Table 4.
Primers for semiquantitative RT-PCR
Gene nameSequence Fw (5′→3′)Sequence Rev (5′→3′)Amplicon length
Human GAPDH CGA​CCA​CTT​TGT​CAA​GCT​CA AGG​GGT​CTA​CAT​GGC​AAC​TG 200 bp 
Human SHH TGA​TGA​ACC​AGT​GGC​CAG​G GTGGCCATCTTCGTCCCA 62 bp 
Human SMO CAG​TTT​CAG​CGG​TGC​CAA​C GGT​GAG​TGT​GTG​CAG​CAG​CT 74 bp 
Human PTCH1 TGTTCGGCATGATGGGC AGC​GAT​CAG​GAT​GAC​CAC​G 65 bp 
Human GLI1 GGC​ACC​ATC​CAT​TTC​TAC​AGT​G TGC​TTT​CCT​CCC​TGA​TGG​G 68 bp 
Human GLI2 AGT​TTG​TTC​TCG​GGT​GCT​CTG ACA​TCT​GTC​ATC​TGA​AGC​GGC 339 bp 
Human GLI3 CAC​CCT​CCT​CAT​CTT​TTC​CC GGT​GTG​GGG​AGA​TCC​TAA​TG 172 bp 
Human SUFU TTT​ACC​CTG​ACC​AGC​CGA​AC TGG​AAC​ACG​TAT​CGT​GCC​AA 319 bp 
Mouse Gapdh TCC​ACC​ACC​CTG​TTG​CTG​TA GAC​TTC​AAC​AGC​AAC​TCC​CAC 118 bp 
Mouse Shh CAG​CGG​CAG​ATA​TGA​AGG​GA GGT​GAT​GTC​CAC​TGC​TCG​AC 274 bp 
Mouse Smo GTG​TGA​GAA​TGA​CCG​AGT​GGA GAA​CAG​CGG​GTT​CTG​ACA​CT 268 bp 
Mouse Ptch1 TGT​GGC​TGA​GAG​CGA​AGT​TT AGT​GCT​GAG​TCC​AGG​TGT​TG 319 bp 
Mouse Gli1 TTC​CTA​CGG​CCA​TCT​CTC​CA AAT​CGA​ACT​CCT​GGC​TGC​AA 386 bp 
Mouse Gli2 AGA​CAC​CAG​GAG​GGA​AGG​TA CGA​GGC​TAA​AGA​GTC​CCC​TC 301 bp 
Mouse Gli3 GCC​CTC​GAC​GTC​TAG​TGA​TG GTT​GAT​GTA​GGG​GTG​TGG​GG 373 bp 
Mouse Sufu ACA​GGA​ACA​TGG​GGA​GTC​CT CAA​TGG​GCA​CTG​TCC​GTA​GT 465 bp 
Gene nameSequence Fw (5′→3′)Sequence Rev (5′→3′)Amplicon length
Human GAPDH CGA​CCA​CTT​TGT​CAA​GCT​CA AGG​GGT​CTA​CAT​GGC​AAC​TG 200 bp 
Human SHH TGA​TGA​ACC​AGT​GGC​CAG​G GTGGCCATCTTCGTCCCA 62 bp 
Human SMO CAG​TTT​CAG​CGG​TGC​CAA​C GGT​GAG​TGT​GTG​CAG​CAG​CT 74 bp 
Human PTCH1 TGTTCGGCATGATGGGC AGC​GAT​CAG​GAT​GAC​CAC​G 65 bp 
Human GLI1 GGC​ACC​ATC​CAT​TTC​TAC​AGT​G TGC​TTT​CCT​CCC​TGA​TGG​G 68 bp 
Human GLI2 AGT​TTG​TTC​TCG​GGT​GCT​CTG ACA​TCT​GTC​ATC​TGA​AGC​GGC 339 bp 
Human GLI3 CAC​CCT​CCT​CAT​CTT​TTC​CC GGT​GTG​GGG​AGA​TCC​TAA​TG 172 bp 
Human SUFU TTT​ACC​CTG​ACC​AGC​CGA​AC TGG​AAC​ACG​TAT​CGT​GCC​AA 319 bp 
Mouse Gapdh TCC​ACC​ACC​CTG​TTG​CTG​TA GAC​TTC​AAC​AGC​AAC​TCC​CAC 118 bp 
Mouse Shh CAG​CGG​CAG​ATA​TGA​AGG​GA GGT​GAT​GTC​CAC​TGC​TCG​AC 274 bp 
Mouse Smo GTG​TGA​GAA​TGA​CCG​AGT​GGA GAA​CAG​CGG​GTT​CTG​ACA​CT 268 bp 
Mouse Ptch1 TGT​GGC​TGA​GAG​CGA​AGT​TT AGT​GCT​GAG​TCC​AGG​TGT​TG 319 bp 
Mouse Gli1 TTC​CTA​CGG​CCA​TCT​CTC​CA AAT​CGA​ACT​CCT​GGC​TGC​AA 386 bp 
Mouse Gli2 AGA​CAC​CAG​GAG​GGA​AGG​TA CGA​GGC​TAA​AGA​GTC​CCC​TC 301 bp 
Mouse Gli3 GCC​CTC​GAC​GTC​TAG​TGA​TG GTT​GAT​GTA​GGG​GTG​TGG​GG 373 bp 
Mouse Sufu ACA​GGA​ACA​TGG​GGA​GTC​CT CAA​TGG​GCA​CTG​TCC​GTA​GT 465 bp 

Fw, forward; Rev, reverse.

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