Dwell time analysis of the wild-type single channel showing the effect of membrane stretch force on different states
| [Ca2+] | Normalized dwell times of different states under 150-mmHg pressure (mean ± SD) | No. of patches | ||
| C1 | C2 | O | ||
| M | ||||
| 10−5 | 0.21 ± 0.082 | 0.84 ± 0.24 | 1.3 ± 0.22 | 3 |
| 10−6 | 0.13 ± 0.031 | 1.0 ± 0.59 | 1.9 ± 0.78 | 5 |
| [Ca2+] | Normalized dwell times of different states under 150-mmHg pressure (mean ± SD) | No. of patches | ||
| C1 | C2 | O | ||
| 10−5 | 0.21 ± 0.082 | 0.84 ± 0.24 | 1.3 ± 0.22 | 3 |
| 10−6 | 0.13 ± 0.031 | 1.0 ± 0.59 | 1.9 ± 0.78 | 5 |
Dwell times in different states under 150-mmHg pressure at two different [Ca2+] normalized to those without pressure (Fig. 2 B, top). Unity would indicate no change. As shown clearly, C1 is significantly reduced by pressure, whereas C2 and O are mildly affected. Note that the missed short C2 likely caused an overestimation of O.
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