Table 5.
Primers for quantitative real-time RT-PCR
Gene nameSequence Fw (5′→3′)Sequence Rev (5′→3′)
Human 18S TGC​CCT​ATC​AAC​TTT​CGA​TG GAT​GTG​GTA​GCC​GTT​TCT​CA 
Human B-ACTIN GCC​GTC​TTC​CCC​TCC​ATC​GTG GGA​GCC​ACA​CGC​AGC​TCA​TTG​TAG​A 
Human PTCH1 CCA​CAG​AAG​CGC​TCC​TAC​AA TGT​TCC​AAT​TTC​CAC​TGC​CTG 
Human GLI1 AGA​GAG​ACC​AAC​AGC​TGC​AC GAG​GTG​AGA​TGG​ACA​GTG​CC 
Human GLI2 CCC​ACG​CTC​TCC​ATG​ATC​TC AGC​AGG​AAG​GCC​AAA​CAG​TC 
Mouse b-Actin GAG​CAC​AGC​TTC​TTT​GCA​GCT​CCT​T TGC​CAT​GTT​CAA​TGG​GGT​ACT​TCA​G 
Mouse Ptch1 TGC​TGT​GCC​TGT​GGT​CAT​CCT​GAT​T CAG​AGC​GAG​CAT​AGC​CCT​GTG​GTT​C 
Mouse Gli1 CAG​CAG​CTG​CAC​TGA​AGG​ATC​TC GCT​GGC​ATC​AGA​AAG​GGG​CG 
Gene nameSequence Fw (5′→3′)Sequence Rev (5′→3′)
Human 18S TGC​CCT​ATC​AAC​TTT​CGA​TG GAT​GTG​GTA​GCC​GTT​TCT​CA 
Human B-ACTIN GCC​GTC​TTC​CCC​TCC​ATC​GTG GGA​GCC​ACA​CGC​AGC​TCA​TTG​TAG​A 
Human PTCH1 CCA​CAG​AAG​CGC​TCC​TAC​AA TGT​TCC​AAT​TTC​CAC​TGC​CTG 
Human GLI1 AGA​GAG​ACC​AAC​AGC​TGC​AC GAG​GTG​AGA​TGG​ACA​GTG​CC 
Human GLI2 CCC​ACG​CTC​TCC​ATG​ATC​TC AGC​AGG​AAG​GCC​AAA​CAG​TC 
Mouse b-Actin GAG​CAC​AGC​TTC​TTT​GCA​GCT​CCT​T TGC​CAT​GTT​CAA​TGG​GGT​ACT​TCA​G 
Mouse Ptch1 TGC​TGT​GCC​TGT​GGT​CAT​CCT​GAT​T CAG​AGC​GAG​CAT​AGC​CCT​GTG​GTT​C 
Mouse Gli1 CAG​CAG​CTG​CAC​TGA​AGG​ATC​TC GCT​GGC​ATC​AGA​AAG​GGG​CG 

Fw, forward; Rev, reverse.

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