Table 1. Kinetic parameters determined... | Rockefeller University Press

Table 1.

Kinetic parameters determined in isolated mitochondrial preparations from various cell types.

reference	Tissue	T°C	V_max nmol/mg/min	V_max mmol/L_mito/s	Affinity K_0.5 μM	nH	Threshold nM
Bragadin et al., 1979	Liver	20	900	5.76		2
Hutson et al., 1976	Liver	25	640	4.10	3.1
Vinogradov and Scarpa, 1973	Liver	24	480–780	3.07–4.99	55–70	1.63
Reed and Bygrave, 1975	Liver	0	19	0.12	2-4	1.7
McMillin-Wood et al., 1980	Heart	30	577–1750	3.69–11.21	74–189	1
Scarpa and Graziotti, 1973	Heart	26	480–840	3.07–5.38	30–90	2
Sembrovich et al., 1985	Skelet. muscle	37	Fast: 55	0.35	0.89	2
Sembrovich et al., 1985	Skelet. muscle	37	Slow: 158	1.01	2.51	3.5
Brinley et al., 1978*	Squid axon	17	200	1.28			200–300

reference	Tissue	T°C	V_max nmol/mg/min	V_max mmol/L_mito/s	Affinity K_0.5 μM	nH	Threshold nM
Bragadin et al., 1979	Liver	20	900	5.76		2
Hutson et al., 1976	Liver	25	640	4.10	3.1
Vinogradov and Scarpa, 1973	Liver	24	480–780	3.07–4.99	55–70	1.63
Reed and Bygrave, 1975	Liver	0	19	0.12	2-4	1.7
McMillin-Wood et al., 1980	Heart	30	577–1750	3.69–11.21	74–189	1
Scarpa and Graziotti, 1973	Heart	26	480–840	3.07–5.38	30–90	2
Sembrovich et al., 1985	Skelet. muscle	37	Fast: 55	0.35	0.89	2
Sembrovich et al., 1985	Skelet. muscle	37	Slow: 158	1.01	2.51	3.5
Brinley et al., 1978*	Squid axon	17	200	1.28			200–300

The experimental data on mitochondrial calcium uptake in nmol/mg protein/minute were converted to μmol/L_mito/s using a factor $Φ_{m i t o} = \frac{1}{60} \frac{\min}{s} \frac{10^{3}}{2.6} \frac{m g}{L_{m i t o}} = 6.41,$ or to μmol/L_fiber/s using a factor $Φ_{f i b e r} = \frac{1}{60} \frac{\min}{s} \frac{10^{3}}{2.6} \frac{m g}{L_{m i t o}} 0.05 \frac{L_{m i t o}}{L_{f i b e r}} = 0.32,$ since a ratio 1:20 links the two volume references, under the assumption that, in murine fast fibers, mitochondria volume is 5% of fiber volume (see Butera et al., 2021) and 1 mg protein corresponds to 2.6 μl of mitochondria (Schwerzmann et al., 1989). *, intact axon preparation.

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