Table 1.
Summary of steady-state mechanical measurements
MeasurementWT (4)WT (4)cTnIAla2 (4)cTnIAla2 (4)cMyBP-C null (4)cMyBP-C null (4)
Basal(+) PKABasal(+) PKABasal(+) PKA
PTot (mN mm−227.6 ± 7.7 35.4 ± 5.5 26.3 ± 5.6 34.3 ± 8.2 18.9 ± 3.7 35.7 ± 3.8 
Po (mN mm−224.1 ± 8.6 33.2 ± 4.8 23.5 ± 4.9 31.0 ± 8.1 16.2 ± 3.1 32.9 ± 3.3 
Prest (mN mm−23.4 ± 1.1 2.2 ± 0.6 2.7 ± 0.9 3.3 ± 1.3 2.7 ± 0.6 2.8 ± 0.5 
ktr (s−119.7 ± 1.4 23.2 ± 0.8 19.5 ± 0.9 27.7 ± 3.0 24.4 ± 1.7 31.0 ± 1.5 
Vmax (ML s−14.9 ± 0.4 5.4 ± 0.2 4.3 ± 0.4 5.2 ± 0.4 5.3 ± 0.1 5.3 ± 0.2 
 tWT (5) tWT (4) t3SA (6) t3SA (6) t3SD (6) t3SD (5) 
 Basal (+) PKA Basal (+) PKA Basal (+) PKA 
PTot (mN mm−227.3 ± 6.3 23.8 ± 3.8 18.6 ± 3.3 24.9 ± 1.7 20.8 ± 3.4 15.7 ± 3.2 
Po (mN mm−225.1 ± 5.7 22.1 ± 3.4 15.8 ± 3.5 22.3 ± 1.4 19.3 ± 3.3 14.8 ± 3.1 
Prest (mN mm−22.2 ± 0.5 1.6 ± 0.5 2.7 ± 0.8 2.5 ± 0.6 1.4 ± 0.4 1.0 ± 0.1 
ktr (s−121.0 ± 1.7 19.5 ± 0.2 23.2 ± 1.8 21.5 ± 1.3 20.8 ± 1.1 21.6 ± 2.9 
Vmax (ML s−15.4 ± 0.1 5.5 ± 0.1 5.3 ± 0.3 5.3 ± 0.3 5.6 ± 0.2 5.0 ± 0.3 
MeasurementWT (4)WT (4)cTnIAla2 (4)cTnIAla2 (4)cMyBP-C null (4)cMyBP-C null (4)
Basal(+) PKABasal(+) PKABasal(+) PKA
PTot (mN mm−227.6 ± 7.7 35.4 ± 5.5 26.3 ± 5.6 34.3 ± 8.2 18.9 ± 3.7 35.7 ± 3.8 
Po (mN mm−224.1 ± 8.6 33.2 ± 4.8 23.5 ± 4.9 31.0 ± 8.1 16.2 ± 3.1 32.9 ± 3.3 
Prest (mN mm−23.4 ± 1.1 2.2 ± 0.6 2.7 ± 0.9 3.3 ± 1.3 2.7 ± 0.6 2.8 ± 0.5 
ktr (s−119.7 ± 1.4 23.2 ± 0.8 19.5 ± 0.9 27.7 ± 3.0 24.4 ± 1.7 31.0 ± 1.5 
Vmax (ML s−14.9 ± 0.4 5.4 ± 0.2 4.3 ± 0.4 5.2 ± 0.4 5.3 ± 0.1 5.3 ± 0.2 
 tWT (5) tWT (4) t3SA (6) t3SA (6) t3SD (6) t3SD (5) 
 Basal (+) PKA Basal (+) PKA Basal (+) PKA 
PTot (mN mm−227.3 ± 6.3 23.8 ± 3.8 18.6 ± 3.3 24.9 ± 1.7 20.8 ± 3.4 15.7 ± 3.2 
Po (mN mm−225.1 ± 5.7 22.1 ± 3.4 15.8 ± 3.5 22.3 ± 1.4 19.3 ± 3.3 14.8 ± 3.1 
Prest (mN mm−22.2 ± 0.5 1.6 ± 0.5 2.7 ± 0.8 2.5 ± 0.6 1.4 ± 0.4 1.0 ± 0.1 
ktr (s−121.0 ± 1.7 19.5 ± 0.2 23.2 ± 1.8 21.5 ± 1.3 20.8 ± 1.1 21.6 ± 2.9 
Vmax (ML s−15.4 ± 0.1 5.5 ± 0.1 5.3 ± 0.3 5.3 ± 0.3 5.6 ± 0.2 5.0 ± 0.3 

All values are expressed as mean ± SEM, with the number of trabecular preparations listed in parentheses.

ktr, maximal rate of tension redevelopment at pCa 4.5; Po, maximal Ca2+-activated tension at pCa 4.5; Prest, Ca2+-independent tension at pCa 9.0; PTot, maximal Ca2+-activated tension (maximal Ca2+-activated tension plus Ca2+-independent tension); Vmax, maximal unloaded shortening velocity at pCa 4.5.

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