Table 1. Fluorophore diffusion in BL6... | Rockefeller University Press

Table 1.

Fluorophore diffusion in BL6 murine myocytes

Fluorophore (MW, kD)	T (°C)	HR (nm)	F_myo/pip	F_bound	D_X (cm²/s)	D_X-free (cm²/s)	D_X-H2O (cm²/s)	Fold reduction
Bodipy-FL-ATP (0.88)	24	0.85	0.67	0.50	0.4 × 10⁻⁷	0.57 × 10⁻⁷	4.5 × 10⁻⁶^{^a}	>70
Carboxyfluorescein (0.38)	24	0.65	0.48	0.31	1.2 × 10⁻⁷	1.7 × 10⁻⁷	4.8 × 10⁻⁶^{^b}	28
Carboxyfluorescein (0.38)	34	0.65	0.52	0.36	3.2 × 10⁻⁷	5.0 × 10⁻⁷	4.8 × 10⁻⁶^{^b}	10
Sulforhodamine (0.56)	24	0.74	5.0	0.93	0.3 × 10⁻⁷	4.3 × 10⁻⁷	4.0–4.4 × 10⁻⁶^{^c}	10
TRITC-dextran (4.4)	24	1.44	0.74	0.55	0.4 × 10⁻⁷	0.78 × 10⁻⁷	1.5 × 10⁻⁶^{^d}	19
0.5 kD PEG (0.83)	24	0.84	0.38	0.12	1.1 × 10⁻⁷	1.2 × 10⁻⁷	3 × 10⁻⁶^{^e}	25
2 kD PEG (2.3)	24	1.16	0.42	0.21	0.5 × 10⁻⁷	1.3 × 10⁻⁷	6 × 10⁻⁷^{^e}	46
2 kD PEG-NH₃ (2.3)	24	1.16	0.48	0.31	0.33 × 10⁻⁷	0.48 × 10⁻⁷	---	----
10 kD PEG (10.3)	24	1.90	0.69	0.52	0.9 × 10⁻⁷	3.1 × 10⁻⁷	2.5 × 10⁻⁷^{^e}	8

Fluorophore (MW, kD)	T (°C)	HR (nm)	F_myo/pip	F_bound	D_X (cm²/s)	D_X-free (cm²/s)	D_X-H2O (cm²/s)	Fold reduction
Bodipy-FL-ATP (0.88)	24	0.85	0.67	0.50	0.4 × 10⁻⁷	0.57 × 10⁻⁷	4.5 × 10⁻⁶^a	>70
Carboxyfluorescein (0.38)	24	0.65	0.48	0.31	1.2 × 10⁻⁷	1.7 × 10⁻⁷	4.8 × 10⁻⁶^b	28
Carboxyfluorescein (0.38)	34	0.65	0.52	0.36	3.2 × 10⁻⁷	5.0 × 10⁻⁷	4.8 × 10⁻⁶^b	10
Sulforhodamine (0.56)	24	0.74	5.0	0.93	0.3 × 10⁻⁷	4.3 × 10⁻⁷	4.0–4.4 × 10⁻⁶^c	10
TRITC-dextran (4.4)	24	1.44	0.74	0.55	0.4 × 10⁻⁷	0.78 × 10⁻⁷	1.5 × 10⁻⁶^d	19
0.5 kD PEG (0.83)	24	0.84	0.38	0.12	1.1 × 10⁻⁷	1.2 × 10⁻⁷	3 × 10⁻⁶^e	25
2 kD PEG (2.3)	24	1.16	0.42	0.21	0.5 × 10⁻⁷	1.3 × 10⁻⁷	6 × 10⁻⁷^e	46
2 kD PEG-NH₃ (2.3)	24	1.16	0.48	0.31	0.33 × 10⁻⁷	0.48 × 10⁻⁷	---	----
10 kD PEG (10.3)	24	1.90	0.69	0.52	0.9 × 10⁻⁷	3.1 × 10⁻⁷	2.5 × 10⁻⁷^e	8

All values are a mean of four or more measurements. From left to right: Column 1: Fluorophore analyzed. Column 2: Temperature of observations. Column 3: Hydrodynamic radius. Column 4: Ratio of fluorescence of pipette solution to myoplasm in close proximity after >20 min (F_myo/pip). Values for BSA and GFP were 0.07 and 0.15, respectively, indicating exclusion from myoplasm. Column 5: Estimated fractions of fluorophores bound by cytoplasm, assuming the free cytoplasmic space to be one-third of total myocyte volume (Lu et al., 2016). $F_{b o u n d} = (F_{m y o / p i p} \times 3 - 1) / (F_{m y o / pip} \times 3)$ ⁠. Column 6: Estimated diffusion coefficients for total fluorophores. $λ_{m y o}^{2} / (2 τ)$ with λ_myo = 0.012 cm. Column 7: Diffusion coefficients for unbound fluorophore, estimated as $D_{x} / (1 - F_{b o u n d})$ ⁠. Column 8: Estimated diffusion coefficients for given compounds in free water. Column 9: Estimated fold reduction of diffusion coefficients of unbound compounds in murine myocyte cytoplasm versus water (D_x_-H2O/D_x_-free).

Fig.5.

Kramer et al., 2007.

Gendron et al., 2008 describes diffusion coefficients for multiple rhodamine derivatives.

Gribbon and Hardingham, 1998.

Waggoner et al., 1995.

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