Table 2.
Secondary antibodies
AntibodySourceDilutionManufacturerUse
Anti-mouse IgG, HRP Goat 1:5,000 Merck Millipore WB 
Anti-rabbit IgG, HRP Donkey 1:5,000 Merck Millipore WB 
Anti-goat IgG, HRP Donkey 1:3,000 Merck Millipore WB 
Anti-rat IgG, HRP Goat 1:5,000 Merck Millipore WB 
Anti-rabbit IgG, HRP Goat 1:5,000 Jackson ImmunoResearch WB 
Anti-mouse IgG, Alexa 488 Goat 1:1,000 Life Technologies ICC 
Anti-mouse IgG, Alexa 647 Goat 1:1,000 Life Technologies ICC 
Anti-rabbit IgG, Alexa 555 Goat 1:1,000 Life Technologies ICC 
Anti-rabbit IgG, Alexa 647 Goat 1:1,000 Life Technologies ICC, IHC 
Anti-rat IgG, Alexa 555 Goat 1:1,000 Life Technologies IHC 
Anti-chicken IgG, Alexa 555 Goat 1:1,000 Life Technologies IHC 
Anti-rabbit IgG, light chain-specific, HRP conjugate Mouse 1:5,000 Jackson ImmunoResearch WB (co-IP control) 
Protein G, HRP conjugate  1:5,000 Thermo Fisher Scientific WB (co-IP control) 
AntibodySourceDilutionManufacturerUse
Anti-mouse IgG, HRP Goat 1:5,000 Merck Millipore WB 
Anti-rabbit IgG, HRP Donkey 1:5,000 Merck Millipore WB 
Anti-goat IgG, HRP Donkey 1:3,000 Merck Millipore WB 
Anti-rat IgG, HRP Goat 1:5,000 Merck Millipore WB 
Anti-rabbit IgG, HRP Goat 1:5,000 Jackson ImmunoResearch WB 
Anti-mouse IgG, Alexa 488 Goat 1:1,000 Life Technologies ICC 
Anti-mouse IgG, Alexa 647 Goat 1:1,000 Life Technologies ICC 
Anti-rabbit IgG, Alexa 555 Goat 1:1,000 Life Technologies ICC 
Anti-rabbit IgG, Alexa 647 Goat 1:1,000 Life Technologies ICC, IHC 
Anti-rat IgG, Alexa 555 Goat 1:1,000 Life Technologies IHC 
Anti-chicken IgG, Alexa 555 Goat 1:1,000 Life Technologies IHC 
Anti-rabbit IgG, light chain-specific, HRP conjugate Mouse 1:5,000 Jackson ImmunoResearch WB (co-IP control) 
Protein G, HRP conjugate  1:5,000 Thermo Fisher Scientific WB (co-IP control) 

ICC, immunocytochemistry; IHC, immunohistochemistry; IP, immunoprecipitation; WB, Western blotting.

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