Increase in side-chain volume compared to glycine
| Amino acid | Å3 |
| Hydrophobic amino acids | |
| Alanine (A) | 28.5 |
| Valine (V) | 79.9 |
| Leucine (L) | 106.6 |
| Phenylalanine (F) | 129.8 |
| Tyrosine (Y) | 133.5 |
| Tryptophan (W) | 167.7 |
| Hydrophilic uncharged amino acids | |
| Serine (S) | 28.9 |
| Threonine (T) | 56.0 |
| Asparagine (N) | 57.6 |
| Glutamine (Q) | 83.8 |
| wt amino acid at E321/E324 | |
| Glutamate (E) | 78.3 |
| Amino acid | Å3 |
| Hydrophobic amino acids | |
| Alanine (A) | 28.5 |
| Valine (V) | 79.9 |
| Leucine (L) | 106.6 |
| Phenylalanine (F) | 129.8 |
| Tyrosine (Y) | 133.5 |
| Tryptophan (W) | 167.7 |
| Hydrophilic uncharged amino acids | |
| Serine (S) | 28.9 |
| Threonine (T) | 56.0 |
| Asparagine (N) | 57.6 |
| Glutamine (Q) | 83.8 |
| wt amino acid at E321/E324 | |
| Glutamate (E) | 78.3 |
Values indicate the increase in side-chain volume compared to the side-chain volume of glycine, calculated from the volume of the indicated amino acid minus the volume of glycine. Amino acid volumes are from Creighton (1984).