Topo IIA SUMOylation site mutants and phenotypes
| Species . | Lysine(s) . | Phenotypes/Functions . | References . |
|---|---|---|---|
| Yeast (S. cerevisiae) | 1220 1246 1247 1277 1278 | SUMOylation of these lysine residues is implicated in: (1) regulating sister centromere cohesion, (2) recruitment of Ipl1 (yeast ortholog of Aurora B) to centromeres in mitosis, and (3) inducing ubiquitination of Top2-DNA cleavage complexes. | (1) Bachant et al. (2002), (2) Edgerton et al. (2016), (3) Sun et al. (2020) |
| 1220 1246 1277 | Required for stable maintenance of minichromosomes. Triple K-to-R mutant stabilizes dicentric chromosomes, indicating reduced kinetochore function. | Takahashi et al. (2006) | |
| Frog (X. Laevis egg extracts) | 660 | Inhibits Topo IIA decatenation activity. Regulates resolution of centromeric catenations in mitosis. | Ryu et al. (2010) |
| 1235 1276 1298 | Recruitment of (1) Claspin, (2) Haspin, and Aurora B to mitotic chromosomes. | (1) Ryu et al. (2015), (2) Yoshida et al. (2016) | |
| Human | 662 | Topo IIA-K662R has reduced Topo IIA centromere localization and increased chromosome segregation defects. | Antoniou-Kourounioti et al. (2019) |
| 1228 1240 | SUMOylation by ZATT/ZNF541 ligase induced by replication fork stalling (hydroxyurea treatment in S-phase) promotes fork reversal and recruitment of PICH DNA translocase. | Tian et al. (2021) | |
| 1240 | Topo IIA-K1240R mutant has reduced Topo IIA centromere localization. | Antoniou-Kourounioti et al. (2019) | |
| 1240 1267 1286 | Triple (K-to-R) mutant reduces Aurora B recruitment to mitotic chromosomes induced by ICRF-193 and partially bypasses the metaphase Topo IIA checkpoint. | Pandey et al. (2020) | |
| 1520 | SUMOylated by NSE2 (Smc5/6 complex) E3 ligase induced by ICRF-193. Topo IIA-K1520R mutant increases chromosome segregation defects but does not affect the G2-phase Topo IIA checkpoint, unlike depletion of Smc5/6 complex components, which does lead to a failure to sustain G2 arrest in response to ICRF-193. | Deiss et al. (2019) |
| Species . | Lysine(s) . | Phenotypes/Functions . | References . |
|---|---|---|---|
| Yeast (S. cerevisiae) | 1220 1246 1247 1277 1278 | SUMOylation of these lysine residues is implicated in: (1) regulating sister centromere cohesion, (2) recruitment of Ipl1 (yeast ortholog of Aurora B) to centromeres in mitosis, and (3) inducing ubiquitination of Top2-DNA cleavage complexes. | (1) Bachant et al. (2002), (2) Edgerton et al. (2016), (3) Sun et al. (2020) |
| 1220 1246 1277 | Required for stable maintenance of minichromosomes. Triple K-to-R mutant stabilizes dicentric chromosomes, indicating reduced kinetochore function. | Takahashi et al. (2006) | |
| Frog (X. Laevis egg extracts) | 660 | Inhibits Topo IIA decatenation activity. Regulates resolution of centromeric catenations in mitosis. | Ryu et al. (2010) |
| 1235 1276 1298 | Recruitment of (1) Claspin, (2) Haspin, and Aurora B to mitotic chromosomes. | (1) Ryu et al. (2015), (2) Yoshida et al. (2016) | |
| Human | 662 | Topo IIA-K662R has reduced Topo IIA centromere localization and increased chromosome segregation defects. | Antoniou-Kourounioti et al. (2019) |
| 1228 1240 | SUMOylation by ZATT/ZNF541 ligase induced by replication fork stalling (hydroxyurea treatment in S-phase) promotes fork reversal and recruitment of PICH DNA translocase. | Tian et al. (2021) | |
| 1240 | Topo IIA-K1240R mutant has reduced Topo IIA centromere localization. | Antoniou-Kourounioti et al. (2019) | |
| 1240 1267 1286 | Triple (K-to-R) mutant reduces Aurora B recruitment to mitotic chromosomes induced by ICRF-193 and partially bypasses the metaphase Topo IIA checkpoint. | Pandey et al. (2020) | |
| 1520 | SUMOylated by NSE2 (Smc5/6 complex) E3 ligase induced by ICRF-193. Topo IIA-K1520R mutant increases chromosome segregation defects but does not affect the G2-phase Topo IIA checkpoint, unlike depletion of Smc5/6 complex components, which does lead to a failure to sustain G2 arrest in response to ICRF-193. | Deiss et al. (2019) |