Oligo sequences for FACS-seq analysis
| Oligos | Oligo sequence (5′–3′) |
|---|---|
| FACS-seq barcodea | AAGCAGTGGTATCAACGCAGAGTACXXXXXXXXXXTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN |
| FACS-seq TSO | TSOAAGCAGTGGTATCAACGCAGAGTGAATrGrGrG |
| FACS-seq TSO PCR | PCRAAGCAGTGGTATCAACGCAGAGT |
| FACS-seq lib PCR 1.0 | AATGATACGGCGACCACCGAGATCTACACGCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGT*A*CFACSseq |
| FACS-seq Custom Read1 | GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC |
| Oligos | Oligo sequence (5′–3′) |
|---|---|
| FACS-seq barcode | AAGCAGTGGTATCAACGCAGAGTACXXXXXXXXXXTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN |
| FACS-seq TSO | TSOAAGCAGTGGTATCAACGCAGAGTGAATrGrGrG |
| FACS-seq TSO PCR | PCRAAGCAGTGGTATCAACGCAGAGT |
| FACS-seq lib PCR 1.0 | AATGATACGGCGACCACCGAGATCTACACGCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGT*A*CFACSseq |
| FACS-seq Custom Read1 | GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC |
10 Xs in the FACS-seq barcode primer indicate a 10-nt barcode made up of unique sequences for each sample to be pooled.