Constructs
| Construct . | Source . |
|---|---|
| CFP-ARF6 WT, QL, and TN | Gift from J. Swanson (#11382, 11386, 11387; Addgene) |
| BICD2N-Halo | Full-length mouse BICD2 in the pEGFP vector (gift from A. Akhmanova, Utrecht University, Utrecht, Netherlands) was used to generate a truncated construct spanning residues 1–572 fused to the HaloTag and cloned into pcDNA3.1 |
| Halo-JIP3 (EGFP vector) | HaloTag fused to the N-terminus of hJIP3 from cDNA Clone (GE: MGC9053013) fused in pEGFP vector backbone |
| Halo-JIP4 (CMV vector) | Subcloned from pGEXP1-JIP4 #DU27651, acquired from MRC Protein Phosphorylation and Ubiquitylation Unit reagents and services, University of Dundee, Dundee, UK |
| Halo-JIP3 (CMV vector) | Halo-JIP3 sequence (from EGFP vector construct) subcloned into (Halo-JIP4) CMV vector |
| Halo-JIP4 (EGFP vector) | Halo-JIP4 sequence (from CMV vector construct) subcloned into (Halo-JIP3) EGFP vector |
| K560-Halo | First 560 aa of human KIF5B from pET17: K560 GFP ST (gift from R. Vale, University of California, San Francisco, San Francisco, CA, USA) subcloned into pHTC-HaloTag CMVneo vector (Promega) |
| KIF5C-Halo | Full-length mouse kinesin-1 heavy chain (KIF5C) in pRK5 myc plasmid (gift from J. Kittler, University of Surrey, Guildford, UK) with HaloTag fused to C-terminus |
| GFP-KLC2 | Full-length WT mouse KLC2 in CB6 driven expression vector |
| LAMP1-mNEON | Gift from D. Gadella (plasmid #98882; Addgene) |
| mCh-EGFP-LC3B | Gift from T. Johansen, University of Tromsø, Tromsø, Norway |
| mSc-LC3B | Subcloned from #21073 and #85054; Addgene |
| HA-LIS1 | Gift from D. Smith, University of South Carolina, Columbia, SC, USA |
| EGFP-RAB10 | Gift from M. Scidmore (plasmid #49472; Addgene) |
| Construct . | Source . |
|---|---|
| CFP-ARF6 WT, QL, and TN | Gift from J. Swanson (#11382, 11386, 11387; Addgene) |
| BICD2N-Halo | Full-length mouse BICD2 in the pEGFP vector (gift from A. Akhmanova, Utrecht University, Utrecht, Netherlands) was used to generate a truncated construct spanning residues 1–572 fused to the HaloTag and cloned into pcDNA3.1 |
| Halo-JIP3 (EGFP vector) | HaloTag fused to the N-terminus of hJIP3 from cDNA Clone (GE: MGC9053013) fused in pEGFP vector backbone |
| Halo-JIP4 (CMV vector) | Subcloned from pGEXP1-JIP4 #DU27651, acquired from MRC Protein Phosphorylation and Ubiquitylation Unit reagents and services, University of Dundee, Dundee, UK |
| Halo-JIP3 (CMV vector) | Halo-JIP3 sequence (from EGFP vector construct) subcloned into (Halo-JIP4) CMV vector |
| Halo-JIP4 (EGFP vector) | Halo-JIP4 sequence (from CMV vector construct) subcloned into (Halo-JIP3) EGFP vector |
| K560-Halo | First 560 aa of human KIF5B from pET17: K560 GFP ST (gift from R. Vale, University of California, San Francisco, San Francisco, CA, USA) subcloned into pHTC-HaloTag CMVneo vector (Promega) |
| KIF5C-Halo | Full-length mouse kinesin-1 heavy chain (KIF5C) in pRK5 myc plasmid (gift from J. Kittler, University of Surrey, Guildford, UK) with HaloTag fused to C-terminus |
| GFP-KLC2 | Full-length WT mouse KLC2 in CB6 driven expression vector |
| LAMP1-mNEON | Gift from D. Gadella (plasmid #98882; Addgene) |
| mCh-EGFP-LC3B | Gift from T. Johansen, University of Tromsø, Tromsø, Norway |
| mSc-LC3B | Subcloned from #21073 and #85054; Addgene |
| HA-LIS1 | Gift from D. Smith, University of South Carolina, Columbia, SC, USA |
| EGFP-RAB10 | Gift from M. Scidmore (plasmid #49472; Addgene) |