Fly stocks
| Genetic element/strain/transgene . | Notes, stock numbers, Flybase referencea . | Reference . |
|---|---|---|
| Oregon R | Wild-type strain | |
| Histone-2Av-GFP | Ubiquitously expressed chromatin marker | Clarkson and Saint (1999) |
| Rho1 sensor | Rho binding domain from Anillin (aa748–1,239) fused with GFP and expressed from the ubiquitin63E promoter | Munjal et al. (2015) |
| MTD-GAL4 | Maternal triple driver for high levels of GAL4 in the female germline and eggs. B-#31777 | Mazzalupo and Cooley (2006) |
| sqh-moesin-RFP | F-actin binding domain of moesin fused to mRFP and ubiquitously expressed from the Sqh promoter | Kiehart et al. (2000) |
| Patronin-YFP | YFP inserted at the stop codon of the patronin sequence at the endogenous locus | Nashchekin et al. (2016) |
| Khc-mKate | Kinesin-1 heavy chain tagged at the C-terminus with mKate2 in the endogenous locus. B-#67406, FBti0186504 | Gáspár et al. (2017) |
| Khc RNAi | shRNA for Kinesin-1 (TRiP.GL00330 attP2) expressed under control of UAS, B-#35409, FBti0144412 | Perkins et al. (2015), Winkler et al. (2015) |
| UASp-EB1-GFP | Plus-end marker of microtubules expressed from UASp | Jankovics and Brunner (2006) |
| UASp-GFP-slam | Marker for the intercap region in syncytial interphases and basal domains during cellularization | Wenzl et al. (2010) |
| UASp-Utrophin-GFP | F-actin binding domain of human Utrophin tagged with GFP and ubiquitously expressed from the Sqh promoter | Rauzi et al. (2010) |
| sqh-GFP | Myosin regulatory light chain (Sqh) tagged with GFP and expressed as a transgene by its own promoter | Royou et al. (2002) |
| sqh-mKate | Myosin regulatory light chain (Sqh) tagged with mKate and expressed as a transgene by its own promoter | Garcia De Las Bayonas et al. (2019) |
| pebble-GFP | Pebble tagged with GFP at the C-terminus at the endogenous pebble locus | This paper |
| pebble RNAi | shRNAi for pebble (TRiP.GL01092), B-#36841, FBti0146622 | Das et al. (2016) |
| dia-GFP | Dia tagged with GFP at the C-terminus at the endogenous dia locus | Schmidt et al. (2021) |
| zipper-GFP | GFP trap of zipper/Myosin II. (CC01626), B-#51564, FBti0099952 | Buszczak et al. (2007) |
| canoe-YFP | Protein trap of Canoe, CPTI000590, Kyoto stock center, #115111, FBti0144020 | Lowe et al. (2014) |
| par-1-GFP | GFP trap of Par-1 (CC01981), FBti0130199 | Lighthouse et al. (2008), Jiang and Harris (2019) |
| par-1 RNAi | shRNAi for par-1 (TRiP.GL00253), B-#35342, FBti0144338 | Jiang and Harris (2019), Perkins et al. (2015) |
| patronin RNAi | shRNAi for patronin (TRiP.HMS01547), FBti0146670 | Takeda et al. (2018) |
| Resille-GFP | GFP trap in CG8668, FBti0141278 | Takeda et al. (2018) |
| Khc27 | Amorphic allele of Kinesin-1. Recombined with Frt2R for generation of germline clones. B-# 67409, FBal0101625 | Sung et al. (2008) |
| UAS-Khc-GFP | UAS driven expression of Kinesin-1-GFP, B-#9648, FBti0076674 | BDSC cat# 9648 |
| UAS-Kif3c-HA | UAS driven expression of Kif3c, B-#93846, FBti0146622 | Mattie et al. (2010) |
| UAS-mCherry-α-Tubulin | UAS driven expression of mCherry-α-Tubulin, B-#25774, FBti0114317 | Thukral et al. (2020) |
| Genetic element/strain/transgene . | Notes, stock numbers, Flybase referencea . | Reference . |
|---|---|---|
| Oregon R | Wild-type strain | |
| Histone-2Av-GFP | Ubiquitously expressed chromatin marker | Clarkson and Saint (1999) |
| Rho1 sensor | Rho binding domain from Anillin (aa748–1,239) fused with GFP and expressed from the ubiquitin63E promoter | Munjal et al. (2015) |
| MTD-GAL4 | Maternal triple driver for high levels of GAL4 in the female germline and eggs. B-#31777 | Mazzalupo and Cooley (2006) |
| sqh-moesin-RFP | F-actin binding domain of moesin fused to mRFP and ubiquitously expressed from the Sqh promoter | Kiehart et al. (2000) |
| Patronin-YFP | YFP inserted at the stop codon of the patronin sequence at the endogenous locus | Nashchekin et al. (2016) |
| Khc-mKate | Kinesin-1 heavy chain tagged at the C-terminus with mKate2 in the endogenous locus. B-#67406, FBti0186504 | Gáspár et al. (2017) |
| Khc RNAi | shRNA for Kinesin-1 (TRiP.GL00330 attP2) expressed under control of UAS, B-#35409, FBti0144412 | Perkins et al. (2015), Winkler et al. (2015) |
| UASp-EB1-GFP | Plus-end marker of microtubules expressed from UASp | Jankovics and Brunner (2006) |
| UASp-GFP-slam | Marker for the intercap region in syncytial interphases and basal domains during cellularization | Wenzl et al. (2010) |
| UASp-Utrophin-GFP | F-actin binding domain of human Utrophin tagged with GFP and ubiquitously expressed from the Sqh promoter | Rauzi et al. (2010) |
| sqh-GFP | Myosin regulatory light chain (Sqh) tagged with GFP and expressed as a transgene by its own promoter | Royou et al. (2002) |
| sqh-mKate | Myosin regulatory light chain (Sqh) tagged with mKate and expressed as a transgene by its own promoter | Garcia De Las Bayonas et al. (2019) |
| pebble-GFP | Pebble tagged with GFP at the C-terminus at the endogenous pebble locus | This paper |
| pebble RNAi | shRNAi for pebble (TRiP.GL01092), B-#36841, FBti0146622 | Das et al. (2016) |
| dia-GFP | Dia tagged with GFP at the C-terminus at the endogenous dia locus | Schmidt et al. (2021) |
| zipper-GFP | GFP trap of zipper/Myosin II. (CC01626), B-#51564, FBti0099952 | Buszczak et al. (2007) |
| canoe-YFP | Protein trap of Canoe, CPTI000590, Kyoto stock center, #115111, FBti0144020 | Lowe et al. (2014) |
| par-1-GFP | GFP trap of Par-1 (CC01981), FBti0130199 | Lighthouse et al. (2008), Jiang and Harris (2019) |
| par-1 RNAi | shRNAi for par-1 (TRiP.GL00253), B-#35342, FBti0144338 | Jiang and Harris (2019), Perkins et al. (2015) |
| patronin RNAi | shRNAi for patronin (TRiP.HMS01547), FBti0146670 | Takeda et al. (2018) |
| Resille-GFP | GFP trap in CG8668, FBti0141278 | Takeda et al. (2018) |
| Khc27 | Amorphic allele of Kinesin-1. Recombined with Frt2R for generation of germline clones. B-# 67409, FBal0101625 | Sung et al. (2008) |
| UAS-Khc-GFP | UAS driven expression of Kinesin-1-GFP, B-#9648, FBti0076674 | BDSC cat# 9648 |
| UAS-Kif3c-HA | UAS driven expression of Kif3c, B-#93846, FBti0146622 | Mattie et al. (2010) |
| UAS-mCherry-α-Tubulin | UAS driven expression of mCherry-α-Tubulin, B-#25774, FBti0114317 | Thukral et al. (2020) |
Stock numbers according to February 2023.