Testing whether EC coupling properties are altered by exogenous Casq
| Conditions | R initial | R final | Peak flux | Amount | [Ca]SR initial | [Ca]SR final | n cells |
| mM s−1 | µM | µM | µM | ||||
| WT EGTA Casq | 1.33 (0.05) | 1.06 (0.04) | 61.2 (12.8) | 2,197 (320) | 547 (138) | 183 (25.8) | 10 |
| WT EGTA δAsp | 1.29 (0.02) | 1.04 (0.06) | 38.0 (8.44) | 1,246 (185) | 407 (23.0) | 170 (24.7) | 4 |
| WT BAPTA Casq | 1.29 (0.04) | 1.05 (0.05) | 123 (12.8) | 1,191 (76.5) | 448 (58.9) | 201 (26.8) | 14 |
| WT BAPTA δAsp | 1.22 (0.08) | 1.12 (0.07) | 113 (24.5) | 920 (101) | 416 (113) | 263 (59.7) | 8 |
| Null EGTA Casq | 1.16 (0.18) | 0.63 (0.08) | 46.3 (15.2) | 947 (88.6) | 404 (259) | 27.7 (18.7) | 3 |
| Null EGTA δAsp | 1.31 (0.06) | 0.71 (0.05) | 41.7 (7.39) | 1,334 (265) | 475 (117) | 45.3 (12.1) | 6 |
| Null BAPTA Casq | 1.38 (0.06) | 0.70 (0.08) | 86.3 (19.8) | 1,139 (70.6) | 658 (144) | 51.6 (24.6) | 6 |
| Null BAPTA δAsp | 1.33 (0.05) | 0.78 (0.03) | 137 (19.9) | 848 (114) | 564 (144) | 65.7 (9.27) | 8 |
| Conditions | R initial | R final | Peak flux | Amount | [Ca]SR initial | [Ca]SR final | n cells |
| mM s−1 | µM | µM | µM | ||||
| WT EGTA Casq | 1.33 (0.05) | 1.06 (0.04) | 61.2 (12.8) | 2,197 (320) | 547 (138) | 183 (25.8) | 10 |
| WT EGTA δAsp | 1.29 (0.02) | 1.04 (0.06) | 38.0 (8.44) | 1,246 (185) | 407 (23.0) | 170 (24.7) | 4 |
| WT BAPTA Casq | 1.29 (0.04) | 1.05 (0.05) | 123 (12.8) | 1,191 (76.5) | 448 (58.9) | 201 (26.8) | 14 |
| WT BAPTA δAsp | 1.22 (0.08) | 1.12 (0.07) | 113 (24.5) | 920 (101) | 416 (113) | 263 (59.7) | 8 |
| Null EGTA Casq | 1.16 (0.18) | 0.63 (0.08) | 46.3 (15.2) | 947 (88.6) | 404 (259) | 27.7 (18.7) | 3 |
| Null EGTA δAsp | 1.31 (0.06) | 0.71 (0.05) | 41.7 (7.39) | 1,334 (265) | 475 (117) | 45.3 (12.1) | 6 |
| Null BAPTA Casq | 1.38 (0.06) | 0.70 (0.08) | 86.3 (19.8) | 1,139 (70.6) | 658 (144) | 51.6 (24.6) | 6 |
| Null BAPTA δAsp | 1.33 (0.05) | 0.78 (0.03) | 137 (19.9) | 848 (114) | 564 (144) | 65.7 (9.27) | 8 |
Definitions of values in columns are as in Table I. Groupings of cells in Table I are further separated depending on the Casq variant fused to the biosensor. No significant differences were found in the properties of [Ca2+]SR and release flux between cells expressing D4cpv-Casq1 and D4cpv-δAsp. SEM values are shown in parentheses.