Urea self-exchange permeability in human RBCs from 24 donors
| Donor . | Pu . | SD . | n . | Phloretin . | Pu,i . | SD . | n . | Inhibition . | Jk (a,b) . | Blood type AB0, Rh± . | Sex . | Donor age . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | cm/s × 104 . | . | mM . | cm/s × 106 . | . | % . | . | . | Years . | |||
| D57 | 6.14 | ±0.49 | 4 | ND | ND | ND | M | 55 | ||||
| D53 | 6.08 | ±0.47 | 4 | 1.00 | 1.71 | ±0.71 | 4 | 99.8 | −,+ | A,− | F | 75 |
| D52 | 5.65 | ±0.20 | 4 | 1.00 | 4.05 | ±0.88 | 3 | 99.3 | +,+ | 0,− | F | 77 |
| D59 | 5.44 | ±0.07 | 2 | ND | ND | ND | F | 36 | ||||
| D01 | 5.42 | ±0.86 | 4 | ND | ND | ND | NA | NA | ||||
| PBNa | 5.17 | ±0.02 | 3 | +,− | 0,+ | M | 28 | |||||
| D11 | 4.79 | ±0.42 | 4 | 1.25 | 0.76 | ±0.24 | 4 | 99.8 | −,+ | A,+ | NA | NA |
| D05 | 4.68 | ±0.22 | 4 | 0.50 | 3.39 | ±1.17 | 4 | 99.3 | −,+ | A,+ | F | 55 |
| D04 | 4.66 | ±0.57 | 4 | ND | +,− | 0,+ | M | 52 | ||||
| D03 | 4.59 | ±0.53 | 4 | ND | ND | A,+ | F | 52 | ||||
| D09 | 4.43 | ±0.66 | 4 | 1.00 | 1.45 | ±0.28 | 4 | 99.7 | +,− | 0,+ | M | 26 |
| D07 | 4.24 | ±0.31 | 4 | 1.00 | 1.29 | ±0.05 | 2 | 99.7 | ND | A,+ | M | 48 |
| D25 | 4.22 | ±0.45 | 4 | 1.00 | 3.39 | ±0.19 | 4 | 99.2 | +,− | 0,− | NA | NA |
| D08 | 4.15 | ±0.26 | 4 | 1.00 | 0.86 | ±0.30 | 4 | 99.8 | −,+ | A,+ | M | 57 |
| D06 | 3.94 | ±0.36 | 4 | 1.00 | 1.73 | ±0.35 | 4 | 99.6 | ND | B,+ | M | 26 |
| D10 | 3.87 | ±0.49 | 4 | 1.25 | 0.94 | ±0.18 | 4 | 99.8 | −,+ | 0,− | NA | NA |
| D02 | 3.78 | ±0.33 | 8 | 0.75 | 1.23 | ±0.72 | 13 | 99.7 | +,− | 0,+ | M | 40 |
| D12 | 3.23 | ±0.64 | 4 | 1.25 | 1.10 | ±0.15 | 4 | 99.7 | +,+ | A,+ | F | 51 |
| JBRa | 2.67 | ±0.07 | 4 | 99.4 | +,+ | A,+ | M | 38 | ||||
| D17 | 0.87 | ±0.03 | 4 | 1.25 | 3.96 | ±1.20 | 3 | 95.4 | +,+ | 0,+ | M | 67 |
| D20 | 0.62 | ±0.02 | 3 | 1.00 | 2.32 | - | 1 | 96.3 | ND | A,+ | F | 23 |
| JBR | 0.62 | ±0.15 | 4 | 1.00 | 1.02 | - | 1 | 98.4 | +,+ | A,+ | M | 75 |
| D51 | 0.59 | ±0.05 | 4 | 1.25 | 0.65 | ±0.32 | 2 | 98.9 | +,+ | B,+ | M | 67 |
| D13 | 0.58 | ±0.03 | 4 | 1.25 | 0.66 | ±0.24 | 4 | 98.9 | +,+ | A,+ | M | 31 |
| D21 | 0.57 | ±0.02 | 3 | 1.00 | 0.60 | ±0.09 | 3 | 98.9 | +,− | 0,+ | M | 40 |
| PBN | 0.53 | ±0.08 | 3 | 1.00 | 1.47 | ±0.60 | 2 | 97.2 | +,− | 0,+ | M | 65 |
| Donor . | Pu . | SD . | n . | Phloretin . | Pu,i . | SD . | n . | Inhibition . | Jk (a,b) . | Blood type AB0, Rh± . | Sex . | Donor age . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | cm/s × 104 . | . | mM . | cm/s × 106 . | . | % . | . | . | Years . | |||
| D57 | 6.14 | ±0.49 | 4 | ND | ND | ND | M | 55 | ||||
| D53 | 6.08 | ±0.47 | 4 | 1.00 | 1.71 | ±0.71 | 4 | 99.8 | −,+ | A,− | F | 75 |
| D52 | 5.65 | ±0.20 | 4 | 1.00 | 4.05 | ±0.88 | 3 | 99.3 | +,+ | 0,− | F | 77 |
| D59 | 5.44 | ±0.07 | 2 | ND | ND | ND | F | 36 | ||||
| D01 | 5.42 | ±0.86 | 4 | ND | ND | ND | NA | NA | ||||
| PBNa | 5.17 | ±0.02 | 3 | +,− | 0,+ | M | 28 | |||||
| D11 | 4.79 | ±0.42 | 4 | 1.25 | 0.76 | ±0.24 | 4 | 99.8 | −,+ | A,+ | NA | NA |
| D05 | 4.68 | ±0.22 | 4 | 0.50 | 3.39 | ±1.17 | 4 | 99.3 | −,+ | A,+ | F | 55 |
| D04 | 4.66 | ±0.57 | 4 | ND | +,− | 0,+ | M | 52 | ||||
| D03 | 4.59 | ±0.53 | 4 | ND | ND | A,+ | F | 52 | ||||
| D09 | 4.43 | ±0.66 | 4 | 1.00 | 1.45 | ±0.28 | 4 | 99.7 | +,− | 0,+ | M | 26 |
| D07 | 4.24 | ±0.31 | 4 | 1.00 | 1.29 | ±0.05 | 2 | 99.7 | ND | A,+ | M | 48 |
| D25 | 4.22 | ±0.45 | 4 | 1.00 | 3.39 | ±0.19 | 4 | 99.2 | +,− | 0,− | NA | NA |
| D08 | 4.15 | ±0.26 | 4 | 1.00 | 0.86 | ±0.30 | 4 | 99.8 | −,+ | A,+ | M | 57 |
| D06 | 3.94 | ±0.36 | 4 | 1.00 | 1.73 | ±0.35 | 4 | 99.6 | ND | B,+ | M | 26 |
| D10 | 3.87 | ±0.49 | 4 | 1.25 | 0.94 | ±0.18 | 4 | 99.8 | −,+ | 0,− | NA | NA |
| D02 | 3.78 | ±0.33 | 8 | 0.75 | 1.23 | ±0.72 | 13 | 99.7 | +,− | 0,+ | M | 40 |
| D12 | 3.23 | ±0.64 | 4 | 1.25 | 1.10 | ±0.15 | 4 | 99.7 | +,+ | A,+ | F | 51 |
| JBRa | 2.67 | ±0.07 | 4 | 99.4 | +,+ | A,+ | M | 38 | ||||
| D17 | 0.87 | ±0.03 | 4 | 1.25 | 3.96 | ±1.20 | 3 | 95.4 | +,+ | 0,+ | M | 67 |
| D20 | 0.62 | ±0.02 | 3 | 1.00 | 2.32 | - | 1 | 96.3 | ND | A,+ | F | 23 |
| JBR | 0.62 | ±0.15 | 4 | 1.00 | 1.02 | - | 1 | 98.4 | +,+ | A,+ | M | 75 |
| D51 | 0.59 | ±0.05 | 4 | 1.25 | 0.65 | ±0.32 | 2 | 98.9 | +,+ | B,+ | M | 67 |
| D13 | 0.58 | ±0.03 | 4 | 1.25 | 0.66 | ±0.24 | 4 | 98.9 | +,+ | A,+ | M | 31 |
| D21 | 0.57 | ±0.02 | 3 | 1.00 | 0.60 | ±0.09 | 3 | 98.9 | +,− | 0,+ | M | 40 |
| PBN | 0.53 | ±0.08 | 3 | 1.00 | 1.47 | ±0.60 | 2 | 97.2 | +,− | 0,+ | M | 65 |
The self-exchange permeability coefficients were determined at 1 mM, pH 7.2, and 25°C. The inhibition of the urea transporter was determined by means of acute exposure of the cells to phloretin. NA, not available; ND, not determined.
From Brahm (1983b).