Experimental results showing the impact of altered mitochondrial Ca2+uptake on cytosolic Ca2+concentration.
| Reference . | Experimental model and protocol . | Cytosolic transient amplitude . | Cytosolic transient duration . | Mitochondrial transient amplitude . | |
|---|---|---|---|---|---|
| Gillis, 1997 | Mechanically skinned fibers Rhutenium Red | nd | delayed relaxation half time +176% | nd | |
| Caputo and Bolanos., 2008 | FDB fibers Electrical stimulation FCCP 2 μM | decreased −46% | no change | nd | |
| Weiss et al., 2010 | FDB fibers Electrical stimulation FCCP 2.5 μM | decreased −55% | prolonged −37% (rate constant) | nd | |
| Yi et al., 2011 | FDB fibers G93A SOD1 mutation Voltage clamp | increased +15.6% | no change | decreased −14.9% | |
| Andrade et al., 2005 | Intact EO muscles Electrical stimulation CCCP 10 μM | increased +72% | nd | nd | |
| Ainbinder et al., 2015 | FDB fibers Electrical stimulation Mfn2 downregulation | increased twitch +10% Tetanus + 30% | no change | decreased −30% | |
| Eisner et al., 2014 | FDB fibers Electrical stimulation Mfn1 KO and downreg | Increased decay with repeated stimulation | nd | decreased uptake | |
| Mammucari et al., 2015 | FDB fibers–caffeine MCU silencing | no change | nd | decreased −45% | |
| FDB fibers–caffeine MCU upregulation | decreased −10% | nd | increased +76% | ||
| Gherardi et al. 2019 | FDB fibers–caffeine MCU–constitutive KO | decreased −10% | nd | decreased to 0 | |
| Favaro et al., 2019 | FDB fibers Electrical stimulation DRP1-KO (MCU upregulation) | decreased −9% | nd | increased +14.2% | |
| Debattisti et al., 2019 | FDB fibers Electrical stimulation MICU1 KO | trend to decrease | no change | decreased −30% | |
| Butera et al., 2021 | FDB fibers–caffeine PVA KO and MCU down regulation | increased +36% | nd | decreased −28% | |
| Reference . | Experimental model and protocol . | Cytosolic transient amplitude . | Cytosolic transient duration . | Mitochondrial transient amplitude . | |
|---|---|---|---|---|---|
| Gillis, 1997 | Mechanically skinned fibers Rhutenium Red | nd | delayed relaxation half time +176% | nd | |
| Caputo and Bolanos., 2008 | FDB fibers Electrical stimulation FCCP 2 μM | decreased −46% | no change | nd | |
| Weiss et al., 2010 | FDB fibers Electrical stimulation FCCP 2.5 μM | decreased −55% | prolonged −37% (rate constant) | nd | |
| Yi et al., 2011 | FDB fibers G93A SOD1 mutation Voltage clamp | increased +15.6% | no change | decreased −14.9% | |
| Andrade et al., 2005 | Intact EO muscles Electrical stimulation CCCP 10 μM | increased +72% | nd | nd | |
| Ainbinder et al., 2015 | FDB fibers Electrical stimulation Mfn2 downregulation | increased twitch +10% Tetanus + 30% | no change | decreased −30% | |
| Eisner et al., 2014 | FDB fibers Electrical stimulation Mfn1 KO and downreg | Increased decay with repeated stimulation | nd | decreased uptake | |
| Mammucari et al., 2015 | FDB fibers–caffeine MCU silencing | no change | nd | decreased −45% | |
| FDB fibers–caffeine MCU upregulation | decreased −10% | nd | increased +76% | ||
| Gherardi et al. 2019 | FDB fibers–caffeine MCU–constitutive KO | decreased −10% | nd | decreased to 0 | |
| Favaro et al., 2019 | FDB fibers Electrical stimulation DRP1-KO (MCU upregulation) | decreased −9% | nd | increased +14.2% | |
| Debattisti et al., 2019 | FDB fibers Electrical stimulation MICU1 KO | trend to decrease | no change | decreased −30% | |
| Butera et al., 2021 | FDB fibers–caffeine PVA KO and MCU down regulation | increased +36% | nd | decreased −28% | |
The percent variations are in some cases directly reported in the papers and in some cases recalculated from histograms or tables. nd, not determined.