Table 3. Relative α-MyHC protein... | Rockefeller University Press

Table 3.

Relative α-MyHC protein expression in replated hPSC-CMs

	hESC-CMs									hiPSC-CMs
α-MyHC	35 d	37 d	35 + 2 d	39 d	35 + 4 d	42 d	35 + 7 d	49 d	35 + 14 d	20 d	18 + 2 d
Mean (%)	3.86	5.46	17.73	6.48	19.72	5.92	14.83	8.61	5.32	11.12	24.82
± SD (%)	2.27	0.98	6.38	2.02	6.52	1.15	1.26	6.37	1.93	7.51	6.26

	hESC-CMs	hiPSC-CMs
Mean (%)	3.86	5.46	17.73	6.48	19.72	5.92	14.83	8.61	5.32	11.12	24.82
± SD (%)	2.27	0.98	6.38	2.02	6.52	1.15	1.26	6.37	1.93	7.51	6.26

α-MyHC protein expression relative to total MyHC isoform protein expression (in percent) of replated and non-replated hESC- and hiPSC-CMs on indicated days. MyHC isoforms were separated by SDS-PAGE and MyHC isoform expression was determined densitometrically (see Fig. 4, D and F). Mean, hESC-CMs: n = 3 culture dishes from three differentiations; hiPSC-CMs: n = 3 or 4 culture dishes from one differentiation.

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