Resting and active membrane properties of NT, Kv12.1-targeted, and Kv12.2-targeted shRNA-expressing SCN neurons during the day and at night
| . | Firing rate (Hz) . | Rin (GΩ) . | Vr (mV) . | ISI (ms) . | |
|---|---|---|---|---|---|
| Day ZT 7–12 | NT shRNA | 3.9 ± 0.5* n = 24 | 2.1 ± 0.1 n = 23 | −41.5 ± 0.7** n = 24 | 221 ± 4* n = 24 |
| Kv12.1-targeted shRNA | 3.3 ± 0.5 n = 13 | 1.8 ± 0.2 n = 11 | −41.5 ± 1.2 n = 13 | 213 ± 9 n = 13 | |
| Kv12.2-targeted shRNA | 3.8 ± 0.6 n = 18 | 2.3 ± 0.3 n = 18 | −40.5 ± 0.9 n = 18 | 243 ± 8 n = 18 | |
| Night ZT 18–24 | NT shRNA | 1.6 ± 0.3* n = 27 | 0.9 ± 0.1* n = 22 | −45.8 ± 1.0** n = 27 | 366 ± 24* n = 27 |
| Kv12.1-targeted shRNA | 4.7 ± 0.7 n = 13 | 1.6 ± 0.2 n = 9 | −38.7 ± 1.9 n = 13 | 187 ± 5 n = 13 | |
| Kv12.2-targeted shRNA | 3.6 ± 0.8 n = 13 | 1.9 ± 0.3 n = 12 | −42.6 ± 1.5 n = 13 | 234 ± 6 n = 13 | |
| . | Firing rate (Hz) . | Rin (GΩ) . | Vr (mV) . | ISI (ms) . | |
|---|---|---|---|---|---|
| Day ZT 7–12 | NT shRNA | 3.9 ± 0.5* n = 24 | 2.1 ± 0.1 n = 23 | −41.5 ± 0.7** n = 24 | 221 ± 4* n = 24 |
| Kv12.1-targeted shRNA | 3.3 ± 0.5 n = 13 | 1.8 ± 0.2 n = 11 | −41.5 ± 1.2 n = 13 | 213 ± 9 n = 13 | |
| Kv12.2-targeted shRNA | 3.8 ± 0.6 n = 18 | 2.3 ± 0.3 n = 18 | −40.5 ± 0.9 n = 18 | 243 ± 8 n = 18 | |
| Night ZT 18–24 | NT shRNA | 1.6 ± 0.3* n = 27 | 0.9 ± 0.1* n = 22 | −45.8 ± 1.0** n = 27 | 366 ± 24* n = 27 |
| Kv12.1-targeted shRNA | 4.7 ± 0.7 n = 13 | 1.6 ± 0.2 n = 9 | −38.7 ± 1.9 n = 13 | 187 ± 5 n = 13 | |
| Kv12.2-targeted shRNA | 3.6 ± 0.8 n = 13 | 1.9 ± 0.3 n = 12 | −42.6 ± 1.5 n = 13 | 234 ± 6 n = 13 | |
Values are means ± SEM; n = number of cells; Rin = input resistance; Vr = resting membrane potentials; ISI = interspike interval. Values in daytime and nighttime NT shRNA-expressing neurons are significantly (*P = 0.0001; **P < 0.004) different.