Table 1. Scoring sheet for humane... | Rockefeller University Press

Table 1.

Scoring sheet for humane endpoints: LPS-induced peritonitis model

Scoring parameter	0	1	2
Conjunctivitis	Normal	Single eye open with visible discharge	Eyes closed with discharge and swelling
Lethargy	Normal locomotion and reaction, >3 steps	Inactive, <3 steps after moderate stimulation, slight hunching	Only lifting of head after moderate stimulation <1 step, severe hunching
Lethargy	Normal locomotion and reaction, >3 steps	Score as 2 for this category	Score as 4 for this category
Hair coat	Well-groomed with smooth coat	Rough coat, minor ruffling	Unkempt fur, dull coat
Grimace pain	Normal	Moderate orbital tightening or nose bulge	Severe orbital tightening, nose bulge, and collapsed ear position
Stool	Normal	Creamy consistency	Watery diarrhea

Scoring parameter	0	1	2
Conjunctivitis	Normal	Single eye open with visible discharge	Eyes closed with discharge and swelling
Lethargy	Normal locomotion and reaction, >3 steps	Inactive, <3 steps after moderate stimulation, slight hunching	Only lifting of head after moderate stimulation <1 step, severe hunching
Lethargy	Normal locomotion and reaction, >3 steps	Score as 2 for this category	Score as 4 for this category
Hair coat	Well-groomed with smooth coat	Rough coat, minor ruffling	Unkempt fur, dull coat
Grimace pain	Normal	Moderate orbital tightening or nose bulge	Severe orbital tightening, nose bulge, and collapsed ear position
Stool	Normal	Creamy consistency	Watery diarrhea

Animals receiving a total score ≥8 or those which have lost 20% of more of their body weight will be euthanized.