Quantitative summary of yeast actin-binding proteins
| S. cerevisiae name . | Common name . | MW (kD) . | Cellular abundance (ng/µg)a . | nb . | Cellular concentration (µM)c . | Number of molecules per celld . | % free in cytosole . | Cytosolic concentration (µM)f . |
|---|---|---|---|---|---|---|---|---|
| Act1 | Actin | 41.8 | 7.99 ± 1.48 | 11 | 13.2 ± 2.44 | 2.17 × 105 | - | - |
| Tpm1 | Tropomyosin | 23.5 | 4.22 ± 1.08 | 6 | 12.4 ± 3.16 | 2.03 × 105 | 89 ± 8 | 11 ± 0.98 |
| Pfy1g | Profilin | 13.6 | 1.54 ± 0.27 | - | 7.73 ± 1.38 | 1.27 × 105 | - | - |
| Cof1 | Cofilin | 16 | 1.76 ± 0.78 | 3 | 7.58 ± 3.36 | 1.25 × 105 | - | - |
| Abp1 | Actin-binding protein 1 | 65.6 | 4.91 ± 0.79 | 2 | 5.15 ± 0.83 | 8.47 × 104 | 61 ± 11 | 3.2 ± 0.60 |
| Srv2/CAP | Cyclase-associated protein | 57.6 | 2.41 ± 0.36 | 5 | 2.88 ± 0.43 | 4.74 × 104 | 78 ± 9 | 2.2 ± 0.26 |
| Abp140g | Actin binding protein | 71.4 | 2.94 | - | 2.83 ± 0.60 | 4.65 × 104 | 87 ± 8 | 2.5 ± 0.23 |
| Tpm2h | Tropomyosin | 19.1 | 0.70 | - | 2.54 | 4.17 × 104 | 91 ± 8 | 2.3 ± 0.20 |
| Aip1 | Actin-interacting protein 1 | 67.4 | 1.81 ± 0.02 | 2 | 1.85 ± 0.02 | 2.40 × 104 | 82 ± 7 | 1.5 ± 0.13 |
| Cap1/2 | Capping protein (α/β dimer) | 64.3 | 1.43 ± 0.53 | 7 | 1.46 ± 0.54 | 5.12 × 104 | 67 ± 8 | 0.9 ± 0.15 |
| Crn1 | Coronin | 72.6 | 1.48 ± 0.28 | 6 | 1.40 ± 0.27 | 2.31 × 104 | 63 ± 11 | 0.9 ± 0.15 |
| Sac6 | Fimbrin | 71.8 | 1.26 ± 0.02 | 2 | 1.21 ± 0.02 | 1.99 × 104 | 80 ± 8 | 1.0 ± 0.10 |
| Twf1 | Twinfilin | 37.1 | 0.59 ± 0.19 | 8 | 1.10 ± 0.35 | 1.80 × 104 | 71 ± 12 | 0.8 ± 0.13 |
| Arp2 | Actin-related protein 2 | 44.1 | 0.63 ± 0.20 | 4 | 0.98 ± 0.31 | 1.62 × 104 | 81 ± 7 | 0.8 ± 0.07 |
| Scp1 | Calponin/transgelin | 22.8 | 0.28 ± 0.05 | 3 | 0.85 ± 0.15 | 1.39 × 104 | 74 ± 13 | 0.6 ± 0.11 |
| S. cerevisiae name . | Common name . | MW (kD) . | Cellular abundance (ng/µg)a . | nb . | Cellular concentration (µM)c . | Number of molecules per celld . | % free in cytosole . | Cytosolic concentration (µM)f . |
|---|---|---|---|---|---|---|---|---|
| Act1 | Actin | 41.8 | 7.99 ± 1.48 | 11 | 13.2 ± 2.44 | 2.17 × 105 | - | - |
| Tpm1 | Tropomyosin | 23.5 | 4.22 ± 1.08 | 6 | 12.4 ± 3.16 | 2.03 × 105 | 89 ± 8 | 11 ± 0.98 |
| Pfy1g | Profilin | 13.6 | 1.54 ± 0.27 | - | 7.73 ± 1.38 | 1.27 × 105 | - | - |
| Cof1 | Cofilin | 16 | 1.76 ± 0.78 | 3 | 7.58 ± 3.36 | 1.25 × 105 | - | - |
| Abp1 | Actin-binding protein 1 | 65.6 | 4.91 ± 0.79 | 2 | 5.15 ± 0.83 | 8.47 × 104 | 61 ± 11 | 3.2 ± 0.60 |
| Srv2/CAP | Cyclase-associated protein | 57.6 | 2.41 ± 0.36 | 5 | 2.88 ± 0.43 | 4.74 × 104 | 78 ± 9 | 2.2 ± 0.26 |
| Abp140g | Actin binding protein | 71.4 | 2.94 | - | 2.83 ± 0.60 | 4.65 × 104 | 87 ± 8 | 2.5 ± 0.23 |
| Tpm2h | Tropomyosin | 19.1 | 0.70 | - | 2.54 | 4.17 × 104 | 91 ± 8 | 2.3 ± 0.20 |
| Aip1 | Actin-interacting protein 1 | 67.4 | 1.81 ± 0.02 | 2 | 1.85 ± 0.02 | 2.40 × 104 | 82 ± 7 | 1.5 ± 0.13 |
| Cap1/2 | Capping protein (α/β dimer) | 64.3 | 1.43 ± 0.53 | 7 | 1.46 ± 0.54 | 5.12 × 104 | 67 ± 8 | 0.9 ± 0.15 |
| Crn1 | Coronin | 72.6 | 1.48 ± 0.28 | 6 | 1.40 ± 0.27 | 2.31 × 104 | 63 ± 11 | 0.9 ± 0.15 |
| Sac6 | Fimbrin | 71.8 | 1.26 ± 0.02 | 2 | 1.21 ± 0.02 | 1.99 × 104 | 80 ± 8 | 1.0 ± 0.10 |
| Twf1 | Twinfilin | 37.1 | 0.59 ± 0.19 | 8 | 1.10 ± 0.35 | 1.80 × 104 | 71 ± 12 | 0.8 ± 0.13 |
| Arp2 | Actin-related protein 2 | 44.1 | 0.63 ± 0.20 | 4 | 0.98 ± 0.31 | 1.62 × 104 | 81 ± 7 | 0.8 ± 0.07 |
| Scp1 | Calponin/transgelin | 22.8 | 0.28 ± 0.05 | 3 | 0.85 ± 0.15 | 1.39 × 104 | 74 ± 13 | 0.6 ± 0.11 |
Proteins are listed in order from highest to lowest cellular concentration.
Abundance (mean ± SD) is expressed as ng of ABP per µg of total cellular protein determined by quantitative Western blotting as in Fig. S1.
n, number of times cellular abundance was determined by quantitative Western blotting.
Cellular concentrations of proteins were calculated as in Fig. 1 A and Materials and methods.
Number of molecules per cell calculated from the cellular concentration of each protein and the average cytosolic volume of a yeast cell, calculated in Fig. S1.
Percentage of ABP in the cytosol (mean ± SD) was calculated by live imaging as shown in Fig. 2.
Cytosolic concentrations of ABPs; data from Fig. 3 C.
Concentration of these ABPs were determined based on fluorescence of GFP fusions rather than Western blot (details in Materials and methods).
Concentration based on previous study (Drees et al., 1995) showing that Tpm2 is approximately sixfold less abundant than Tpm1 in cells.