2 A | >4 (cultures), +Glu:11 (wells), −Glu_1h: 7 (wells), −Glu_3h: 7 (wells), Dorsomorphine: 3 (wells) | One-way ANOVA |
| Fig. 2 B | 4 (cultures), 16 (wells) per condition | Two-tailed, unpaired t test |
| Fig. 2, C and D | 4 (cultures), 7 (wells) per condition | Mann–Whitney U test |
| Fig. 2, F and G | 6 ad lib mice, 6 IF mice | Mann–Whitney U test |
| Fig. 2 I | 5 (culture), 5 (mitochondrial preps/blots) per condition | One sample t test |
| Fig. 3 | Sample number | Statistical test |
| Fig. 3 B | 3 (cultures), Ctrl: 5 (coverslips), 60 (FOV), Sirt3 KD: 4 (coverslips), 46 (FOV) | Mann–Whitney U test |
| Fig. 3 D | 3 (cultures), 29 (coverslips), 78 (FOV) | Mann–Whitney U test |
| Fig. 3 H | 2 (cultures), 14 (coverslips), 27 (FOV) | Mann–Whitney U test |
| Fig. 4 | Sample number | Statistical test |
| Fig. 4, B and C | 6 (cultures), Ctrl: 32 (coverslips/neurons), Sirt3 KD: 10 (coverslips/neurons) | Kruskal–Wallis test |
| Fig. 4 E | 3 (cultures), 9 (coverslips), Ctrl_1Hz: 2,395 (synapses), Sirt3 KD_1Hz: 1,789 (synapses), Ctrl_10Hz: 2,178 (synapses), Sirt3 KD_10Hz: 1,641 (synapses) | Two-sample t test |
| Fig. 4 G | Ctrl:7 (cultures), 10 (coverslips), 13 (neurons); Sirt3 KD_L/P:5 (cultures), 10 (coverslips), 11 (neurons); rescue L/P- 2 (cultures), 5 (coverslips), 6 (neurons), Sirt3 KD, Glu: 3 (cultures), 6 (coverslips), 10 (neurons) | Kruskal–Wallis test |
| Fig. 4 I | Ctrl: 7 (cultures), 15 (coverslips), 19 (neurons), Sirt3 KD: 5 (cultures), 14 (coverslips), 19 (neurons) | Mann–Whitney U test |
| Fig. S1 | Sample number | Statistical test |
| Fig. S1 A | 3 (cultures), +Glu: 13 (wells), 24 (FOV), −Glu: 14 (wells), 27 (FOV) | Mann–Whitney U test |
| Fig. S1 B | 12 ad lib mice, 12 overnight fasted mice | Mann–Whitney U test |
| Fig. S1 D | 11 ad lib mice, 11 overnight fasted mice | Mann–Whitney U test |
| Fig. S2 A | 3 (cultures), Ctrl: 4 (wells), Sirt3 KD: 4 (wells) | Two-tailed, unpaired t test |
| Fig. S2, B and C | 2 (cultures), Ctrl: 11 (coverslips), Sirt3 KD: 8 (coverslips), Ctrl: 31 (neurons), Sirt3 KD: 42 (neurons) | Two-tailed, unpaired t test |
| Fig. S3 A | 6 (cultures), Ctrl: 32 (coverslips/neurons), Sirt3 KD: 10 (coverslips/neurons) | N/A |
| Fig. S3 B | Ctrl: 4 (cultures), 8 (neurons), Sirt3: KD 2 (cultures), 7 (neurons) | N/A |
| Fig. S3D | 3 (cultures), 8 (neurons) | Wilcoxon test |
| Fig. S3, E and F | 5 (culture), Ctrl:19 (coverslips/neurons), Sirt3 KD:26 (coverslips/neurons) | Two-tailed, unpaired t test |
| Fig. S3, G and H | 5 (culture), Ctrl:13 (coverslips/neurons), Sirt3 KD:20 (coverslips/neurons) | Two-tailed, unpaired t test |
| Fig. S3, I and J | 5 (culture), Ctrl:13 (coverslips/neurons), Sirt3 KD:20 (coverslips/neurons) | Two-tailed, unpaired t test |
FOV: field of view.