Table 1.

List of proteins significantly absent and reduced at least fourfold from FAP256A/B-KO compared with CU428 along with their human homologs, known localization, percent coverage, emPAI score, and number of peptides

Absent or decreased at least fourfold in FAP256A/B-KO cellsUniprot IDNameAmino acidsHuman homolog?% identityKnown localization?Percent coverage (highest of repeats)emPAI scoreNumber of peptides (average of WT)
Absent I7MLF6 EF hand protein 1766 CCDC81 23.74 Centrosome 7.53 0.169 8.7 
Absent Q22US8 Uncharacterized protein 798    2.88 0.054 1.3 
Absent I7MKU9 Uncharacterized protein 1730    2.08 0.046 2.3 
Absent Q24IJ6 Tetratricopeptide repeat protein 519 TPR repeat protein 28 24.07 Microtubule organizing center 8.09 0.225 
Absent Q23FN3 FAP256 872 CEP104 29.05 Cilia and centriole 20.3 0.392 8.3 
Absent I7LVP2 Divergent AAA domain protein 273 SLFNL1 30.77 Sperm cells 16.5 0.438 3.3 
Absent I7MH51 Uncharacterized protein 474    10.1 0.123 1.7 
Absent I7LTB1 FAP256B 1017 CEP104 25.98 Cilia and centriole 2.75 0.015 0.7 
Decreased at least fourfold Q22Y55 Phosphatase 2A 579 PP2A 34.15 Ubiquitous 50.4 1.419 16.3 
Decreased at least fourfold Q229R5 Ser/Thr phosphatase 313 PP2A 70.1  17.3 0.497 3.7 
Decreased at least fourfold I7LX00 Group 1 truncated haemoglobin 121    56.2 2.929 
Decreased at least fourfold I7LVY1 Kinesin motor catalytic domain protein 1066 Kif16B 29.75 Early endosomes 14.9 0.284 7.7 
Decreased at least fourfold Q22D06 WD domain, G-beta repeat protein 2585 Dynein assembly factor with WDR repeat domains 34.2 Cilia 8.55 0.171 13.7 
Decreased at least fourfold Q24CF9 WD domain, G-beta repeat protein 2207 Dynein assembly factor with WDR repeat domains 25.82 Cilia 27.5 0.79 38 
Decreased at least fourfold I7M9E4 ATP:guanido phosphotransferase, carboxy-terminal domain protein 370 Creatine kinase 41.87 Ubiquitous 41.4 3.474 12.3 
Decreased at least fourfold Q23DC5 Thioredoxin glutathione reductase 491 Thioredoxin reductase 55.7 Ubiquitous 7.74 0.177 2.3 
Decreased at least fourfold I7LU65 C2 domain protein 841 Coiled-coil domain-containing protein 33 26.24 Peroxisome 9.99 0.278 5.7 
Decreased at least fourfold I7MMW4 STOP Protein 592    19.8 0.441 6.7 
Decreased at least fourfold Q22F01 Phospholipid scramblase 277    14.8 0.718 3.3 
Absent or decreased at least fourfold in FAP256A/B-KO cellsUniprot IDNameAmino acidsHuman homolog?% identityKnown localization?Percent coverage (highest of repeats)emPAI scoreNumber of peptides (average of WT)
Absent I7MLF6 EF hand protein 1766 CCDC81 23.74 Centrosome 7.53 0.169 8.7 
Absent Q22US8 Uncharacterized protein 798    2.88 0.054 1.3 
Absent I7MKU9 Uncharacterized protein 1730    2.08 0.046 2.3 
Absent Q24IJ6 Tetratricopeptide repeat protein 519 TPR repeat protein 28 24.07 Microtubule organizing center 8.09 0.225 
Absent Q23FN3 FAP256 872 CEP104 29.05 Cilia and centriole 20.3 0.392 8.3 
Absent I7LVP2 Divergent AAA domain protein 273 SLFNL1 30.77 Sperm cells 16.5 0.438 3.3 
Absent I7MH51 Uncharacterized protein 474    10.1 0.123 1.7 
Absent I7LTB1 FAP256B 1017 CEP104 25.98 Cilia and centriole 2.75 0.015 0.7 
Decreased at least fourfold Q22Y55 Phosphatase 2A 579 PP2A 34.15 Ubiquitous 50.4 1.419 16.3 
Decreased at least fourfold Q229R5 Ser/Thr phosphatase 313 PP2A 70.1  17.3 0.497 3.7 
Decreased at least fourfold I7LX00 Group 1 truncated haemoglobin 121    56.2 2.929 
Decreased at least fourfold I7LVY1 Kinesin motor catalytic domain protein 1066 Kif16B 29.75 Early endosomes 14.9 0.284 7.7 
Decreased at least fourfold Q22D06 WD domain, G-beta repeat protein 2585 Dynein assembly factor with WDR repeat domains 34.2 Cilia 8.55 0.171 13.7 
Decreased at least fourfold Q24CF9 WD domain, G-beta repeat protein 2207 Dynein assembly factor with WDR repeat domains 25.82 Cilia 27.5 0.79 38 
Decreased at least fourfold I7M9E4 ATP:guanido phosphotransferase, carboxy-terminal domain protein 370 Creatine kinase 41.87 Ubiquitous 41.4 3.474 12.3 
Decreased at least fourfold Q23DC5 Thioredoxin glutathione reductase 491 Thioredoxin reductase 55.7 Ubiquitous 7.74 0.177 2.3 
Decreased at least fourfold I7LU65 C2 domain protein 841 Coiled-coil domain-containing protein 33 26.24 Peroxisome 9.99 0.278 5.7 
Decreased at least fourfold I7MMW4 STOP Protein 592    19.8 0.441 6.7 
Decreased at least fourfold Q22F01 Phospholipid scramblase 277    14.8 0.718 3.3 

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