Table I. Comparison of blood cell... | Rockefeller University Press

Table I.

Comparison of blood cell parameters in WT and Nfil3^−/− mice

Population	Marker and parameter	WT	Nfil3^−/−
PBMC	WBC, ×10³/µl	5.97 ± 2.55	7.48 ± 4.53
	RBC, ×10⁶/µl	9.06 ± 0.65	8.86 ± 1.83
	HGB, g/dl	14.83 ± 0.80	14.49 ± 1.15
	HCT, %	52.26 ± 2.97	50.36 ± 7.85
	MCV, fL	57.54 ± 2.07	56.68 ± 2.94
	MCH, pg	16.34 ± 0.28	16.50 ± 1.19
	MCHC, g/dl	28.41 ± 0.68	29.27 ± 3.73
	PLT, ×10³/µl	1311 ± 150	1279 ± 164
BM lymphocytes, ×10⁷		3.64 ± 1.09	3.49 ± 0.39
	B220⁺CD43⁺, %	14.28 ± 1.24	13.13 ± 0.76
	B220⁺CD25⁺, %	17.29 ± 2.76	19.0 ± 1.31
	B220⁺IgM⁺, %	16.55 ± 3.94	16.41 ± 3.41
Liver lymphocytes, ×10⁷		0.24 ± 0.05	0.23 ± 0.10
Lung lymphocytes, ×10⁷		0.29 ± 0.05	0.34 ± 0.10
Splenocytes, ×10⁷		6.60 ± 0.56	7.14 ± 1.245
	B220⁺CD3⁻, %	48.80 ± 1.22	46.51 ± 5.05
	B220⁺IgM⁺, %	34.64 ± 9.34	38.54 ± 4.69
	B220⁻CD3⁺, %	33.87 ± 1.86	34.40 ± 3.72
	CD8⁺CD4⁻, %	41.92 ± 1.75	42.33 ± 4.45
	CD8⁻CD4⁺, %	46.57 ± 2.22	45.86 ± 1.11
	TCR-αβ⁺, %	95.33 ± 0.61	95.83 ± 0.44
	TCR-γδ⁺, %	1.43 ± 0.56	1.37 ± 1.23
Thymocytes, ×10⁷		10.68 ± 2.23	10.24 ± 1.21
	CD4⁺CD8⁻, %	9.1 ± 0.20	9.54 ± 1.45
	CD4⁻CD8⁺, %	3.87 ± 0.61	2.94 ± 0.27
	CD4⁺CD8⁺, %	84.02 ± 1.29	84.48 ± 1.69
	CD4⁻CD8⁻, %	3.03 ± 0.58	3.01 ± 0.64
	CD25⁻CD44⁺, %	15.38 ± 4.37	19.05 ± 2.01
	CD25⁺CD44⁺, %	4.15 ± 1.86	5.17 ± 1.56
	CD25⁺CD44⁻, %	64.13 ± 4.63	62.42 ± 2.02
	CD25⁻CD44⁻, %	14.32 ± 2.73	13.31 ± 0.49

Population	Marker and parameter	WT	Nfil3^−/−
PBMC	WBC, ×10³/µl	5.97 ± 2.55	7.48 ± 4.53
	RBC, ×10⁶/µl	9.06 ± 0.65	8.86 ± 1.83
	HGB, g/dl	14.83 ± 0.80	14.49 ± 1.15
	HCT, %	52.26 ± 2.97	50.36 ± 7.85
	MCV, fL	57.54 ± 2.07	56.68 ± 2.94
	MCH, pg	16.34 ± 0.28	16.50 ± 1.19
	MCHC, g/dl	28.41 ± 0.68	29.27 ± 3.73
	PLT, ×10³/µl	1311 ± 150	1279 ± 164
BM lymphocytes, ×10⁷		3.64 ± 1.09	3.49 ± 0.39
	B220⁺CD43⁺, %	14.28 ± 1.24	13.13 ± 0.76
	B220⁺CD25⁺, %	17.29 ± 2.76	19.0 ± 1.31
	B220⁺IgM⁺, %	16.55 ± 3.94	16.41 ± 3.41
Liver lymphocytes, ×10⁷		0.24 ± 0.05	0.23 ± 0.10
Lung lymphocytes, ×10⁷		0.29 ± 0.05	0.34 ± 0.10
Splenocytes, ×10⁷		6.60 ± 0.56	7.14 ± 1.245
	B220⁺CD3⁻, %	48.80 ± 1.22	46.51 ± 5.05
	B220⁺IgM⁺, %	34.64 ± 9.34	38.54 ± 4.69
	B220⁻CD3⁺, %	33.87 ± 1.86	34.40 ± 3.72
	CD8⁺CD4⁻, %	41.92 ± 1.75	42.33 ± 4.45
	CD8⁻CD4⁺, %	46.57 ± 2.22	45.86 ± 1.11
	TCR-αβ⁺, %	95.33 ± 0.61	95.83 ± 0.44
	TCR-γδ⁺, %	1.43 ± 0.56	1.37 ± 1.23
Thymocytes, ×10⁷		10.68 ± 2.23	10.24 ± 1.21
	CD4⁺CD8⁻, %	9.1 ± 0.20	9.54 ± 1.45
	CD4⁻CD8⁺, %	3.87 ± 0.61	2.94 ± 0.27
	CD4⁺CD8⁺, %	84.02 ± 1.29	84.48 ± 1.69
	CD4⁻CD8⁻, %	3.03 ± 0.58	3.01 ± 0.64
	CD25⁻CD44⁺, %	15.38 ± 4.37	19.05 ± 2.01
	CD25⁺CD44⁺, %	4.15 ± 1.86	5.17 ± 1.56
	CD25⁺CD44⁻, %	64.13 ± 4.63	62.42 ± 2.02
	CD25⁻CD44⁻, %	14.32 ± 2.73	13.31 ± 0.49

Data shown are the mean ± SD of nine independent experiments using mice of 6–8 wk of age. No significant differences in the indicated cellular distributions were observed between WT and Nfil3^−/− mice (unpaired Student's t test).

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