Table 2.

List of proteins significantly only found and increased at least fourfold from FAP256A/B-KO compared with CU428 along with their human homologs, known localization, percent coverage, emPAI score, and number of peptides

Only found or increased at least fourfold in FAP256A/B-KO cellsUniprot IDNameAmino acidsHuman homolog?% identityKnown localization?Percent coverage (highest of repeats)emPAI scoreNumber of peptides (average of FAP256A/B-KO)
Only in mutant Q66T67 Chlamydomonas - Heat shock protein 90C - False positive - one peptide from ABC transporter, one peptide from HSP82 180        
Only in mutant I7MLD8 14-3-3 family protein 14-3-3 beta/zeta 254 14-3-3 protein 46.94 Ubiquitous 46.1 1.43 7.3  
Only in mutant I7MIJ3 Phospholipid scramblase 270    5.19 0.099 1  
Only in mutant W7XFI6 Cation channel family transporter 2914 Voltage-dependent L-type calcium channel  43 Membrane 4.7 0.078 8.3  
Only in mutant I7M0U0 Mg2+-specific channel-like exchanger, putative 625 Sodium/potassium/calcium exchanger 3 30 Membrane 8.16 0.209 4.7  
Only in mutant Q9GPP3 Immobilization antigen LB 316    55.1 2.028 8  
Only in mutant Q951C1 Ymf66 446    6.28 0.057 1  
Only in mutant I7LX36 C2 NT-type domain-containing protein 832    1.2 0.03 1  
Only in mutant Q22AC6 PAS domain S-box protein 1177    0.77 0.021 1  
Only in mutant Q22NR4 Arrestin 331    11.2 0.105 1.3  
Only in mutant Q232M6 14-3-3 family epsilon domain protein 246 14-3-3 protein 47.03 Ubiquitous 40.7 0.714 5.3  
Only in mutant Q232G0 Immobilization antigen 316 EPH receptor B1 33.33 Membrane 55.1 1.488 5  
Only in mutant Q232G1 Immobilization antigen 148 EPH receptor B1 28.24 Membrane 50 2.302 2  
Only in mutant Q23JY5 Arrestin 391    5.63 0.114 1.7  
Only in mutant I7LT86 Transmembrane protein, putative 871    9.41 0.155 4.7  
Increased at least fourfold I7M849 ABC transporter family protein 1831 ABC3 31.97 Membrane 17 0.295 17  
Increased at least fourfold I7MJL8 Uncharacterized protein 851    3.06 0.05 1.7  
Increased at least fourfold Q234Y0 Uncharacterized protein 115    31.3 0.471 1.7  
Increased at least fourfold Q22KL2 Sel1 domain protein 766 Protein sel-1 homolog 2 28.74 Endoplasmic reticulum 4.31 0.056 1.7  
Increased at least fourfold I7MMI9 Multidrug resistance protein-like transporter family ABC domain protein 1289 ATP-dependent translocase ABCB1 36.38 Membrane 12.6 0.221 9.3  
Increased at least fourfold Q23Q30 Regulator of Vps4 activity in the MVB pathway protein 402    3.48 0.136 1  
Increased at least fourfold W7X3N9 LITAF-like zinc ribbon domain protein 170 LITAF 35.21 Membrane 15.3 0.361 2  
Increased at least fourfold Q239Z2 Calpain family cysteine protease 1171 Calpain-15 31.67 Ubiquitous 16.3 0.196 8  
Only found or increased at least fourfold in FAP256A/B-KO cellsUniprot IDNameAmino acidsHuman homolog?% identityKnown localization?Percent coverage (highest of repeats)emPAI scoreNumber of peptides (average of FAP256A/B-KO)
Only in mutant Q66T67 Chlamydomonas - Heat shock protein 90C - False positive - one peptide from ABC transporter, one peptide from HSP82 180        
Only in mutant I7MLD8 14-3-3 family protein 14-3-3 beta/zeta 254 14-3-3 protein 46.94 Ubiquitous 46.1 1.43 7.3  
Only in mutant I7MIJ3 Phospholipid scramblase 270    5.19 0.099 1  
Only in mutant W7XFI6 Cation channel family transporter 2914 Voltage-dependent L-type calcium channel  43 Membrane 4.7 0.078 8.3  
Only in mutant I7M0U0 Mg2+-specific channel-like exchanger, putative 625 Sodium/potassium/calcium exchanger 3 30 Membrane 8.16 0.209 4.7  
Only in mutant Q9GPP3 Immobilization antigen LB 316    55.1 2.028 8  
Only in mutant Q951C1 Ymf66 446    6.28 0.057 1  
Only in mutant I7LX36 C2 NT-type domain-containing protein 832    1.2 0.03 1  
Only in mutant Q22AC6 PAS domain S-box protein 1177    0.77 0.021 1  
Only in mutant Q22NR4 Arrestin 331    11.2 0.105 1.3  
Only in mutant Q232M6 14-3-3 family epsilon domain protein 246 14-3-3 protein 47.03 Ubiquitous 40.7 0.714 5.3  
Only in mutant Q232G0 Immobilization antigen 316 EPH receptor B1 33.33 Membrane 55.1 1.488 5  
Only in mutant Q232G1 Immobilization antigen 148 EPH receptor B1 28.24 Membrane 50 2.302 2  
Only in mutant Q23JY5 Arrestin 391    5.63 0.114 1.7  
Only in mutant I7LT86 Transmembrane protein, putative 871    9.41 0.155 4.7  
Increased at least fourfold I7M849 ABC transporter family protein 1831 ABC3 31.97 Membrane 17 0.295 17  
Increased at least fourfold I7MJL8 Uncharacterized protein 851    3.06 0.05 1.7  
Increased at least fourfold Q234Y0 Uncharacterized protein 115    31.3 0.471 1.7  
Increased at least fourfold Q22KL2 Sel1 domain protein 766 Protein sel-1 homolog 2 28.74 Endoplasmic reticulum 4.31 0.056 1.7  
Increased at least fourfold I7MMI9 Multidrug resistance protein-like transporter family ABC domain protein 1289 ATP-dependent translocase ABCB1 36.38 Membrane 12.6 0.221 9.3  
Increased at least fourfold Q23Q30 Regulator of Vps4 activity in the MVB pathway protein 402    3.48 0.136 1  
Increased at least fourfold W7X3N9 LITAF-like zinc ribbon domain protein 170 LITAF 35.21 Membrane 15.3 0.361 2  
Increased at least fourfold Q239Z2 Calpain family cysteine protease 1171 Calpain-15 31.67 Ubiquitous 16.3 0.196 8  
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