Constraining relative movements of M3–S2 and S2–M4 in GluN1 or GluN2A disrupts NMDA receptor gating
| Subunits | n | I | eq. Po | MCT | MOT |
| pA | ms | ms | |||
| N1/N2A | 7 | −9.4 ± 0.6 | 0.61 ± 0.05 | 5.1 ± 0.7 | 8.3 ± 0.8 |
| N1(C,C)/N2A | 8 | −9.0 ± 0.6 | 0.021 ± 0.005a | 94 ± 20a | 1.2 ± 0.1a |
| N1/N2A(C,C) | 9 | −8.1 ± 0.4 | 0.008 ± 0.001a | 230 ± 34ab | 1.4 ± 0.1a |
| N1/N2A DTT | 5 | −8.3 ± 0.2 | 0.74 ± 0.09 | 7.5 ± 3.7 | 23 ± 4.9c |
| N1(C,C)/N2A DTT | 5 | −7.7 ± 0.5 | 0.79 ± 0.08 | 2.4 ± 0.8 | 16 ± 5.3 |
| N1(C,C)/N2A DTT | 4 | −9.2 ± 0.8 | 0.96 ± 0.01 | 1.0 ± 0.1 | 30 ± 5.9 |
| Subunits | n | I | eq. Po | MCT | MOT |
| pA | ms | ms | |||
| N1/N2A | 7 | −9.4 ± 0.6 | 0.61 ± 0.05 | 5.1 ± 0.7 | 8.3 ± 0.8 |
| N1(C,C)/N2A | 8 | −9.0 ± 0.6 | 0.021 ± 0.005a | 94 ± 20a | 1.2 ± 0.1a |
| N1/N2A(C,C) | 9 | −8.1 ± 0.4 | 0.008 ± 0.001a | 230 ± 34ab | 1.4 ± 0.1a |
| N1/N2A DTT | 5 | −8.3 ± 0.2 | 0.74 ± 0.09 | 7.5 ± 3.7 | 23 ± 4.9c |
| N1(C,C)/N2A DTT | 5 | −7.7 ± 0.5 | 0.79 ± 0.08 | 2.4 ± 0.8 | 16 ± 5.3 |
| N1(C,C)/N2A DTT | 4 | −9.2 ± 0.8 | 0.96 ± 0.01 | 1.0 ± 0.1 | 30 ± 5.9 |
Mean values (±SEM) for single-channel current amplitudes (I), equilibrium open probability (eq. Po), MCT, and MOT. Idealization and MIL fitting with five closed and two to four open states was done in QuB. A sequential-state model for GluN1/GluN2A NMDA receptor gating (Kussius and Popescu, 2009) was used. eq. Po is defined as the fractional occupancy of the open states in the MIL-fitted single-channel recordings. For statistical analysis, the non-DTT and DTT-exposed receptors were considered separately, with the control being the respective values in GluN1/GluN2A.
P < 0.05, relative to GluN1/GluN2A.
P < 0.05, GluN1(C,C)/GluN2A relative to GluN1/GluN2A(C,C).
P < 0.05, GluN1/GluN2A relative to GluN1/GluN2A DTT.