Table 2. Experimental results showing... | Rockefeller University Press

Table 2.

Experimental results showing the impact of altered mitochondrial Ca²⁺uptake on cytosolic Ca²⁺concentration.

Reference	Experimental model and protocol	Cytosolic transient amplitude	Cytosolic transient duration	Mitochondrial transient amplitude
Gillis, 1997	Mechanically skinned fibers Rhutenium Red	nd	delayed relaxation half time +176%	nd
Caputo and Bolanos., 2008	FDB fibers Electrical stimulation FCCP 2 μM	decreased −46%	no change	nd
Weiss et al., 2010	FDB fibers Electrical stimulation FCCP 2.5 μM	decreased −55%	prolonged −37% (rate constant)	nd
Yi et al., 2011	FDB fibers G93A SOD1 mutation Voltage clamp	increased +15.6%	no change	decreased −14.9%
Andrade et al., 2005	Intact EO muscles Electrical stimulation CCCP 10 μM	increased +72%	nd	nd
Ainbinder et al., 2015	FDB fibers Electrical stimulation Mfn2 downregulation	increased twitch +10% Tetanus + 30%	no change	decreased −30%
Eisner et al., 2014	FDB fibers Electrical stimulation Mfn1 KO and downreg	Increased decay with repeated stimulation	nd	decreased uptake
Mammucari et al., 2015	FDB fibers–caffeine MCU silencing	no change	nd	decreased −45%
Mammucari et al., 2015	FDB fibers–caffeine MCU upregulation	decreased −10%	nd	increased +76%
Gherardi et al. 2019	FDB fibers–caffeine MCU–constitutive KO	decreased −10%	nd	decreased to 0
Favaro et al., 2019	FDB fibers Electrical stimulation DRP1-KO (MCU upregulation)	decreased −9%	nd	increased +14.2%
Debattisti et al., 2019	FDB fibers Electrical stimulation MICU1 KO	trend to decrease	no change	decreased −30%
Butera et al., 2021	FDB fibers–caffeine PVA KO and MCU down regulation	increased +36%	nd	decreased −28%

Reference	Experimental model and protocol	Cytosolic transient amplitude	Cytosolic transient duration	Mitochondrial transient amplitude
Gillis, 1997	Mechanically skinned fibersRhutenium Red	nd	delayed relaxationhalf time +176%	nd
Caputo and Bolanos., 2008	FDB fibersElectrical stimulationFCCP 2 μM	decreased −46%	no change	nd
Weiss et al., 2010	FDB fibersElectrical stimulationFCCP 2.5 μM	decreased −55%	prolonged −37%(rate constant)	nd
Yi et al., 2011	FDB fibersG93A SOD1 mutationVoltage clamp	increased +15.6%	no change	decreased −14.9%
Andrade et al., 2005	Intact EO musclesElectrical stimulationCCCP 10 μM	increased +72%	nd	nd
Ainbinder et al., 2015	FDB fibersElectrical stimulationMfn2 downregulation	increasedtwitch +10%Tetanus + 30%	no change	decreased −30%
Eisner et al., 2014	FDB fibersElectrical stimulationMfn1 KO and downreg	Increased decay with repeated stimulation	nd	decreased uptake
Mammucari et al., 2015	FDB fibers–caffeineMCU silencing	no change	nd	decreased −45%
Mammucari et al., 2015	FDB fibers–caffeine MCU upregulation	decreased −10%	nd	increased +76%
Gherardi et al. 2019	FDB fibers–caffeineMCU–constitutive KO	decreased −10%	nd	decreased to 0
Favaro et al., 2019	FDB fibersElectrical stimulationDRP1-KO (MCU upregulation)	decreased −9%	nd	increased +14.2%
Debattisti et al., 2019	FDB fibersElectrical stimulationMICU1 KO	trend to decrease	no change	decreased −30%
Butera et al., 2021	FDB fibers–caffeinePVA KO and MCU downregulation	increased +36%	nd	decreased −28%

The percent variations are in some cases directly reported in the papers and in some cases recalculated from histograms or tables. nd, not determined.

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