Table 1. Force-related steady-state and... | Rockefeller University Press

Table 1.

Force-related steady-state and kinetic parameters of myofibrils from replated hESC-CMs

Force-related parameters	35 d	+2 d	+3 d	+6 d	+7 d	+8 d	+9 d	+10 d
F_act (kPa)	42 ± 10	32 ± 11	26 ± 11	24 ± 2^{^a}	22 ± 9^{^a}	18 ± 6^{^a}	25 ± 10^{^a}	22 ± 10^{^a}
k_act (s⁻¹)	0.66 ± 0.14	1.56 ± 0.33^{^a}	1.64 ± 0.48	1.26 ± 0.27	0.75 ± 0.12	0.64 ± 0.02	0.69 ± 0.05	0.64 ± 0.02
k_tr (s⁻¹)	0.75 ± 0.10	2.01 ± 0.64^{^a}	1.84 ± 0.19	1.49 ± 0.44	0.72 ± 0.10	0.72 ± 0.03	0.88 ± 0.12	0.84 ± 0.15
k_lin (s⁻¹)	0.26 ± 0.06	0.64 ± 0.11^{^a}	0.57 ± 0.09^{^a}	0.52 ± 0.18	0.30 ± 0.08	0.25 ± 0.12	0.33 ± 0.13	0.30 ± 0.11
t_lin (ms)	197 ± 60	165 ± 61	134 ± 45	156 ± 51	191 ± 77	195 ± 92	199 ± 42	212 ± 46
k_rel (s⁻¹)	4.62 ± 0.60	4.69 ± 2.15	3.93 ± 0.04	4.80 ± 0.79	4.02 ± 1.16	4.10 ± 0.91	4.33 ± 2.28	4.43 ± 0.96

Force-related parameters	35 d	+2 d	+3 d	+6 d	+7 d	+8 d	+9 d	+10 d
F_act (kPa)	42 ± 10	32 ± 11	26 ± 11	24 ± 2^{^a}	22 ± 9^{^a}	18 ± 6^{^a}	25 ± 10^{^a}	22 ± 10^{^a}
k_act (s⁻¹)	0.66 ± 0.14	1.56 ± 0.33^{^a}	1.64 ± 0.48	1.26 ± 0.27	0.75 ± 0.12	0.64 ± 0.02	0.69 ± 0.05	0.64 ± 0.02
k_tr (s⁻¹)	0.75 ± 0.10	2.01 ± 0.64^{^a}	1.84 ± 0.19	1.49 ± 0.44	0.72 ± 0.10	0.72 ± 0.03	0.88 ± 0.12	0.84 ± 0.15
k_lin (s⁻¹)	0.26 ± 0.06	0.64 ± 0.11^{^a}	0.57 ± 0.09^{^a}	0.52 ± 0.18	0.30 ± 0.08	0.25 ± 0.12	0.33 ± 0.13	0.30 ± 0.11
t_lin (ms)	197 ± 60	165 ± 61	134 ± 45	156 ± 51	191 ± 77	195 ± 92	199 ± 42	212 ± 46
k_rel (s⁻¹)	4.62 ± 0.60	4.69 ± 2.15	3.93 ± 0.04	4.80 ± 0.79	4.02 ± 1.16	4.10 ± 0.91	4.33 ± 2.28	4.43 ± 0.96

Force-related steady-state (F_act) and kinetic parameters (k_act, k_tr, k_lin, t_lin, k_rel) of myofibrils from hESC-CMs replated for indicated days, and of non-replated (35 d) hESC-CMs (data imported from Iorga et al. [2018]). Data for most relevant cross-bridge-related parameters k_tr and k_lin are also shown in Fig. 2, C and D.

Significance level at least P < 0.05 or smaller.

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