Table 1. Topo IIA SUMOylation site... | Rockefeller University Press

Table 1.

Topo IIA SUMOylation site mutants and phenotypes

Species	Lysine(s)	Phenotypes/Functions	References
Yeast (S. cerevisiae)	1220 1246 1247 1277 1278	SUMOylation of these lysine residues is implicated in: (1) regulating sister centromere cohesion, (2) recruitment of Ipl1 (yeast ortholog of Aurora B) to centromeres in mitosis, and (3) inducing ubiquitination of Top2-DNA cleavage complexes.	(1) Bachant et al. (2002), (2) Edgerton et al. (2016), (3) Sun et al. (2020)
	1220 1246 1277	Required for stable maintenance of minichromosomes. Triple K-to-R mutant stabilizes dicentric chromosomes, indicating reduced kinetochore function.	Takahashi et al. (2006)
Frog (X. Laevis egg extracts)	660	Inhibits Topo IIA decatenation activity. Regulates resolution of centromeric catenations in mitosis.	Ryu et al. (2010)
	1235 1276 1298	Recruitment of (1) Claspin, (2) Haspin, and Aurora B to mitotic chromosomes.	(1) Ryu et al. (2015), (2) Yoshida et al. (2016)
Human	662	Topo IIA-K662R has reduced Topo IIA centromere localization and increased chromosome segregation defects.	Antoniou-Kourounioti et al. (2019)
	1228 1240	SUMOylation by ZATT/ZNF541 ligase induced by replication fork stalling (hydroxyurea treatment in S-phase) promotes fork reversal and recruitment of PICH DNA translocase.	Tian et al. (2021)
	1240	Topo IIA-K1240R mutant has reduced Topo IIA centromere localization.	Antoniou-Kourounioti et al. (2019)
	1240 1267 1286	Triple (K-to-R) mutant reduces Aurora B recruitment to mitotic chromosomes induced by ICRF-193 and partially bypasses the metaphase Topo IIA checkpoint.	Pandey et al. (2020)
	1520	SUMOylated by NSE2 (Smc5/6 complex) E3 ligase induced by ICRF-193. Topo IIA-K1520R mutant increases chromosome segregation defects but does not affect the G2-phase Topo IIA checkpoint, unlike depletion of Smc5/6 complex components, which does lead to a failure to sustain G2 arrest in response to ICRF-193.	Deiss et al. (2019)

Species	Lysine(s)	Phenotypes/Functions	References
Yeast (S. cerevisiae)	12201246 124712771278	SUMOylation of these lysine residues is implicated in: (1) regulating sister centromere cohesion, (2) recruitment of Ipl1 (yeast ortholog of Aurora B) to centromeres in mitosis, and (3) inducing ubiquitination of Top2-DNA cleavage complexes.	(1) Bachant et al. (2002)(2) Edgerton et al. (2016)(3) Sun et al. (2020)
	122012461277	Required for stable maintenance of minichromosomes. Triple K-to-R mutant stabilizes dicentric chromosomes, indicating reduced kinetochore function.	Takahashi et al. (2006)
Frog (X. Laevis egg extracts)	660	Inhibits Topo IIA decatenation activity. Regulates resolution of centromeric catenations in mitosis.	Ryu et al. (2010)
	12351276 1298	Recruitment of (1) Claspin, (2) Haspin, and Aurora B to mitotic chromosomes.	(1) Ryu et al. (2015)(2) Yoshida et al. (2016)
Human	662	Topo IIA-K662R has reduced Topo IIA centromere localization and increased chromosome segregation defects.	Antoniou-Kourounioti et al. (2019)
	12281240	SUMOylation by ZATT/ZNF541 ligase induced by replication fork stalling (hydroxyurea treatment in S-phase) promotes fork reversal and recruitment of PICH DNA translocase.	Tian et al. (2021)
	1240	Topo IIA-K1240R mutant has reduced Topo IIA centromere localization.	Antoniou-Kourounioti et al. (2019)
	124012671286	Triple (K-to-R) mutant reduces Aurora B recruitment to mitotic chromosomes induced by ICRF-193 and partially bypasses the metaphase Topo IIA checkpoint.	Pandey et al. (2020)
	1520	SUMOylated by NSE2 (Smc5/6 complex) E3 ligase induced by ICRF-193. Topo IIA-K1520R mutant increases chromosome segregation defects but does not affect the G2-phase Topo IIA checkpoint, unlike depletion of Smc5/6 complex components, which does lead to a failure to sustain G2 arrest in response to ICRF-193.	Deiss et al. (2019)