The slope parameters from the analysis of wild-type C1 dwell time
| [Ca2+] | Slope parameter Δαk (mean ± SD) | No. of patches |
| M | mmHg−1 | |
| 10−5 | 0.011 ± 0.0038 | 3 |
| 10−6 | 0.017 ± 0.0086 | 5 |
| [Ca2+] | Slope parameter Δαk (mean ± SD) | No. of patches |
| M | mmHg−1 | |
| 10−5 | 0.011 ± 0.0038 | 3 |
| 10−6 | 0.017 ± 0.0086 | 5 |
The single-channel plots of normalized C1 dwell time versus pressure from the wild type at different [Ca2+] (10−6 and 10−5 M) were fitted with an exponential equation: normalized C1 dwell time = b × exp(−Δαk × P) (Fig. 2). The slope parameter Δαk here is remarkably similar to the slope parameter Δα from the ensemble current analysis in Table I, indicating that the transition from C1 to B is the major MS step.