Key resources
| Reagent or Resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-actin | Cat#MCA-5J11; EnCor | RRID:AB_2572218 |
| Rabbit monoclonal anti-enolase-2 | Cat#D20H2; Cell Signaling Technology | RRID:AB_2099308 |
| Rabbit monoclonal anti-GAPDH | Cat#D16H11; Cell Signaling Technology | RRID:AB_10622025 |
| Rabbit polyclonal anti-GAPDH | Cat#G9545; Sigma-Aldrich | RRID:AB_796208 |
| Human anti-GFP (recombinant antibody) | Institut Curie | hVHH antiGFP-hFc |
| Human anti-NMII (SF9; recombinant antibody) | Institut Curie | SF9-hFc2 A-R-H#01 |
| Rabbit anti-NMII (SF9) | Institut Curie | SF9-RFc A-R-R#42 |
| Rabbit monoclonal anti-PGAM1 | Cat#D3J9T; Cell Signaling Technology | RRID:AB_2736922 |
| Rabbit monoclonal anti-PGK1 (EPR19057) | Cat#AB199438 or EPR19057; Abcam | N/A |
| Rabbit monoclonal anti-PKM1/2 | Cat#C103A3; Cell Signaling Technology | RRID:AB_2163695 |
| Rabbit monoclonal anti-PKM2 | Cat#D78A4; Cell Signaling Technology | RRID:AB_1904096 |
| Rabbit polyclonal anti-phospho-myosin (RLC) | Cat#600-401-416; Rockland | RRID:AB_217940 |
| Mouse monoclonal anti-ß-tubulin | Cat#MCA-1B12; EnCor | RRID:AB_2572389 |
| Goat anti-rabbit HRP-linked antibody | Cat#7074; Cell Signaling Technology | RRID:AB_2099233 |
| Horse anti-mouse HRP-linked antibody | Cat#7076; Cell Signaling Technology | RRID:AB_330924 |
| Chemicals, peptides, and recombinant proteins | ||
| Calpeptin | Cytoskeleton, Inc. Sigma-Aldrich | CNO1 C8999 |
| WIN 55,212-2 (WIN) | Tocris | 1038 |
| Dulbecco’s D phosphate buffered saline 10× | Thermo Fisher Scientific (Gibco) | 14200-067 |
| Neurobasal medium (1×) [−] glutamine | Thermo Fisher Scientific (Gibco) | 21103-049 |
| B-27 supplement (50×) | Thermo Fisher Scientific (Gibco) | 17504-044 |
| Pen Strep (penicillin 10k U/ml streptomycin) 10 mg/ml | Thermo Fisher Scientific (Gibco) | 15140-22 |
| Poly-D-lysine hydrobromide | Sigma-Aldrich | P7280 |
| Laminin | Sigma-Aldrich | L2020 |
| GlutaMAX (100X) | Thermo Fisher Scientific (Gibco) | 35050-038 |
| D(+)-glucose | Sigma-Aldrich | G7021 |
| Sodium pyruvate 100 mM | Thermo Fisher Scientific (Gibco) | 11360070 |
| Oligomycin | Sigma-Aldrich | O4876 |
| 2-Deoxy-D-glucose | Sigma-Aldrich | D8375 |
| Lipofectamine 2000 | Thermo Fisher Scientific (Invitrogen) | 2393037 |
| HBSS 1M | Sigma-Aldrich | H0887 |
| Triton X-100 | Sigma-Aldrich | 93443-500ML |
| Albumine bovin fraction V (BSA) | Euromedex | 04-100-810-E |
| Trypsin inhibitor, soybean | Thermo Fisher Scientific (Gibco) | 17075-029 |
| DNase I | Roche | 04716728001 |
| Trypsin | Sigma-Aldrich | T-6763 |
| Paraformaldehyde | Sigma-Aldrich | P6148 |
| D(+)-saccharose | VWR | 27480.294 |
| Fluoroshield histology mounting medium | Sigma-Aldrich | F6182 |
| ATTO488-phalloidin | Sigma-Aldrich | 49409 |
| Polyethylene glycol (MW 35 kD) | Sigma-Aldrich | 81310 |
| Phalloidin | Sigma-Aldrich | P2141 |
| Taxol | Sigma-Aldrich | PHL89806 |
| DMEM | Sigma-Aldrich | D2650 |
| Trypsin | Promega | V511B |
| Halt protease and phosphatase inhibitor cocktail | Thermo Fisher Scientific | #1861281 |
| Critical commercial assays | ||
| CellTiter-Glo luminescent cell viability assay | Promega | G7570 |
| Seahorse XF analyzer | Proteigene/Agilent | XFp |
| XFp cell culture miniplates and XFp extracellular flux cartridges | Proteigene/Agilent | 103025-100 102022-100 |
| Duolink in situ PLA probe anti-human MINUS | Sigma-Aldrich | DUO92021 |
| Duolink in situ PLA probe anti-mouse MINUS | Sigma-Aldrich | DUO92004 |
| Duolink in situ PLA probe anti-mouse PLUS | Sigma-Aldrich | DUO92004 |
| Duolink in situ PLA probe anti-rabbit PLUS | Sigma-Aldrich | DUO82002 |
| Pierce BCA protein assay kit | Thermo Fisher Scientific | 23225 |
| Experimental models: Cell models | ||
| HEK293 | ATCC CRL-1573 | RRID:CVCL_0045 |
| Primary cortical and hippocampal neuronal cultures from E18 rat embryos | Kaech et al. (2012); this paper | N/A |
| Experimental models: Organisms/strains | ||
| Rat: Sprague Dawley | Janvier Labs RN-SD-F | RRID:RGD_734476 |
| Oligonucleotides | ||
| siRNA targeting sequence: rat GAPDH (1) 5′-CGUAUCGGACGCCUGGUUA-3′ | Sigma-Aldrich | SASI_Rn01_0003788 |
| siRNA targeting sequence: rat GAPDH (2) 5′-CUUCUCUCGAAUACCAUCA-3′ | Sigma-Aldrich | SASI_Rn02_00261166 |
| siRNA Universal Negative Control #1 | Sigma-Aldrich | SIC001-1NMOL |
| Recombinant DNA | ||
| Plasmid: Lifeact-mCherry | Riedl et al. (2008) | https://www.addgene.org/67303/ |
| Plasmid: CB1-CFP | Roland et al. (2014) | N/A |
| Plasmid: GAPDH-eGFP | Dastoor and Dreyer (2001) | N/A |
| Software and algorithms | ||
| NIS- Elements AR | Nikon | Version 4.30.01 (Build 1021) |
| Rstudio | RStudio | R Studio (RRID:SCR_000432) https://www.r-project.org/ |
| Fiji | Schindelin et al. (2012) | Fiji (RRID:SCR_002285) https://imagej.net/Fiji/Downloads |
| jamovi (Version 1.2) | The jamovi project, 2020 | jamovi (RRID:SCR_016142) https://www.jamovi.org |
| Stabilizer plug-in | https://www.cs.cmu.edu/∼kangli/code/Image_Stabilizer.html | ImageJ (RRID:SCR_003070) |
| Kymo Tool Box plug-in | Zala et al. (2013) | https://github.com/fabricecordelieres/IJ-Plugin_KymoToolBox |
| GO enrichment analysis (PANTHER Version 14) | GO (RRID:SCR_002811) https://geneontology.org | |
| Peaks online X software | build 1.6, Bioinformatics Solution, Inc | https://www.bioinfor.com// |
| Rattus norvegicus database | SwissProt release 2022_01, 8,147 entries | https://www.expasy.org |
| Other | ||
| 4–20% Mini-PROTEAN TGX stain-free protein gels | Biorad | 4568095 |
| Trans-Blot Turbo Mini 0.2 µm PVDF transfer packs | Biorad | 1704156 |
| SuperSignal West Pico PLUS chemiluminescent substrate | Thermo Fisher Scientific | 34579 |
| FluoroDish, tissue culture dish with cover glass bottom | World Precision Instruments, Inc. | FD35-100 |
| SYLGARD 184 silicone elastomer kit | Dow Corning | DC184 |
| Reagent or Resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-actin | Cat#MCA-5J11; EnCor | RRID:AB_2572218 |
| Rabbit monoclonal anti-enolase-2 | Cat#D20H2; Cell Signaling Technology | RRID:AB_2099308 |
| Rabbit monoclonal anti-GAPDH | Cat#D16H11; Cell Signaling Technology | RRID:AB_10622025 |
| Rabbit polyclonal anti-GAPDH | Cat#G9545; Sigma-Aldrich | RRID:AB_796208 |
| Human anti-GFP (recombinant antibody) | Institut Curie | hVHH antiGFP-hFc |
| Human anti-NMII (SF9; recombinant antibody) | Institut Curie | SF9-hFc2 A-R-H#01 |
| Rabbit anti-NMII (SF9) | Institut Curie | SF9-RFc A-R-R#42 |
| Rabbit monoclonal anti-PGAM1 | Cat#D3J9T; Cell Signaling Technology | RRID:AB_2736922 |
| Rabbit monoclonal anti-PGK1 (EPR19057) | Cat#AB199438 or EPR19057; Abcam | N/A |
| Rabbit monoclonal anti-PKM1/2 | Cat#C103A3; Cell Signaling Technology | RRID:AB_2163695 |
| Rabbit monoclonal anti-PKM2 | Cat#D78A4; Cell Signaling Technology | RRID:AB_1904096 |
| Rabbit polyclonal anti-phospho-myosin (RLC) | Cat#600-401-416; Rockland | RRID:AB_217940 |
| Mouse monoclonal anti-ß-tubulin | Cat#MCA-1B12; EnCor | RRID:AB_2572389 |
| Goat anti-rabbit HRP-linked antibody | Cat#7074; Cell Signaling Technology | RRID:AB_2099233 |
| Horse anti-mouse HRP-linked antibody | Cat#7076; Cell Signaling Technology | RRID:AB_330924 |
| Chemicals, peptides, and recombinant proteins | ||
| Calpeptin | Cytoskeleton, Inc. Sigma-Aldrich | CNO1 C8999 |
| WIN 55,212-2 (WIN) | Tocris | 1038 |
| Dulbecco’s D phosphate buffered saline 10× | Thermo Fisher Scientific (Gibco) | 14200-067 |
| Neurobasal medium (1×) [−] glutamine | Thermo Fisher Scientific (Gibco) | 21103-049 |
| B-27 supplement (50×) | Thermo Fisher Scientific (Gibco) | 17504-044 |
| Pen Strep (penicillin 10k U/ml streptomycin) 10 mg/ml | Thermo Fisher Scientific (Gibco) | 15140-22 |
| Poly-D-lysine hydrobromide | Sigma-Aldrich | P7280 |
| Laminin | Sigma-Aldrich | L2020 |
| GlutaMAX (100X) | Thermo Fisher Scientific (Gibco) | 35050-038 |
| D(+)-glucose | Sigma-Aldrich | G7021 |
| Sodium pyruvate 100 mM | Thermo Fisher Scientific (Gibco) | 11360070 |
| Oligomycin | Sigma-Aldrich | O4876 |
| 2-Deoxy-D-glucose | Sigma-Aldrich | D8375 |
| Lipofectamine 2000 | Thermo Fisher Scientific (Invitrogen) | 2393037 |
| HBSS 1M | Sigma-Aldrich | H0887 |
| Triton X-100 | Sigma-Aldrich | 93443-500ML |
| Albumine bovin fraction V (BSA) | Euromedex | 04-100-810-E |
| Trypsin inhibitor, soybean | Thermo Fisher Scientific (Gibco) | 17075-029 |
| DNase I | Roche | 04716728001 |
| Trypsin | Sigma-Aldrich | T-6763 |
| Paraformaldehyde | Sigma-Aldrich | P6148 |
| D(+)-saccharose | VWR | 27480.294 |
| Fluoroshield histology mounting medium | Sigma-Aldrich | F6182 |
| ATTO488-phalloidin | Sigma-Aldrich | 49409 |
| Polyethylene glycol (MW 35 kD) | Sigma-Aldrich | 81310 |
| Phalloidin | Sigma-Aldrich | P2141 |
| Taxol | Sigma-Aldrich | PHL89806 |
| DMEM | Sigma-Aldrich | D2650 |
| Trypsin | Promega | V511B |
| Halt protease and phosphatase inhibitor cocktail | Thermo Fisher Scientific | #1861281 |
| Critical commercial assays | ||
| CellTiter-Glo luminescent cell viability assay | Promega | G7570 |
| Seahorse XF analyzer | Proteigene/Agilent | XFp |
| XFp cell culture miniplates and XFp extracellular flux cartridges | Proteigene/Agilent | 103025-100 102022-100 |
| Duolink in situ PLA probe anti-human MINUS | Sigma-Aldrich | DUO92021 |
| Duolink in situ PLA probe anti-mouse MINUS | Sigma-Aldrich | DUO92004 |
| Duolink in situ PLA probe anti-mouse PLUS | Sigma-Aldrich | DUO92004 |
| Duolink in situ PLA probe anti-rabbit PLUS | Sigma-Aldrich | DUO82002 |
| Pierce BCA protein assay kit | Thermo Fisher Scientific | 23225 |
| Experimental models: Cell models | ||
| HEK293 | ATCC CRL-1573 | RRID:CVCL_0045 |
| Primary cortical and hippocampal neuronal cultures from E18 rat embryos | Kaech et al. (2012); this paper | N/A |
| Experimental models: Organisms/strains | ||
| Rat: Sprague Dawley | Janvier Labs RN-SD-F | RRID:RGD_734476 |
| Oligonucleotides | ||
| siRNA targeting sequence: rat GAPDH (1) 5′-CGUAUCGGACGCCUGGUUA-3′ | Sigma-Aldrich | SASI_Rn01_0003788 |
| siRNA targeting sequence: rat GAPDH (2) 5′-CUUCUCUCGAAUACCAUCA-3′ | Sigma-Aldrich | SASI_Rn02_00261166 |
| siRNA Universal Negative Control #1 | Sigma-Aldrich | SIC001-1NMOL |
| Recombinant DNA | ||
| Plasmid: Lifeact-mCherry | Riedl et al. (2008) | https://www.addgene.org/67303/ |
| Plasmid: CB1-CFP | Roland et al. (2014) | N/A |
| Plasmid: GAPDH-eGFP | Dastoor and Dreyer (2001) | N/A |
| Software and algorithms | ||
| NIS- Elements AR | Nikon | Version 4.30.01 (Build 1021) |
| Rstudio | RStudio | R Studio (RRID:SCR_000432) https://www.r-project.org/ |
| Fiji | Schindelin et al. (2012) | Fiji (RRID:SCR_002285) https://imagej.net/Fiji/Downloads |
| jamovi (Version 1.2) | The jamovi project, 2020 | jamovi (RRID:SCR_016142) https://www.jamovi.org |
| Stabilizer plug-in | https://www.cs.cmu.edu/∼kangli/code/Image_Stabilizer.html | ImageJ (RRID:SCR_003070) |
| Kymo Tool Box plug-in | Zala et al. (2013) | https://github.com/fabricecordelieres/IJ-Plugin_KymoToolBox |
| GO enrichment analysis (PANTHER Version 14) | GO (RRID:SCR_002811) https://geneontology.org | |
| Peaks online X software | build 1.6, Bioinformatics Solution, Inc | https://www.bioinfor.com// |
| Rattus norvegicus database | SwissProt release 2022_01, 8,147 entries | https://www.expasy.org |
| Other | ||
| 4–20% Mini-PROTEAN TGX stain-free protein gels | Biorad | 4568095 |
| Trans-Blot Turbo Mini 0.2 µm PVDF transfer packs | Biorad | 1704156 |
| SuperSignal West Pico PLUS chemiluminescent substrate | Thermo Fisher Scientific | 34579 |
| FluoroDish, tissue culture dish with cover glass bottom | World Precision Instruments, Inc. | FD35-100 |
| SYLGARD 184 silicone elastomer kit | Dow Corning | DC184 |