Primers used for knockout and ddPCR
| CRISPR MacroH2A1 gRNA . | 5′-CCTCAATAGCAAGCCATCCTGT-3′ . |
|---|---|
| HSV1_gB_qPCR_F1 | 5′-CCGTCAGCACCTTCATCGA-3′ |
| HSV1_gB_qPCR_R1 | 5′-CGCTGGACCTCCGTGTAGTC-3′ |
| HSV1_gB_probe1 | 6FAM-5′-CCACGAGATCAAGGACAGCGGCC-3′-BHQ1 |
| beta-globin_qPCR_F1 | 5′-TGAAGGCTCATGGCAAGAAA-3′ |
| beta-globin_qPCR_R1 | 5′-GCTCACTCAGTGTGGCAAAGG-3′ |
| beta-globin_probe2_hex | 5HEX/5′-TCCAGGTGAGCCAGGCCATCACTA-3′/3BHQ_1 |
| CRISPR MacroH2A1 gRNA . | 5′-CCTCAATAGCAAGCCATCCTGT-3′ . |
|---|---|
| HSV1_gB_qPCR_F1 | 5′-CCGTCAGCACCTTCATCGA-3′ |
| HSV1_gB_qPCR_R1 | 5′-CGCTGGACCTCCGTGTAGTC-3′ |
| HSV1_gB_probe1 | 6FAM-5′-CCACGAGATCAAGGACAGCGGCC-3′-BHQ1 |
| beta-globin_qPCR_F1 | 5′-TGAAGGCTCATGGCAAGAAA-3′ |
| beta-globin_qPCR_R1 | 5′-GCTCACTCAGTGTGGCAAAGG-3′ |
| beta-globin_probe2_hex | 5HEX/5′-TCCAGGTGAGCCAGGCCATCACTA-3′/3BHQ_1 |