| Construct . | Single channel conductance (pS) . | n . |
|---|---|---|
| WT hENaC | 4.7 ± 1.0 | 6 |
| α(F62A)βγ | 4.5 ± 1.0 | 5 |
| α(F62A)β(Y29A)γ | 5.2 ± 0.3 | 5 |
| α(T66V)βγ | 7.1 ± 0.6a | 7 |
| α(T66V)β(T33V)γ | 8.1 ± 0.6b | 5 |
| Construct . | Single channel conductance (pS) . | n . |
|---|---|---|
| WT hENaC | 4.7 ± 1.0 | 6 |
| α(F62A)βγ | 4.5 ± 1.0 | 5 |
| α(F62A)β(Y29A)γ | 5.2 ± 0.3 | 5 |
| α(T66V)βγ | 7.1 ± 0.6a | 7 |
| α(T66V)β(T33V)γ | 8.1 ± 0.6b | 5 |
WT and mutant hENaC RNA was injected into Xenopus oocytes, and single-channel conductances were determined in the cell-attached patch-clamp configuration with 140 mM extracellular Na+. Single-channel conductances are presented as mean ± SD and were compared with WT ENaC using one-way ANOVA with Dunnett’s multiple comparisons test.
P < 0.05.
P < 0.05.